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http://www.wormbase.org/db/get?name=WBStrain00054696

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020706(atg-9)
Genomic Alteration: WBGene00020706(atg-9)
Availability: unknown
References:
Synonyms: atg-9(ola511[delta AP]) V.
Notes: Made_by: InVivo Biosystems|"ola511 is aCRISPR-engineered allele deleting a conserved sorting motif in ATG-9, causing a 2- to 3-fold decrease in LGG-1-containing puncta (and therefore autophagosomes) in the AIY neurites. Reference: Yang S, et al. Neuron. 2022 Mar 2;110(5):824-840.e10."

Proper citation: RRID:WB-STRAIN:WBStrain00054696 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054692

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003285(mir-57)|WBGene00003514(myo-2)|WBGene00005016(sqt-1)
Genomic Alteration: WBGene00003285(mir-57), WBGene00003514(myo-2), WBGene00005016(sqt-1)
Availability: unknown
References:
Synonyms: mir-57(umn34[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) II.
Notes: Made_by: Julie Knott & Marcus Vargas|"mir-57 pre-miRNA deletion strain deletion allele in which mir-57 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.]"

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•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054735

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00010785(top-2)
Genomic Alteration: WBGene00010785(top-2)
Availability: unknown
References:
Synonyms: ieSi57 ers55[top-2::degron::GFP] II.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Degron tag inserted into the endogenous top-2 locus. ieSi57 is a single-copy transgene insertion into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of target proteins in somatic tissues. Reference: Morao AK, et al. Mol Cell. 2022 Nov 17;82(22):4202-4217.e5. doi: 10.1016/j.molcel.2022.10.002. PMID: 36302374.|"Made_by: Ana Morao"

Proper citation: RRID:WB-STRAIN:WBStrain00054735 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054699

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000962(dhc-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000962(dhc-1), WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: dhc-1(lt45[dhc-1::GFP]) I; ltSi953 II; unc-119(ed3) III.
Notes: ltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous dhc-1 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged dhc-1 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826.

Proper citation: RRID:WB-STRAIN:WBStrain00054699 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054732

Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: rhIs2.
Notes: rhIs2 [pat-3::HA::GFP]. rhIs2 contains cosmid-derived full-length pat-3, including 5 kb 5UTR and 1 kb 3 UTR, with HA and GFP(S65C) tags inserted prior to the pat-3 stop codon. Reference: Plenefisch JD, et al. Development. 2000 127(6):1197-207. doi: 10.1242/dev.127.6.1197.

Proper citation: RRID:WB-STRAIN:WBStrain00054732 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054731

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018976(daam-1)
Genomic Alteration: WBGene00018976(daam-1)
Availability: unknown
References:
Synonyms: daam-1(ups39) V.
Notes: Superficially wild-type. ups39 is a CRISPR-engineered deletion within daam-1. daam-1(ups39) encodes an in-frame stop codon near the start of its FH2-coding sequence, and a 1-nt frame shift due to the LoxP site, and is thus predicted encode a non-functional formin. Reference: Sundaramurthy S, et al. Cytoskeleton (Hoboken). 2020 Oct;77(10):422-441. doi: 10.1002/cm.21639. PMID: 33103378.

Proper citation: RRID:WB-STRAIN:WBStrain00054731 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054734

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001086(dpy-27)
Genomic Alteration: WBGene00001086(dpy-27)
Availability: unknown
References:
Synonyms: ieSi57 II; ers54[dpy-27::degron::GFP] III.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Degron::GFP tag inserted into the endogenous dpy-27 locus. Dumpy, Him, X chromosome dosage compensation hypomorph. ieSi57 is a single-copy transgene insertion into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of target proteins in somatic tissues. Reference: Morao AK, et al. Mol Cell. 2022 Nov 17;82(22):4202-4217.e5. doi: 10.1016/j.molcel.2022.10.002. PMID: 36302374.|"Made_by: Ana Morao"

Proper citation: RRID:WB-STRAIN:WBStrain00054734 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054684

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001423(fib-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001423(fib-1), WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: muIs252 II; unc-119(ed3) III; fib-1(mu498[wrmScarlet11::fib-1]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054684 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054686

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001423(fib-1)
Genomic Alteration: WBGene00001423(fib-1)
Availability: unknown
References:
Synonyms: muIs257 I; fib-1(mu498[wrmScarlet11::fib-1]) V.
Notes: muIs257 [myo-3p::wrmScarlet1-10::unc-54 3'UTR] I. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4610. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054686 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054685

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001877(his-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001877(his-3), WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: muIs252 II; unc-119(ed3) III; his-3(mu500[his-3::wrmScarlet11(x3)]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged his-3::wrmScarlet11(x3) generated via CRISPR/Cas9 insertion of three wrmScarlet11 tags into the endogenous his-3 locus in parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054685 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054680

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003230(mex-5)|WBGene00004896(smu-2)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00003230(mex-5), WBGene00004896(smu-2), WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III.
Notes: Made_by: Dmytro Kukhtar|"Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4."

Proper citation: RRID:WB-STRAIN:WBStrain00054680 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054681

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)|WBGene00013025(vha-13)
Genomic Alteration: WBGene00006843(unc-119), WBGene00013025(vha-13)
Availability: unknown
References:
Synonyms: muIs252 II; unc-119(ed3) III; vha-13(mu493[wrmScarlet11::vha-13]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::vha-13 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054681 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054724

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001207(egl-43)
Genomic Alteration: WBGene00001207(egl-43)
Availability: unknown
References:
Synonyms: bmdSi363 I; egl-43(bmd88[egl-43p::egl-43::LoxP::GFP::egl-43]) II.
Notes: bmdSi363 [^SEC^ser-2p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Pick Rollers to maintain. Wild-type growth. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles.|"Made_by: Yutong Xiao"

Proper citation: RRID:WB-STRAIN:WBStrain00054724 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054727

Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: bmdSi245 swsn-8(bmd222[(swsn-8p::swsn-8::GFP]) I.
Notes: bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Wild-type growth and movement. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles.|"Made_by: Yutong Xiao"

Proper citation: RRID:WB-STRAIN:WBStrain00054727 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054723

Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: bmdSi348 I; bmdSi362 II.
Notes: bmdSi348 [loxN::rgef-1p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. bmdSi362 [loxN::rpl-28p::FRT3-LCK::mNG-STOP::FRT3::TIR1(F79G)::2A::PH::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. FLP-ON::TIR1 system for AID-tagged protein degradation in neurons. High levels of TIR1(F79G) expression in neurons by rgef-1p::FLP with co-expression of membrane markers. bmdSi362 contains the ubiquitous rpl-28 promoter driving expression of FRT3-LCK::mNG-STOP::FRT3::TIR1(F79G)::2A::PH::2xmKate2 construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https:|"Made_by: Matus Lab"

Proper citation: RRID:WB-STRAIN:WBStrain00054723 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054729

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004055(pmk-1)
Genomic Alteration: WBGene00004055(pmk-1)
Availability: unknown
References:
Synonyms: pmk-1(re170[pmk-1::mNG::3xFlag]) IV.
Notes: Made_by: Hanna Shin|"mNeonGreen and 3xFlag tag inserted at 3' end of endogenous pmk-1 locus. Fluorescent green signal detected in both cytosol and nuclei of all somatic cells; might be silenced in the germ line. Generated in an N2 background. Reference: Shin H, et al. Cell Rep. 2018 Sep 4;24(10):2669-2681. PMID: 30184501"

Proper citation: RRID:WB-STRAIN:WBStrain00054729 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054673

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006724(ubh-4)
Genomic Alteration: WBGene00006724(ubh-4)
Availability: unknown
References:
Synonyms: ubh-4(cer25[F73V]) II.
Notes: Made_by: Carmen Martnez-Fernndez|"Superficially wild-type. ubh-4(cer25[F73V]) is a missense mutation mimicking a human BAP1 cancer mutation. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270"

Proper citation: RRID:WB-STRAIN:WBStrain00054673 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054674

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006724(ubh-4)
Genomic Alteration: WBGene00006724(ubh-4)
Availability: unknown
References:
Synonyms: ubh-4(cer27) II.
Notes: Made_by: Carmen Martnez-Fernndez|"Reduced brood size. Genetic interaction with rpn-9. cer27 is a 1033 bp deletion removing the start codon and nearly all of the ubh-4 coding sequence. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270"

Proper citation: RRID:WB-STRAIN:WBStrain00054674 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054670

Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019779(endu-2)
Genomic Alteration: WBGene00019779(endu-2)
Availability: unknown
References:
Synonyms: endu-2(tm4977) X; byEx1551.
Notes: byEx1551 [vha-6p::endu-2::eGFP::3xFLAG + myo-2p::mCherry]. Pick mCherry+ animals to maintain array. Transgene provides intestinal rescue of endu-2(tm4977) that also rescues mortal germline (Mrt) phenotype. Reference: Qi W, et al. (2020) A secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans. BioRxiv. doi: 10.1101/2020.12.04.408260. Accepted by Nature Communications.

Proper citation: RRID:WB-STRAIN:WBStrain00054670 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00054714

Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: bmdSi297 II.
Notes: bmdSi297 [loxN::rpl-28p::FRT3::STOP::FRT3::TIR1(F79G)::T2A::DHB::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. Ubiquitous rpl-28 promoter driving expression of FRT3::STOP::FRT3::TIR1(F79G)::DHB construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https:|"Made_by: Matus Lab"

Proper citation: RRID:WB-STRAIN:WBStrain00054714 Copy   

•   Source: Integrated Animals