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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes
Availability:
References:
Synonyms:
Notes: G115B5xG209C4. HTA1, HTA2 double germline knockout heterokaryon. Can mate with G4B1G6A, G4B1G10A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01390 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];pmr1-1; MAT2; cy-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01273 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BTU11-428,BLT1428-473))], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Availability:
References:
Synonyms:
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pbBc-1 construct. BTU1(AA 1-428) with BLT at Cterminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02243 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
Availability:
References:
Synonyms:
Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of T.WI11. neo3 cassette was inserted upstream of TWI11 coding.
mates with HA-TWI11 F2-1, F2-5
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02805 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BLT1/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Availability:
References:
Synonyms:
Notes: BLT germline heterokaryon, heterozygous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO,
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02252 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[T19A,T20A] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A, T20A
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-T(19/20)A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02099 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1, vacA/vacA (mpr1, vacA; mp-r, vac-, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01600 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Availability:
References:
Synonyms:
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with A5, H9, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02142 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (eri[∆?::neo3]; pm-r, ?)
Affected Genes: ERI2 (TTHERM_00585450)
Genomic Alteration: Macronucleus: ∆eri2 complete ko
Availability:
References:
Synonyms:
Notes: eri2 was expressed at low levels in growing, starved and conjugating cells. The coding sequences in MAC was replaced by Neo3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02024 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2, ∆, HAHAn(::PPO1)]; pm-r, VII)
Affected Genes: PPO1 (TTHERM_00865290, TTHERM_00865300)
Genomic Alteration: Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
Availability:
References:
Synonyms:
Notes: Used Xiayuan Song’s pXS 75 construct: neo 2 in 5 flank of MTT1 gene. 2 HA tags right after ATG. then PP50 coding sequence. PP50 is Ttherm. 00865300 into Ttherm 00865290 sequence. Need 100ug/ml pm in medium to keep the tagged gene present.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02821 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 7/13/2006 from the pond designated Gregg Lake in NH (latitude 43.04, longitude -71.99)
Presence of micronucleus not determined.
Proper citation: RRID:TSC_SD03083 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 4 (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01067 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Availability:
References:
Synonyms:
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01863 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E446A,E447A]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E446A, E447A and neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pAA derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E446A,E447A. No polyglycylation in this strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02559 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: DCR1/dcri[∆?::neo3] (DCR1; cy-r?, ?)
Affected Genes: DCR1 (TTHERM_01216140)
Genomic Alteration: Micronucleus: Nee3 cassette into DCR1 locus
Availability:
References:
Synonyms:
Notes: Heterozygous heterokaryon of DCR1 in B2086XCU427 background, can mate with SAI1 KO BXC7 0.6-15-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02834 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01471 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (hht1[∆::neo2]; hht1[∆::neo2]; HHF2; hht2[K9Q]; pm-r, ?)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: WT HHF2 and mutated HHT2 with K9Q
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 KO stains were mated and rescued with wt HHF2 gene/HHT2 gene with K9Q mutation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02042 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1[C3]-1/chx1[C3]-1 (CHX1[C3]; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00661 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1, vacA/vacA (mpr1, vacA; mp-r, vac-, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01599 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K130R]; pm-r, mp-r, II)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: neo2 replaces HTAZ coding Macronucleus: HTAZ coding either neo2 or mutated to K130R
Availability:
References:
Synonyms:
Notes: New batch, rescue of h∆HTZ1-G304A1 and G311A1.
This strain was listed as pm-r but is now pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02689 Copy
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