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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1::neo2/gtu1::neo2;Chx1/Chx1; Mpr?/Mpr? (MTT1/GTU1[HA]; gtu1::neo2/gtu1::neo2; pm-r, BTU1 has(E431,435D), BTU2 is replaced by bsr1, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 gene replaced by neo2 cassette Macronucleus: HA tagged GTU1 gene into the MTT1 coding region
Availability:
References:
Synonyms:
Notes: Progeny of GTUKO5 x GTUKO6 mating, rescued by biolistic transformation with pTTMG-HA ( GTU-HA fusion driven by MTT1 promoter at the MTT1 locus). Needs 0.1μg/ml to 0.5 μg/ml Cd to grow, depending on the growth medium. Can cross with cTTMG-HA#15 or #14
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01216 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaE-1/cdaE-1 (cdaE-1, V)
Affected Genes: CDAE (cell division arrest E). (Originally published as mo8).
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Incomplete fission-zone formation with arrest in late-cytokinesis, with the new oral apparatus often abnormal and frequently wedged within the arrested furrow (39°). Fairly high lethality in crosses. Clear-cut temperature-sensitive expression: no expression at 28°, penetrance high but not 100% at 39° C.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01457 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/ btu1-1[K350M] (BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 946bp immediately upstream of ATG--GFP coding--BTU1 3’; pm-r with Cd+2; tax-r, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 946 bp immediately upstream of ATG--GFP coding--BTU1 3’
Availability:
References:
Synonyms:
Notes: Complete somatic transformant of CU522 cells. Construct was pBBN-CDC2: BTU1 5’(600bp from400-1000bp upstream of BTU1 ATG)--Neo3--CDC2 5’flanking 946 bp immediately upstream of ATG--GFP coding- -1.4 Kb BTU1 3â€Â. BTU1 promoter was deleted.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02823 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI11/twi11[5’neo3,HAn];MPR1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: HA tagged TWI11
Availability:
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Synonyms:
Notes: heterozygote HA-tagged TWI11 heterokaryon. HA coding sequences was inserted just after the start codon of TWI11. Neo3 cassette was inserted to upstream of TWI11
mates with HA-TWI11 B1-2-3.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02797 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-2/mat1-2 (mat1-2, II)
Affected Genes:
Genomic Alteration:
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Notes: All three B2086 clones were used extensively for crosses in the Frankel lab in the late 1980's and 1990's.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01625 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
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Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01869 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (rpc5[3’neo3,HAc]; pm-r, VII)
Affected Genes: RPC5 (TTHERM_00094210)
Genomic Alteration: Macronucleus: HA tagged RPC5
Availability:
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Synonyms:
Notes: All Somatic copy of RPA5 was replaced with RPA5-HA construct, neo3, CU428background. RPA5 is the fifth largest subunit of pol I and III.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02718 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding
Availability:
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Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01909 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-2/cdaC-2 (cdaC-2, VII)
Affected Genes: CDAC (cell division arrest C) Originally published as mo3b
Genomic Alteration:
Availability:
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Synonyms:
Notes: Failure to complete cytokinesis accompanied by cell elongation (>36o C). Located on chromosome 5. Allelic to cdaC-1 (IA-119). Expresses 100% fission blockage at temperatures of 36o C. and higher.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01511 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaA-2/cdaA-2 (cdaA-2, III)
Affected Genes: CDAA (cell division arrest A) Originally published as mo1b
Genomic Alteration:
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Synonyms:
Notes: Partial failure to form a fission zone (39o C.) Fully penetrant at 39o C, but expressivity is far weaker than that of cdaA-1 at 39o, and instead resembles that of cdaA-1 at 35o C: dorsal ciliary rows remain continuous while ventral rows become subdivided, and a partial division furrow forms. Allelic to cdaA-1 (IA-104). The published phenotypic analysis was carried out on a different homozygous cdaA-2/cdaA-2 clone that is now extinct.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01510 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (CHX1; cy-s, II)
Affected Genes:
Genomic Alteration:
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Notes: Cycloheximide resistance functional heterokaryon.
Proper citation: RRID:TSC_SD00701 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; VPS13A/VPS13A (MPR1; VPS13A/vps13a-2::gfp; pm-r, mp-s, VII, VII)
Affected Genes: Vacuolar Protein Sorting 13A gene
Genomic Alteration:
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Synonyms:
Notes: Deposited line has been grown with high concentration of paromomycin (1 mg/ml) for phenotypic assortment, but it has not been subcloned. Gene has also been referred to as TtVPS13A. Stock has been maintained in SSP medium + 2 ug/ml CdCl2 + 1 mg/ml paromomycin.
Submitted by Lawrence Klobutcher, University of Connecticut Health Center
Proper citation: RRID:TSC_SD01757 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmD1/psmD-1 (psmD-1, III)
Affected Genes: PSMD (pseudomacrostome D)
Genomic Alteration:
Availability:
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Synonyms:
Notes: Pleiotropic: elongated phenotype with development of large oral-replacement or misplaced cell division oral primordia, especially in rich medium, at 39o C. Also frequent abnormal internal organization of oral structures, and fission arrest. The only known mutant allele of this gene, located on chromosome 3R. This is the most highly pleiotropic of the PSM mutant genes.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01517 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (ema1[∆?::neo3]; pm-r, VI)
Affected Genes: EMA1 (TTHERM_00088150)
Genomic Alteration: Macronucleus: Neo3 of EMA1
Availability:
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Synonyms:
Notes: Somatic KO of .DRH26, neo3, CU427 background
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02604 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (mp-r, VII)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It mates with gl∆H3/H4 #4 strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01362 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 0 bp with GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.; pm-r with Cd+2, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: CDC2 gene has the 1.6 kb just upstream of ATG lacking and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
Availability:
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Synonyms:
Notes: XS72 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Shortened CDC2 5’ flanking region is 0 bases but the CDC2 far 5’ flanking 1 kb sequence that is 1.6 b upstream of the ATG was used to do the homologous recombination. Neo3 cassette is in 3’ flanking region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02573 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, VI)
Affected Genes:
Genomic Alteration:
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Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00034 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 5 (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
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Notes:
Proper citation: RRID:TSC_SD01127 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A (neo in 3 flank) cotransformant., VI)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: KO of HHT1, HHT2 has mutation S10A
Availability:
References:
Synonyms:
Notes: Single cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01886 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP, ∆HHT3, ∆HHT4; pm-r,bsr-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2 Macronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Availability:
References:
Synonyms:
Notes: H3.2-GFP heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02614 Copy
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