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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00000127
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: unc-119(ed3) III; niDf199 IV; armEx58.
Notes: armEx58 [WRM0611aH08-Del8mer + unc-119(+)]. Pick non-Unc to maintain. This strain contains a transgenic array that expresses a derivative WRM0611aH08 fosmid. The WRM0611aH08 fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. This derivative fosmid construct lacks the upstream 8-mer motif (CTGTTTCA) next to 21U-3372. The expression of this individual 21U-RNA is lost in transgenic animals. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45.|"Made_by: Cecere & Mansisidore"
Proper citation: RRID:WB-STRAIN:WBStrain00000127 Copy
http://www.wormbase.org/db/get?name=WBStrain00000129
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)|WBGene00006975(zfp-1)
Genomic Alteration: WBGene00006843(unc-119), WBGene00006975(zfp-1)
Availability: available
References:
Synonyms: zfp-1(ok554) unc-119(ed3) III; armEx14.
Notes: armEx14 [PHD1-PHD2::FLAG + zfp-1(short isoform) + unc-119(+)]. Pick non-Unc animals to maintain. The fosmid-based armEx14 transgene rescues zfp-1(ok554)/nDf17 lethality. Reference: Avgousti DC, et al. Mol Cell Biol. 2013 Mar;33(5):999-1015.|"Made_by: Cecere & Mansisidore"
Proper citation: RRID:WB-STRAIN:WBStrain00000129 Copy
http://www.wormbase.org/db/get?name=WBStrain00000167
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: unc-119(ed3) III; bafIs63.
Notes: bafIs63 [lin-42p(mut)::GFP + unc-119(+)]. lin-42p(mut)::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+); all potential DAF-12 binding sites have been mutated. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
Proper citation: RRID:WB-STRAIN:WBStrain00000167 Copy
http://www.wormbase.org/db/get?name=WBStrain00000168
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000908(daf-12)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000908(daf-12), WBGene00006843(unc-119)
Availability: available
References:
Synonyms: unc-119(ed3) III; daf-12(rh61rh411) X; bafIs62.
Notes: bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Array was crossed into strain ALF3 to create ALF82. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
Proper citation: RRID:WB-STRAIN:WBStrain00000168 Copy
http://www.wormbase.org/db/get?name=WBStrain00000165
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000908(daf-12)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000908(daf-12), WBGene00006843(unc-119)
Availability: available
References:
Synonyms: unc-119(ed3) III; daf-12(rh61rh411) X; bafIs9.
Notes: bafIs9 [daf-12::TAP (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. TAP tag inserted into daf-12 fosmid. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
Proper citation: RRID:WB-STRAIN:WBStrain00000165 Copy
http://www.wormbase.org/db/get?name=WBStrain00000212
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: ljSi2 II; unc-119(ed3) III.
Notes: ljSi2 [mec-7::GCaMP6m::SL2::TagRFP + unc-119(+)] II. GCaMP6m (13.693) and RFP expressed in touch receptor neurons (ALML/R, AVM, PVM, PLML/R). Dual expression of GCamp6m and RFP allows for ratio-metric corrections of motion artifacts. Reference: Cho Y, et al. Lab Chip. 2017 Jul 25;17(15):2609-2618.|"Made_by: Dr. William Schafer"
Proper citation: RRID:WB-STRAIN:WBStrain00000212 Copy
http://www.wormbase.org/db/get?name=WBStrain00000239
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: wamSi3 II; unc-119(ed3) III.
Notes: wamSi3 [eft-3p::mNeptune::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mNeptune from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 110.
Proper citation: RRID:WB-STRAIN:WBStrain00000239 Copy
http://www.wormbase.org/db/get?name=WBStrain00000245
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: wamSi118 II; unc-119(ed3) III.
Notes: wamSi118 [eft-3p::mCerulean3::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mCerulean3 from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 110.
Proper citation: RRID:WB-STRAIN:WBStrain00000245 Copy
http://www.wormbase.org/db/get?name=WBStrain00000241
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: wamSi7 II; unc-119(ed3) III.
Notes: wamSi7 [eft-3p::mTagRFP-T::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mTagRFP-T from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 110.
Proper citation: RRID:WB-STRAIN:WBStrain00000241 Copy
http://www.wormbase.org/db/get?name=WBStrain00000242
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: wamSi101 V; unc-119(ed3) III.
Notes: Supplementary_genotype wamSi101 (eft-3p::mTFP::unc-54 3'UTR + Cbr-unc-119 (+)) V unc-119(ed3) III|"wamSi101 [eft-3p::mTFP::unc-54 3'UTR + Cbr-unc-119(+)] V. Expresses a single copy of mTFP from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 110."
Proper citation: RRID:WB-STRAIN:WBStrain00000242 Copy
http://www.wormbase.org/db/get?name=WBStrain00000243
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: unc-119(ed3) III; wamSi103 V.
Notes: wamSi103 [eft-3p::mKO2::unc-54 3'UTR + Cbr-unc-119(+)] V. Expresses a single copy of mKO2 from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 110.
Proper citation: RRID:WB-STRAIN:WBStrain00000243 Copy
http://www.wormbase.org/db/get?name=WBStrain00000255
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003055(lon-1)|WBGene00003918(par-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00003055(lon-1), WBGene00003918(par-3), WBGene00006843(unc-119)
Availability: available
References:
Synonyms: lon-1(e185) par-3(e2074) unc-119(ed3) III; zuIs45 V; sDp3(III;f)
Notes: Made_by: Lawrence E. Small|"zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Pick wild-type to maintain. Worms carrying the sDp3 duplication are wild-type; animals that have lost the duplication are Lon Par (maternal effect lethal). Cross of JJ1473 and KK237. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231."
Proper citation: RRID:WB-STRAIN:WBStrain00000255 Copy
http://www.wormbase.org/db/get?name=WBStrain00000270
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: vizSi32 II; unc-119(ed3) III.
Notes: Made_by: Tokyo Furuta|"vizSi32 [cdk-2p(intron)::GFP::tbb-2 3 UTR + unc-119(+)] II. vizSi32 was inserted into ttTi5605 on Chr II using MosSci. Intron 1 of cdk-2 drives drives GFP expression in this transgene. Reference: Furuta T, et al. Development. 2018 May 14;145(10). pii: dev161042. doi: 10.1242/dev.161042."
Proper citation: RRID:WB-STRAIN:WBStrain00000270 Copy
http://www.wormbase.org/db/get?name=WBStrain00000271
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: vizSi34 II; unc-119(ed3) III.
Notes: Made_by: Tokyo Furuta|"vizSi34 [cdk-2p::GFP::tbb-2 3 UTR + unc-119(+)] II. Superficially wild-type. vizSi34 was inserted into ttTi5605 on Chr II using MosSci. Predicted cdk-2 promoter (from WormBase) drives GFP expression. Reference: Furuta T, et al. Development. 2018 May 14;145(10). pii: dev161042. doi: 10.1242/dev.161042."
Proper citation: RRID:WB-STRAIN:WBStrain00000271 Copy
http://www.wormbase.org/db/get?name=WBStrain00000268
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000898(daf-2)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000898(daf-2), WBGene00006843(unc-119)
Availability: available
References:
Synonyms: daf-2(e1370) unc-119(ed3) III; vizIs23.
Notes: Made_by: Jessica (Jie) Chen|"vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40."
Proper citation: RRID:WB-STRAIN:WBStrain00000268 Copy
http://www.wormbase.org/db/get?name=WBStrain00000284
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
References:
Synonyms: unc-119(ed3) meT8 (III); meIs4 meT8 (IV); meIs1.
Notes: meIs1 [pie-1p::GFP::lacI + unc-119(+)]. meIs4 [lac-O + rol-6(su1006) + lacO] IV. Pick Rol worms to maintain. This strain throws both Rol and non-Rol worms, seemingly due to random silencing of rol-6(su1006) in the lacO array, meIs4. The strain expresses GFP::LacI in the gonad and embryos that is observed as foci (of lacO target) and nuclear haze. The expression level of GFP::LacI occasionally becomes low possibly due to random silencing of meIs1. If this happens, heat shock the strain at 25C for 3 days, and pick a clone that exhibits bright GFP signals. Even at the highest expression level, GFP signal is too weak to detect with a fluorescent dissection microscope, and it is necessary to use a regular compound fluorescent microscope with an oil immersion 60X or 100X objective. The NA of the objective should be higher than 1.4. Reference: Bilgir C, et al. G3 (Bethesda). 2013 Mar 11. pii: g3.112.005165v1.
Proper citation: RRID:WB-STRAIN:WBStrain00000284 Copy
http://www.wormbase.org/db/get?name=WBStrain00000336
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: EMPTY
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00000336 Copy
http://www.wormbase.org/db/get?name=WBStrain00000337
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: EMPTY
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00000337 Copy
http://www.wormbase.org/db/get?name=WBStrain00000338
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: EMPTY
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00000338 Copy
http://www.wormbase.org/db/get?name=WBStrain00000332
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: EMPTY
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00000332 Copy
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