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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Availability:
References:
Synonyms:
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02469 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cna1[∆::neo2]/cna1[∆::neo2] (?)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Micronucleus: Neo2 into CNA1
Availability:
References:
Synonyms:
Notes: Germline CenPA KO heterokaryon. CenPA coding is replaced by neo2. Star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01933 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: in B2086 (MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; Cna1p-GFPc, green centromeres, II)
Affected Genes: CNA1 (TTHERM_00146340), CenH3, CENP-A
Genomic Alteration: Macronucleus: GFP tagged CNA1 from the MTT1 locus
Availability:
References:
Synonyms:
Notes: Centromeric H3 protein, green by IF. Partial mac replacement of MTT1 gene with GFP c-terminal tagged CNA1 gene. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01812 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, T35E, S43E, S45E, T47E, T54E, T60K,I64K, H65K,T67K,T69K]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to glutamic acid (charge patch) and T60K, I64K, H65K, T67K, T69K
Availability:
References:
Synonyms:
Notes: Complete somatic tranformant of CU428 cells with pHHO5K /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines, neo 2 cassette in 3’ flanking region for selection. See paper.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02476 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 8/21/2002 from the pond designated CRWP in Ridgway, PA (lat. 41 25.052, long. -78 51.876, elevation 1613 ft). Used Geko GPS.
Geographical area: Allegheny National Forest
Collection number: 453
No micronucleus present.
Proper citation: RRID:TSC_SD01547 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[A1G] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-A1G.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02083 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (mp-r, II)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes
Availability:
References:
Synonyms:
Notes: G115B5xG204F2. HTA1, HTA2 double germline knockout heterokaryon. Can mate with G4A1F14A, G4B3C1A
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01391 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2] (htb1[∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 Macronucleus: KO of HTB1 with neo2
Availability:
References:
Synonyms:
Notes: ∆HTB1 knockout.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02082 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3]; ift172[∆::neo3]/ift172[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540), IFT172 (TTHERM_00089240)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A and of IFT172
Availability:
References:
Synonyms:
Notes: IFT122 and IFT172 double KO heterokaryon
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01790 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[duplicate ATU1 tail--AAA2]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AAA2 (duplicate ATU C-tail))
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with duplicate C-tail
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AAA2 duplicate ATU C-tail). See publications
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01834 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
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References:
Synonyms:
Notes: HA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02648 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: eri1::neo3/eri1::neo3; mpr1-1/mpr1-1 (eri1::neo3; pm-r, ?)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: ERI1 coding replaced by neo3 Macronucleus: ERI1 coding replaced by neo3
Availability:
References:
Synonyms:
Notes: homozygous Er1 ko homokaryon ‘a’ can be mating with homozygous Er1 ko homokaryon ‘d’; coding sequences were replaced by Neo 3. Developmental delay.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02777 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 3R (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deletion on right arm of chromosome 3.
Proper citation: RRID:TSC_SD00994 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Availability:
References:
Synonyms:
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with D3, H7, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02139 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Micronucleus: H4-neo replaces coding of BTU1, heterozygous
Availability:
References:
Synonyms:
Notes: Germline transformant (heterozygote). Transformed with BHAB-1 (BTU1 knockout construct)
Mates with B1KO-1
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01840 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[α-β hybrid tail, AAB2]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AAB2 (α-β hybrid ), ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with chimeric α-β hybrid tail.
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AAB2 (α-β hybrid ) See publications.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02021 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Availability:
References:
Synonyms:
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with D3, H7, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02140 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[∆::neo2]; MPR1; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Neo2 into HHO1 locus
Availability:
References:
Synonyms:
Notes: H1 knockout obtained by microinjection
mac transformant, parent is CU428.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02789 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaE-3/cdaE-3 (cdaE-3, II)
Affected Genes: CDAE (cell division arrest E)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Incomplete fission-zone formation with arrest in late-cytokinesis, with the new oral apparatus often abnormal and frequently wedged within the arrested furrow.
Expressed in many cells at normal growth temperatures, near 100% at 39°. Same phenotype as the allelic IA137 stock,but with stronger expression.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01458 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-2/janA-2; eja1-1/eja1-1 (janA-2; eja1-1; janA, eja1, II)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A mutation that causes the dorsal surface to be converted to a partial mirror-image of the ventral surface and blocks conjugation. Allelic to janA-1.
Proper citation: RRID:TSC_SD00643 Copy
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