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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1 (GAL1/GAL1; gal-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: This is the strain used for mac genome sequencing project.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01539 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1,GFPc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal GFP tag into MTT1 locus, partial replacement
Availability:
References:
Synonyms:
Notes: In mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region with GFP tag . Use the pTTMGG construct (GTU coding, GFP tag driven by MTT promoter) to rescue GTU KO heterokaryon.
Requires Cd to grow, Use .25 to 1 ug/ml. GFP not detected at protein level.
Proper citation: RRID:TSC_SD01417 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4/R,K7R,K10R,K13R,K21R]; pm-r, ?)
Affected Genes: HTA3 (TTHERM_00143660 )
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4R, K7R, K10R, K13R, K21R.
Availability:
References:
Synonyms:
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTA3 K4R,K7R,K10R,K13R, K21R. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02070 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1::neo4/drh1::neo4. Neo in 5’end of gene,some coding remains (drh1[∆?::neo4 ] Neo in 5’end of gene,some coding remains; pm-r with Cd, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 Macronucleus: neo4
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ H7-4E.
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01944 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, R285D,K286D), HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation R285D, K286D and HA tag at c-teminus into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: rescued GTUKO 5x6 progeny having mutation R285D,K286D in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce. Not single celled yet. They were ~2 weeks old.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02516 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2] (mp-r, VII)
Affected Genes: HTB1 (TTHERM_00633360)
Genomic Alteration: Micronucleus: Neo2 into HTB1 replacing coding
Availability:
References:
Synonyms:
Notes: This is the HTB1 germline KO homozygous heterokaryon. The 9.* series have different mating type from 11.* series.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02080 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A
Availability:
References:
Synonyms:
Notes: IFT122 KO heterokaryon, this is the non- star side
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01791 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 3 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01031 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E433D,E435D]; pm-r)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU1 has only (E431, 432, 433, 435D) no WT
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,432,433,435D)
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02122 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HA-His-n]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA-His- tag of HHT3, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: HA-His-tagged hv2 transformant. Checked by western blot. HA-His-hv2 is expressed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02643 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K4Q] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-K4Q.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02091 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 3L (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deletion on left arm of chromosome 3.
Proper citation: RRID:TSC_SD01040 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 7/25/2009 from the pond designated Childs Bog in Harrisville, NH (lat. 42 57.763, long. -72 07.451, elevation 1388 ft, nearest road is Silver Lake Rd / Nelson).
Geographical area: New England
Collection number: 1187
Has a micronucleus.
Proper citation: RRID:TSC_SD01564 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX!/CHX1; mpr1?/mpr1? (mp-r, VII)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette. It can mate with HTA1 G1 15B.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02811 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BLT11-4288,BTU1429-443)], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Availability:
References:
Synonyms:
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pBbc-8 construct. BLT1(AA 1-428) with BTU1 at C-terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02260 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-1/janA-1; eja1-1/eja1-1; cdaK-1/cdaK-1 (janA-1; eja1-1; cdaK-1; janA, eja1,cdaK1, VII)
Affected Genes: JANA (janusA), EJA1 (enhancer of janA), CDAK (cell division arrest K)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Close to additive expression. However, the abnormalities of cytokinesis caused by expression of cdaK-1 are not as pronounced around the secondary oral meridian as they are around the primary orial meridian of janus-expressing cells. A clone with high expression of janA was selected, making the inclusion of eja1-1 likely.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01572 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-3/hpo1-3 (hpo1-3, II)
Affected Genes: HPO1 (hypoangular)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. The phenotype becomes more extreme (with misshapen cells) after prolonged incubation at elevated temperatures. Allelic to hpo1-1 (IA466)
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01455 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janC-1/janC-1; hpo1-2/hpo1-2 (janC-1; hpo1-2; janC, hpo1, IV)
Affected Genes: JANC (janusC), HPO1(hypoangular1)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: The sister clone (IA436,now extinct) manifests joint expression of the two mutations, which can be roughly summarized as the "...janus phenotype being superimposed on the hypoangular body plan".
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01576 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wag1[∆::neo3]/wag1[∆::neo3] (?)
Affected Genes: WAG1 (TTHERM_00299879)
Genomic Alteration: Micronucleus: Neo3 knockout of WAG1, heterozygous
Availability:
References:
Synonyms:
Notes: Heterozygous heterokaryon. WAG1 KO,
mates with Nol1 KO C11-9-A5-A4
Named NOL1 until found there was already a gene of that name. Re-named WAG1.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02704 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (JMJ2/jmj2-1[Δ21-672(::neo4, KanR2)]; pm-r, II)
Affected Genes: JMJ2 (TTHERM_00455500)
Genomic Alteration: Micronucleus: PAR1 gene partially replaced by neo4-KanR2 cassette. Macronucleus: JMJ2 gene partially replaced by neo4-KanR2 cassette.
Availability:
References:
Synonyms:
Notes: The knockout also introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01739 Copy
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