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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 2L (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
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Notes: Deletion on left arm of chromosome 2.
Proper citation: RRID:TSC_SD00878 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CnjB/cnjB[∆?::neo3] (cnjB[∆?::neo3]
CnjB:neo3, fully assorted; pm-r)
Affected Genes: cnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: Part of CnjB coding replaced with neo3 Macronucleus: Part of CnjB coding replaced with neo3, completely assorted
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Notes: somatic knock-outs of CnjB obtained by mating heterozygous CnjB KO heterokaryon with WT then assorted to complete KO in mac; mate with ∆CnjB #1 and ∆CnjB #6
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01846 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD01170 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (V)
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Notes: Collected by Paul Doerder on 6/1/2003 from the pond designated SG29 in PA (latitude 41.73, longitude -79.23)
Presence of micronucleus not determined.
Proper citation: RRID:TSC_SD03077 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD01174 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; cdaA/cdaA; Nulli 2L (CHX1; mpr1-1; cy-s, mp-r, tr, IV)
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Proper citation: RRID:TSC_SD00122 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1L (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
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Notes: Deletion on left arm of chromosome 1.
Proper citation: RRID:TSC_SD00763 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 3 [B or C3] (cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00367 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rmm4[C3]/rmm4[C3]; mpr1-1/mpr1-1 (pmr1; mpr1-1; pm-r, mp-r, VI)
Affected Genes:
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Notes: rmm = rDNA maturation or maintenance mutation, located in the rDNA
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01614 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
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Notes: Collected by Paul Doerder on 7/13/2006 from the pond designated Gregg Lake in NH (latitude 43.04, longitude -71.99)
Presence of micronucleus not determined. Mated with < 6 testers.
Proper citation: RRID:TSC_SD03082 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2,His-n]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
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Notes: His-tagged hv2 transformant. Checked by western blot. His-hv2 is expressed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02670 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (hht1[∆::neo2]; hht1[∆::neo2]; HHF2;
hht2[K9Q]; pm-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: WT HHF2 and mutated HHT2 with K9Q
Availability:
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Notes: Germline heteokaryon Major H3 and H4 KO stains were mated and rescued with wt HHF2 gene/HHT2 gene with K9Q mutation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02311 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1; cdaF-1/cdaF-1 (cdaC-1; cdaF-1; cdaC, cdaF, II)
Affected Genes: CDAC (cell division arrest C), CDAF (cell division arrest F)
Genomic Alteration:
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Notes: Joint expression of cdaC and cdaF patterns of fission arrest. The published phenotypic analysis was carried out in a sister clone that is no longer available.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01588 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1]
(hht3[∆::neo2];hht4[∆::bsr1]; pm-r, bsr-r, ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1 Macronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
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Notes: Homozygous homokaryon progeny of crossing ∆3N4B heterokaryons, C1nbe mated with ∆3N4B-2
This strain was stated to be pm-r, but proved not to be when thawed.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02557 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: DCR1/dcri[∆?::neo3] (DCR1; cy-r?, ?)
Affected Genes: DCR1 (TTHERM_01216140)
Genomic Alteration: Micronucleus: Nee3 cassette into DCR1 locus
Availability:
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Notes: Heterozygous heterokaryon of DCR1 in B2086XCU427 background, can mate with SAI1 KO BXC7 1.0-1-2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02833 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1]
(hht3[∆::neo2];hht4[∆::bsr1]; pm-r, bsr-r, ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1 Macronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon progeny of crossing ∆3N4B heterokaryons, C1nbe mated with ∆3N4B-6
This strain was stated to be pm-r, but proved not to be when thawed.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02556 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1 (Originally published as mo3a) (cdaC-1; cdaC, III)
Affected Genes: CDAC (cell division arrest C) (originally published as mo3)
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Notes: Located on chromosome 5. A sister clone of IA118, which expresses near complete fission blockage at temperatures of 39° C and 15°C (IA118 is reported in Dev. Biol. 58:255-275 but is extinct).
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01745 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmA-2/psmA-2 (psmA-2; psmA, II)
Affected Genes: PSMA (pseudomacrostomeA)
Genomic Alteration:
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Notes: A considerably more weakly expressed allele of psmA-1 (IA223) and psmA-3 (IA485, IA486). Delayed expression at 39° of a phenotype qualitatively similar to that of the "strong" alleles.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01747 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1; cdaD-2/cdaD-2 (cdaC-1; cdaD-2; cdaC, cdaD, IV)
Affected Genes: CDAC (cell division arrest C), CDAD (cell division arrest D)
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Notes: Enhancement of fission-arrest phenotype, with fission arrest commonly observed at 28oC, which is not observed in either of the single mutants.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01590 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (tcd2[5’neo4,HAHAn]; MPR1-1; pm-r, VII)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: Neo4 upstream and 2 HAtags inserted after the ATG of TCD2
Availability:
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Notes: HA-TCD2p localized only to crescent stage micronucleus
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02327 Copy
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