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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; ts103/ts103 (CHX1; cy-s, tr, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00140 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpD-2/mpD-2 (mpD-2, II)
Affected Genes: MPD (membranellar pattern D)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Formation of four or five well-formed membranelles in the oral apparatus at 39-40 degrees. Allelic to and phenotypically nearly identical to IA305 (mpD-1). Detailed phenotypic analysis was carried out on a different mpD-2/mpD-2 clone.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01231 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1
(mtt1-?[5’neo2,∆::BLT1, HAc], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’flanking region
Availability:
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Notes: CU428 somatic replacement with BLT-HA tag in the MTT1 locus, has Neo2 in 5’ flanking region of MTT1. Wild type BLT1 gene still present. This is a partial replacement. Cannot single-cell, only some cells express the HA tagged BLT when induced with Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02562 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: lkn1-2/lkn1-2 (lkn1-2, III)
Affected Genes: LKN1 (low kinety number 1)
Genomic Alteration:
Availability:
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Synonyms:
Notes: Reduced number of ciliary rows, accompanied by some irregularities in ciliary rows; cells tend to be short and squat. Expression is not temperature-sensitive. Allelic to lkn1-1(IA319): similar phenotype, fully penetrant but more weakly expressed. Fully penetrant at 29o C. Detailed phenotypic analysis was carried out on its sister clone, IA329.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01518 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 chimera protein hy3, H3(1-75) H3.3(76-90), H3(91-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2(TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Availability:
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Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein hy3, H3(1-75) H3.3(76-90), H3(91-135) rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02692 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ? (Partial replacement
CDC2/cdc2-? [3’ neo3, ∆,(::GFP)]; pm-r with Cd+2, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: CDC2 gene has the 942 CDC2 5’ flanking region that includes the H1 dephosphorylation enriched region and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
Availability:
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Notes: XS67 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Neo3 cassette is in 3’ flanking region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02572 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (WT; WT, VII)
Affected Genes:
Genomic Alteration: Macronucleus: RPL29::frame shift NEO
Availability:
References:
Synonyms:
Notes: NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03309 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 3L (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
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Notes: Deletion on left arm of chromosome 3.
Proper citation: RRID:TSC_SD00954 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (ory-r, V)
Affected Genes:
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K (homozygous)
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Notes:
Proper citation: RRID:TSC_SD00281 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAZ ; MTT1/mtt1[5’neo2,∆::HTAZ(K4R)]; pm-r, VII)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: inserted HTAZ with K4R Into MTT1 locus and neo2 in 5’flanking of MTT1
Availability:
References:
Synonyms:
Notes: Cell has wt H2A.Z in its locus, and a mutated H2A.Z RKKKKK copy in MTT1 locus, neo2 cassette is inserted at 5’ of MTT, complete replacement ?. Somatic transformation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02462 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[T19A,T20A] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A, T20A
Availability:
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Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-T(19/20)A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02100 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; chx2-1/chx2-1; mpr1-1/mpr1-1 (CHX1; CHX2; MPR1; cy-s, mp-s, III)
Affected Genes:
Genomic Alteration:
Availability:
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Notes:
Proper citation: RRID:TSC_SD00046 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445D,E446D]; pm-r, mp-?
)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445D, E446D and neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pD5D6 derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E445D,E446D. No polyglycylation in this strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02585 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4Q,K7Q,K10Q,K13Q,K16Q,K21R]; pm-r, ?)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4Q, K7Q, K10Q, K13Q, K16Q, K21R
Availability:
References:
Synonyms:
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated QQQQQR. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02073 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, II)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Availability:
References:
Synonyms:
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02471 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
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Notes:
Proper citation: RRID:TSC_SD00294 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1; GCP3/gcp3-1[Δ10-1201(::neo4, KanR2)] (MPR1/mpr1-1; GCP3/gcp3-1[Δ10-1201(::neo4, KanR2)]; mp-r, pm-r, ?)
Affected Genes: GCP3 (TTHERM_00112620)
Genomic Alteration: Micronucleus: GCP3 gene partially replaced by neo4-KanR2 cassette.
Availability:
References:
Synonyms:
Notes: The knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01782 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
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Proper citation: RRID:TSC_SD01146 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; mpr1-1/mpr1-1; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];mpr1-1; pmr1-1; MAT2; cy-s, mp-r, pm-r, ?)
Affected Genes:
Genomic Alteration:
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Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01269 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; cnj3-1/cnj3-1 (cnj3-1; pmr1-1; cnj3, pm-r, ?)
Affected Genes: CNJ3
Genomic Alteration:
Availability:
References:
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Notes:
Proper citation: RRID:TSC_SD00212 Copy
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