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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HMGB/∆HMGB with neo (pm-r, mp-?)
Affected Genes: HMGB (TTHERM_00660180)
Genomic Alteration: Macronucleus: complete HMGB KO
Availability:
References:
Synonyms:
Notes: Through crossing of two homozygous HMGB gl KO heterokaryons.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01335 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1 (hht3[∆::neo2];hht4[∆::bsr1]; pm-r, bsr-r, ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1 Macronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
References:
Synonyms:
Notes: HHT3 HHT4 double knockout homozygotes homokaryon can mate with ∆hht3∆hht4-5
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02667 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 1 [chx1-1/chx1-1[B] or CHX1/CHX1[C3]] (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00375 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htay[∆123-388::neo3]/htay[∆123-388::neo3] (htay[∆123-388::neo3]; pm-r, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Micronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene Macronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene
Availability:
References:
Synonyms:
Notes: Knockout homokaryon of HTAY gene. Can mate with ∆HTAY(c2/3) 1-23.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02676 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01195 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, 3HAc)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pBTGHB HA rescued GTUKO 5x6 progeny. WT GTU with HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce.
Different single cell line than BTGHB #2
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02048 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-2 (III)
Affected Genes:
Genomic Alteration:
Availability:
References:
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Notes: A representative of the latest inbred generation of the B strain derived in the Frankel lab (by Leslie Jenkins). It was not used in any crosses with mutant clones.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01754 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; VPS13A/VPS13A (MPR1; vps13a-1::neo4; pm-r, mp-s, VII, VII)
Affected Genes: Vacuolar Protein Sorting 13A gene
Genomic Alteration: Macronucleus: knockout of VPS13A
Availability:
References:
Synonyms:
Notes: Deposited line is phenotypic assorted subclone with no detectable copies of wild type VPS13A gene. Gene has also been referred to as TtVPS13a. Stock has been maintained in SSP medium + 0.1 ug/ml CdCl2 + 6 mg/ml paromomycin.
Submitted by Lawrence Klobutcher, University of Connecticut Health Center
Proper citation: RRID:TSC_SD01758 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T146V)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pT146V-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 T146V gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus. Single cell (b)
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01364 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: N terminal HA tag in TWI 11 with neo3 homozygous (pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: N-terminal HA tag on Twi 11
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon HA -Twi 11. Mate with HA-Twi11 #4.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02066 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (HTB1/htb1[∆::neo2]; htb2 [∆::neo2] assorted to pm-s so must have lost neo2; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
Availability:
References:
Synonyms:
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02467 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2 (mp-r?, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 replaces coding of HTAX, HTA1, HTB1, HTB2
Availability:
References:
Synonyms:
Notes: HTX1/HTB1 quadruple KO heterokaryons;From mating of h∆∆HTA-F14A and h∆∆HTB-B3D3; made with B*VII. Not mating types II/VI/VII.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02627 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
Availability:
References:
Synonyms:
Notes: In mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region . Use the pTTMG construct (GTU coding driven by MTT promoter) to rescue GTU KO heterokaryon, is cadmium dependent. Use .25 to 1 ug/ml of CdCl2 to grow.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01416 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[HAc]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1-HA or neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with C-terminal HA tagged ATU1 gene.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02230 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous HMG C KO with BSR (mp-r)
Affected Genes: HMGB (TTHERM_00660180)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Obtained thru 1st round exclusion of ∆B∆C gl KO. Genotype confirmed by cross with 427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01387 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: act1[208::neo3]/act1[208::neo3] (pm-s , mp-?, ?)
Affected Genes: ACT1 (TTHERM_00190950)
Genomic Alteration: Micronucleus: ACT1 with neo3 inserted at Sfu1 site
Availability:
References:
Synonyms:
Notes: ACT1∆ KO homozygous in mic, heterokaryon
From the Gorovsky lab, University of Rochester..
Proper citation: RRID:TSC_SD01797 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[duplicate ATU1 tail--AAA1]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AAA1 (duplicate ATU C-tail))
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with duplicate C-tail
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AAA1, duplicate ATU C-tail). See publications
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01833 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[S29A] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with S29A
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-S29A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02097 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3]; pmr1-1; MAT2; cy-s pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01291 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (ngoA[5’neo3, ∆::ngoA promoter 104bp,GFP], VII)
Affected Genes: ngoA (TTHERM_00655890)
Genomic Alteration: Macronucleus: GFP into NgoA locus with 104 bp of NgoA promoter. and neo3 upstream
Availability:
References:
Synonyms:
Notes: XS 35 ngoA 5’-neo3-ngoA ch2p12-GFP-ngoA3’ Somatic transformant replacing WT ngoA gene with GFP coding driven by ngoA 5’ flanking 104 bp. Neo3 cassette is upstream of the 104 bp.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02703 Copy
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