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http://wiki.solariseclipse.net/PrimerDesigner
THIS RESOURCE IS NO LONGER IN SERVICE. Documented on August 19,2025. Software program to aid in designing of primers and creation of primer sheets. The program allows users to select a background and enter mutaions. An initial primer is then suggested. User can manipulate the selected primer to add or remove nucleotides from either 5? or 3? ends. A set of parameters reflecting the goodness of the primer is updated on the fly, as the user makes changes. Once happy with the primer, the information is saved in a primer sheet, which can then be uploaded to the BGME lab primer database on the Wiki.
Proper citation: Primer Designer (RRID:SCR_003189) Copy
http://sourceforge.net/projects/salt1/
Software that can accurately and sensitivity classify short reads of next-generation sequencing (NGS) into protein domain families. It is based on profile HMM and a supervised graph contribution algorithm. Compared to existing tools, it has high sensitivity and specificity in classifying short reads into their native domain families.
Proper citation: SALT (RRID:SCR_003187) Copy
http://sourceforge.net/projects/xorro-overlap/
Efficient paired-read overlap software program for use with Illumina sequencing.
Proper citation: XORRO (RRID:SCR_003181) Copy
http://compbio.cs.sfu.ca/software-novelseq
Software pipeline to detect novel sequence insertions using high throughput paired-end whole genome sequencing data.
Proper citation: NovelSeq (RRID:SCR_003136) Copy
http://mrcanavar.sourceforge.net/
Copy number caller that analyzes the whole-genome next-generation sequence mapping read depth to discover large segmental duplications and deletions. It also has the capability of predicting absolute copy numbers of genomic intervals.
Proper citation: mrCaNaVaR (RRID:SCR_003135) Copy
http://www.arnoldfoundation.org/
Private foundation whose funding activities are primarily centered around improving life in the United States. Its mission is to produce big and lasting changes in society over the long term. Their four areas of focus (2014) are: criminal justice, K-12 education, public accountability, and research integrity.
Proper citation: Laura and John Arnold Foundation (RRID:SCR_003240) Copy
http://sourceforge.net/projects/saskprimerfs/
Software pipeline for designing gene family specific PCR primers. It infers intronic regions of a target species and design for them by utilizing DNA sequence information from a reference organism.
Proper citation: SaskPrimerFS (RRID:SCR_003159) Copy
Commercial organization which provides reagents and services for molecular biology research. Its services include clone collections, microRNA solutions, genome editing, qPCR products, and fluorescent labeling and detection.
Proper citation: GeneCopoeia (RRID:SCR_003145) Copy
Web application providing online database and workspace for evolutionary research, specifically systematics (the science of determining the evolutionary relationships among species). It enables researchers to upload images and affiliate data with those images (labels, species names, etc.) and allows researchers to upload morphological data and affiliate it with phylogenetic matrices. MorphoBank is project-based, meaning a team of researchers can create a project and share the images and associated data exclusively with each other. When a paper associated with the project is published, the research team can make their data permanently available for view on MorphoBank where it is now archived.
Proper citation: MorphoBank (RRID:SCR_003213) Copy
https://github.com/brunonevado/Pipeliner
Software for evaluating the performance of bioinformatics pipelines for Next Generation re-Sequencing.
Proper citation: Pipeliner (RRID:SCR_003171) Copy
A private company offering a wide variety of Molecular Biology reagents, fluorescent proteins, luciferase assay substrates, genotyping kits, and various custom services. The company isalso in the RNAi field with its recent patents in Pol III promoter-driven siRNA, shRNA, and miRNA. They introduced high titer lentivirus, camelid antibodies, and cell based assays and also offer Baculovirus protein expression and Gryphon retrovirus systems.
Proper citation: Allele Biotechnology (RRID:SCR_003164) Copy
A professional association of historians, physicians, nurses, archivists, curators, librarians, and others that promotes and encourages research, study, writing, and interest in the history of medicine and allied fields.
Proper citation: American Association for the History of Medicine (RRID:SCR_003161) Copy
https://www.fishersci.com/us/en/brands/IAOCLVV5/fisher-bioreagents.html
An Antibody supplier
Proper citation: Fisher BioReagents (RRID:SCR_003374) Copy
http://www.jobtarget.com/corp/
JobTarget is the foundation upon which careers and companies are built. Our technology and services connect millions of job seekers to hundreds of thousands of companies through thousands of job boards. We facilitate faster, more meaningful connections by powering niche job sites that target specific audiences of talent, and providing technology and advertising solutions for employers to more quickly and cost-effectively locate the most qualified candidates for their openings. Explore our Products & Services to experience the JobTarget difference or check out Who We Serve to narrow in on the best solutions to serve you.
Proper citation: JobTarget (RRID:SCR_003365) Copy
https://github.com/ggloor/ALDEx2
Software tool to examine compositional high-throughput sequence data with Welch's t-test. A differential relative count abundance analysis for the comparison of two conditions. For example, single-organism and meta-rna-seq high-throughput sequencing assays, or of selected and unselected values from in-vitro sequence selections. Uses a Dirichlet-multinomial model to infer abundance from counts, that has been optimized for three or more experimental replicates. Infers sampling variation and calculates the expected Benjamini-Hochberg false discovery rate given the biological and sampling variation using several parametric and non-parametric tests. Can to glm and Kruskal-Wallace tests on one-way ANOVA style designs.
Proper citation: ALDEx2 (RRID:SCR_003364) Copy
http://cran.r-project.org/web/packages/RCircos/
Software package that provides a simple and flexible way to generate Circos 2D track plot images for genomic data visualization. The types of plots include: heatmap, histogram, lines, scatterplot, tiles and plot items for further decorations include connector, link (lines and ribbons), and text (gene) label. All functions require only R graphics package that comes with R base installation.
Proper citation: RCircos (RRID:SCR_003310) Copy
http://sourceforge.net/projects/primerdesigner/
High throughput PCR primer design software. Target regions defined through a rich set of descriptors, such as Ensembl accessions and arbitrary genomic coordinates, may be specified. Primer pairs are then selected computationally to produce a minimal amplicon set capable of tiling across the specified target regions. As part of the tiling process, primer pairs are computationally screened to meet the criteria for success with one of two PCR amplification protocols.
Proper citation: JCVI Primer Designer (RRID:SCR_003275) Copy
http://www.bioconductor.org/packages/release/bioc/html/ddCt.html
Software package providing an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. It requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The package implements a pipeline to collect, analyze and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.
Proper citation: ddCt (RRID:SCR_003396) Copy
http://www.bioconductor.org/packages/release/bioc/html/ggbio.html
An R package for extending the grammar of graphics for genomic data. The graphics are designed to answer common scientific questions, in particular those often asked of high throughput genomics data. All core Bioconductor data structures are supported, where appropriate. The package supports detailed views of particular genomic regions, as well as genome-wide overviews. Supported overviews include ideograms and grand linear views. High-level plots include sequence fragment length, edge-linked interval to data view, mismatch pileup, and several splicing summaries.
Proper citation: ggbio (RRID:SCR_003313) Copy
http://moma.dk/normfinder-software
Software for identifying the optimal normalization gene among a set of candidates. It ranks the set of candidate normalization genes according to their expression stability in a given sample set and given experimental design. It can analyze expression data obtained through any quantitative method e.g. real time RT-PCR and microarray based expression analysis. NormFinder.xla adds the NormFinder functionality directly to Excel. A version for R is also available.
Proper citation: NormFinder (RRID:SCR_003387) Copy
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