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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[∆::neo2]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: Neo2 cassettes KO HHT2
Availability:
References:
Synonyms:
Notes: HHT2 knockout, neo 2 cassette.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02792 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ S1V,K5R,K8R,K10R,k12R, K17R]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
Availability:
References:
Synonyms:
Notes: G4B1G10A x G4B3C1A. HTA.1 S1P plus all K changed to R in N-terminus used to rescue ∆HTA1∆HTA2 double knockout heterokaryon. Abolishes all acetylation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02393 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA1 (now HTAX) is WT but HTA2 (now HTA1) has neo2; pm-s?, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
Availability:
References:
Synonyms:
Notes: G4B1G10A x G4B3C1A. wt HTA1 (pXL53) rescue ∆HTA1∆HTA2 double knockout heterokaryon. Assort to pm-s? Mates with HTA1-D1M01
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02394 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02314 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00932 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo2]/ hhf1[∆::neo2] (mpr1-1; mp-r, ?)
Affected Genes: HHF1 (TTHERM_00498190)
Genomic Alteration: Micronucleus: HHF1 ko with neo2 cassette
Availability:
References:
Synonyms:
Notes: ∆H4I gl KO homozygous heterokaryon
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02322 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2] (HTAX/htax[∆neo2];hta1[∆::neo2]; HTB1/htb1[∆::neo2]; htb2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 of HTAX, HTA1, HTB1, HTB2 Macronucleus: WT HTAX and HTB1, neo2 of HTA1, HTB2
Availability:
References:
Synonyms:
Notes: HTX1/HTB1 double wt rescue; Rescue of h∆∆HTA∆∆HTB-FBD1F6 and FBB1D3 with Dryl's.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02166 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT3; cy-r, I)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01992 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-3/mat1-3; serH-4/serH-4 (mat 1-3; serH-4, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01752 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cnjB[∆?::neo3]/ cnjB[∆?::neo3] (cnjB/cnjB
; wt)
Affected Genes: cnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: CnjB part of coding replaced with neo3
Availability:
References:
Synonyms:
Notes: Homozygous heterkaryon for ∆CnjB. Mates with ∆CnjB 2-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02049 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding, not sure if complete
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01911 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (pm-s, mp-r, wt HHT, ?)
Affected Genes: HHT1, HHT2, HHF1 (TTHERM_00570560, TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo3 KO of HHT1, HHT2/HHF2
Availability:
References:
Synonyms:
Notes: Germline homozygous heteokaryon for major H3 genes
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01881 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/btu1-1[K350M] (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1,T146G),HAc]; tax-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
Availability:
References:
Synonyms:
Notes: pT146G HA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 (T146G) with HA tag under control of MTT1 promoter. #1 and #5 are two separate single cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01366 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr10-1[∆::NEO2] (WT; WT, ?)
Affected Genes: SFR10 (TTHERM_00392670)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Availability:
References:
Synonyms:
Notes: Mates with UCB401
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03300 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (Circular permutation of HHO1 charge patch (see paper); pm-r, ?)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Somatic transformation of CU428 with HHO1 construct A-C
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02219 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-29; Basal body disorganization (TS)., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-29
Availability:
References:
Synonyms:
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 K29A, E30A, D33A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03274 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Nulli 2 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00840 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb2[∆::neo2]/htb2[∆::neo2] (mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: Neo2 into HTB2 replacing coding Macronucleus: HTB2 coding replaced by neo2 but assorted to pm-s
Availability:
References:
Synonyms:
Notes: This is the HTB2 germline KO homozygous heterokaryon. The 1.* series have different mating type from 10.* series.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02104 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,GFPc]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: C-terminal GFP- tag of HHT3, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: Somatic replace HHT3 gene with HHT3-GFP sequence. Neo2 in 5’ flanking of HHT3.
Mac transformant,GFP tagged HHT3, green nuclei.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01779 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi111[∆?::neo3] (twi11[∆?::neo3]/twi111[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Availability:
References:
Synonyms:
Notes: ∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01811 Copy
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