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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2,His-n]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: His-tagged hv2 transformant. Checked by western blot. His-hv2 is expressed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02670 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (hht1[∆::neo2]; hht1[∆::neo2]; HHF2;
hht2[K9Q]; pm-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: WT HHF2 and mutated HHT2 with K9Q
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 KO stains were mated and rescued with wt HHF2 gene/HHT2 gene with K9Q mutation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02311 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1; cdaF-1/cdaF-1 (cdaC-1; cdaF-1; cdaC, cdaF, II)
Affected Genes: CDAC (cell division arrest C), CDAF (cell division arrest F)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Joint expression of cdaC and cdaF patterns of fission arrest. The published phenotypic analysis was carried out in a sister clone that is no longer available.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01588 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1]
(hht3[∆::neo2];hht4[∆::bsr1]; pm-r, bsr-r, ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1 Macronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon progeny of crossing ∆3N4B heterokaryons, C1nbe mated with ∆3N4B-2
This strain was stated to be pm-r, but proved not to be when thawed.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02557 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: DCR1/dcri[∆?::neo3] (DCR1; cy-r?, ?)
Affected Genes: DCR1 (TTHERM_01216140)
Genomic Alteration: Micronucleus: Nee3 cassette into DCR1 locus
Availability:
References:
Synonyms:
Notes: Heterozygous heterokaryon of DCR1 in B2086XCU427 background, can mate with SAI1 KO BXC7 1.0-1-2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02833 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1]
(hht3[∆::neo2];hht4[∆::bsr1]; pm-r, bsr-r, ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1 Macronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon progeny of crossing ∆3N4B heterokaryons, C1nbe mated with ∆3N4B-6
This strain was stated to be pm-r, but proved not to be when thawed.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02556 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1 (Originally published as mo3a) (cdaC-1; cdaC, III)
Affected Genes: CDAC (cell division arrest C) (originally published as mo3)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Located on chromosome 5. A sister clone of IA118, which expresses near complete fission blockage at temperatures of 39° C and 15°C (IA118 is reported in Dev. Biol. 58:255-275 but is extinct).
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01745 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmA-2/psmA-2 (psmA-2; psmA, II)
Affected Genes: PSMA (pseudomacrostomeA)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A considerably more weakly expressed allele of psmA-1 (IA223) and psmA-3 (IA485, IA486). Delayed expression at 39° of a phenotype qualitatively similar to that of the "strong" alleles.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01747 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1; cdaD-2/cdaD-2 (cdaC-1; cdaD-2; cdaC, cdaD, IV)
Affected Genes: CDAC (cell division arrest C), CDAD (cell division arrest D)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Enhancement of fission-arrest phenotype, with fission arrest commonly observed at 28oC, which is not observed in either of the single mutants.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01590 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (tcd2[5’neo4,HAHAn]; MPR1-1; pm-r, VII)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: Neo4 upstream and 2 HAtags inserted after the ATG of TCD2
Availability:
References:
Synonyms:
Notes: HA-TCD2p localized only to crescent stage micronucleus
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02327 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1[C3]-1/pmr1[C3]-1 (PMR1[C3]; pm-s, I)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: C3 strain - functional heterokaryon carrying paromomycin resistance in micronucleus.
Proper citation: RRID:TSC_SD00663 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: exoB2/exoB2; gal1-1/gal1-1 (exoB2, gal1-1; exo-, gal-r, III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00666 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-4/cdaC-4 (cdaC-4, IV)
Affected Genes: CDAC (cell division arrest C) Originally published as mo3d
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Failure to complete cytokinesis accompanied by cell elongation (39o C). Allelic to cdaC-1 (IA-119) and cdaC-2 (IA-123). Generates 100% fission blockage at 39o C. but not at lower temperatures.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01512 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI1/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, heterozygous
Availability:
References:
Synonyms:
Notes: Heterozygous heterokaryon of TWI1 KO, can mate with ∆TWI1 hehe C4-4-3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02848 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA2 has neo2; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
Availability:
References:
Synonyms:
Notes: It is HTA1 WT somatic rescue strain of HTA1,HTA2 double knockout. Can mate with 1-3(2).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02170 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAZ/htaz[5’neo2,GFPc]; pm-r, VII)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: GFP tagged HTAZ
Availability:
References:
Synonyms:
Notes: Somatic transformation with pQR88, (GFP)n-hv1. Neo inserted at 5’flanking. Only a partial replacement-> lethal. Cell turns green altough not a complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02454 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1,K192A, R193A), HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A and C-terminal HA tag into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pGTU5 HA rescue of GTUKO 5x6 progeny, having mutation at K192A R193A in GTU1-1 gene.In the MTT1 locus. HA c-terminal tag.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02058 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcr2[∆?::neo3]/dcr2[∆?::neo3] (DCR2, ?)
Affected Genes: DCR2 (TTHERM_00316190)
Genomic Alteration: Micronucleus: homozygous KO of DCR2 with neo3
Availability:
References:
Synonyms:
Notes: Homozygous heterokaryon of ∆DCR2 Round I progeny of he∆SAI3 F2-2-pms-1.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02177 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, VII)
Affected Genes: PPO1 (TTHERM_00865290)
Genomic Alteration: Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
Availability:
References:
Synonyms:
Notes: Xiaoyuan’s HA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02733 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2]; mpr1-1/mpr1-1 (GTU1,mpr1-1; mp-r, VI)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 replaces coding of GTU1
Availability:
References:
Synonyms:
Notes: GTU germline heterokaryon, progeny from CU428 x B 2086 transformed with p∆GNII construct, can mate with CU428, same mating type as CU427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01368 Copy
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