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https://geiselmed.dartmouth.edu/dartmouse/

Core dedicated to facilitation and development of congenic mice for pre clinical projects across United States. Provides genotyping, background checks to verify purity of mice DNA.

Proper citation: Darthmouth Geisel School of Medicine DartMouse Core Facility (RRID:SCR_017756) Copy   

•   Source: SciCrunch Registry

http://www.einstein.yu.edu/departments/genetics/resources/molecular-cytogenetics-core.aspx

Core provides tools for preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of entire genome. These tools include establishment of EBV transformed cell lines; isolation of DNA and mRNA from variety of tissue culture samples as well as primary biopsies; preparation of metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) and Spectral Karyotyping (SKY) or whole chromosome paints for human and mouse genome. Core personnel is trained to hybridize commercial probes and to designed locus specific probes for regions of interest to investigators. All probes are custom designed and in house generated.

Proper citation: Albert Einstein College of Medicine Molecular Cytogenetics Core Facility (RRID:SCR_017815) Copy   

•   Source: SciCrunch Registry

https://umiamihealth.org/sylvester-comprehensive-cancer-center/research/research-resources/shared-resources/flow-cytometry-shared-resource

Core provides analytical and preparative flow cytometers, to facilitate range of experiments in biology of both normal and cancer cells. Experimental analyses, including:Complex, 19 fluorescence parameter immuno-phenotyping;DNA content, ploidy and cell cycle analysis; 17 fluorescence parameter cell sorting and single-cell flow cloning; High speed (10,000 � 30,000+ cells per second) and efficiency (95%+ purity) cell sorting;Assessment of apoptosis;Molecular cytogenetics;Cell function studies;Micro-biological applications;Multiplexed and micro-particle-based analysis;Mass cytometry analysis and imaging;Fluidigm Helios plasma mass cytometer. Cells are labeled with antibodies conjugated to heavy metal markers, vaporized in plasma and detected with time-of-flight mass spectrometry;Fluidigm Hyperion solid tissue imager provides simultaneous imaging of labeled solid tissue sections, for mass cytometry analysis. FCSR has bank of metal reagent kits (for custom antibody conjugation), available for purchase at favorable rates compared to those available commercially. The bank has 35 unique labeling metals, for users to create custom reagents and panels. Please contact FCSR for further information and pricing. Robotic automated cell transfer A.L.S. CellCelector automated cell transfer robot. For automated transfer of single cells and colonies from culture plates, without pre-treatment. Integral bio-safety cabinet for BSL-2 cultures.

Proper citation: Miami University SCCC Flow Cytometry Core Facility (RRID:SCR_017817) Copy   

•   Source: SciCrunch Registry

http://neuralsci.org/neuracell

Core facility provides neural stem cells (NSC) and everything you need to grow them, custom lentiviral shRNA and over-expression vectors, consult based characterization service where we can assess how your products or reagents affect stem cell performance and behavior. Services include:Cell Supply,Adult SVZ Neural Stem Cells,Any Age Embryonic stem cells from any region of the brain,Human Retinal Pigmented Epithelial cells,RPE iPSCs,iPSCs,Neural Progenitor Cells (NPCs),Custom Orders,Media/ Dissociation Reagents,High-performance Media formulated according to optimal cell growth conditions,Complete Defined Media for Neural Stem Cell Culture,Complete Defined Media for Human Retinal Pigmented Epithelial Culture,Cell characterization. Performs assays to verify how stem cell populations behave in response to certain environments, plastics, reagents, etc., using trade secret assays via FACS and cell culture techniques. This can be used for neural stem cells as well as embryonic stem cells.Cell banking solutions shall ensure that their integrity is maintained, and that sufficient supply is readily available. We will also expand cells, harvest cells, generation the freezing medium and thaw cells to test performance. Provides Generation of custom lentiviral shRNA constructs,Generation of custom lentiviral over-expression constructs,Supply of NSC lines already expressing these shRNA or over-expression constructs.

Proper citation: Neural Stem Cell Institute NeuraCell Core Facility (RRID:SCR_017821) Copy   

•   Source: SciCrunch Registry

https://chemistry.as.miami.edu/research-facilities/x-ray-crystallography-resources/index.html

Core provides upgraded Bruker SMART 1000 CCD single crystal diffractometer to SMART APEX II CCD Area Detector, and its low-temperature LT3 device to KRYO-FLEX low-temperature device. Instrument which is equipped with Mo radiation source is used for small molecule crystal structure determination.

Proper citation: Miami University X-Ray Crystallography Resources Core Facility (RRID:SCR_017824) Copy   

•   Source: SciCrunch Registry

https://voices.uchicago.edu/cscenter/

Facility provides high throughput cell- and non-cell-based screening. Has number of validated cell lines and variety of small molecule or siRNA screening libraries available to investigators.

Proper citation: University of Chicago Cellular Screening Center Core Facility (RRID:SCR_017914) Copy   

•   Source: SciCrunch Registry

https://him.uchicago.edu/

Facility serves as specialized laboratory performing correlative assays for cancer-based clinical trials. Participates in project development to assist in assay selection and optimization prior to study initiation. Offers pick-up service for specimens from patients at U of C Hospital or outpatient clinics, and process samples as indicated for assays being performed. For some studies, isolation and freezing of peripheral blood lymphocytes for analysis at later date is performed, while for others immediate staining of whole blood for specific cell surface markers of interest is carried out. Prepares clinical grade products, such as peptide vaccines, for administration into patients.Assays performed include ELISA, ELISPOT, tetramer binding assays, detection of cell surface markers by flow cytometry, and biochemical assays such as Western Blots, RNA extraction from tumor biopsies and either real time RT-PCR for specific transcripts or preparation of samples for gene expression profiling. Facility is equipped with MACSQuant Flow Cytometer, ELISA microplate reader, ELISPOT reader, PCR thermocyclers, CO2 incubators, biosafety cabinets, centrifuges, and equipment for Western blot analysis. Performs assays on human samples but consideration will be given to expanding these services to mouse-model systems.

Proper citation: University of Chicago Human Immunologic Monitoring Core Facility (RRID:SCR_017916) Copy   

•   Source: SciCrunch Registry

https://www.une.edu/research/cobre/behavioral

Core provides technical expertise, training, instrumentation and related services for assessing behavior in animals to help to gain insight into function of nervous system and mechanisms of acute and chronic pain.Services include Behavioral Testing; General Behavioral Phenotyping: Observational Screens, Motor Function;Pain/Sensory Testing: Thermal, Mechanical, Chemical;Additional Neurobehavioral Tests: Psychiatric, Addiction, Learning and Memory;Miscellaneous Systems: GI Transit, Cardiovascular and Respiration.Other Services:Assistance choosing behavioral test and statistical tests for assessing results;Training in surgical techniques for small animal surgeries;Staff expertise includes surgical methods for producing pain models; dosing of drugs/anesthetics including central, systemic and localized administration; and tissue extractions;Animal care training.Genotyping Services is component of Behavioral Core that focuses on identifying allele state of genes found in transgenic mouse lines. Specializes in Hot-start PCR, Touchdown PCR, ARMs PCR, multiplex PCR and standard PCR protocols,Primer Design and Optimization.

Proper citation: New England University Behavioral and Genotyping Core Facility (RRID:SCR_017883) Copy   

•   Source: SciCrunch Registry

http://mmcri.org/ns/?page_id=221

Core provides viral vector services dedicated to development and production of viral vectors for angiogenesis, stem cell and regenerative medicine and clinical renal research. Viral vectors are produced in dedicated BL2 facility within MMCRI building. Vector systems currently being used include adenovirus, retrovirus and lentivirus. Services Available:Transfection and transduction of gene-specific viral DNA provided by principal investigators;Small, large and custom scale production of adenovirus;retrovirus and lentivirus (2nd and 3rd generation packaging);TCID50 titration of adenovirus;Titration of retrovirus and lentivirus (fluorescence marker or antibiotic resistance);Test for replication competent virus (RCV) in lentivirus preparations.

Proper citation: Maine Medical Center Research Institute Viral Vector Core Facility (RRID:SCR_017882) Copy   

•   Source: SciCrunch Registry

http://sites.northwestern.edu/pldcore/

Core provides means to grow thin films of metals and metal oxides. Facility contains two state of art deposition chambers PVD PLD/MBE 2300 and a PVD nanoPLD 1000. PLD provides convenient way to make thin films of materials heretofore studied in bulk by pressing and sintering target from bulk material; facilities are available at Northwestern for pressing and sintering of targets.

Proper citation: Northwestern University Pulsed Laser Deposition Core Facility (RRID:SCR_017889) Copy   

•   Source: SciCrunch Registry

https://cri.utsw.edu/facilities/mouse-genome-engineering-core/

Core provides production of mouse models to support fundamental and translational research. Provides transgenic, gene targeting,DNA microinjection services, CRISPR/Cas9 microinjection, ES cell manipulation and other microinjection services to generate transgenic mice, knock-in/knock-out mice and chimeric mice harboring select genetic mutations. Other services include mouse sperm cryopreservation and recovery, in vitro fertilization (IVF), rederivation of pathogen free mouse lines and derivation of mouse embryonic stem cell lines. Provides general consultations on experimental designs and vectors for gene modification-related projects, DNA preparation, recombinant ES clones, mouse genotyping, colony breeding and husbandry, customize services as requested to support development of animal models for modeling human diseases.

Proper citation: University of Texas Southwestern Medical Center Mouse Genome Engineering Core Facility (RRID:SCR_017921) Copy   

•   Source: SciCrunch Registry

https://www.brown.edu/research/facilities/flow-cytometry-and-sorting/home

Core provides technical assistance to Brown researchers by performing flow cytometry-based analysis and sorting. Facility has 4-laser, 19-parameter BD FACSAria IIIu for flow and sorting applications, as well as 3-laser, 15-parameter BD FACSCelesta equipped with high throughput system for analysis of samples from 96 well plates.

Proper citation: Brown University Flow Cytometry and Sorting Core Facility (RRID:SCR_017886) Copy   

•   Source: SciCrunch Registry

http://cells.nmh.org/

Core offers researchers, clinicians and biotech companies regulatory compliant labs and services to manufacture minimally manipulated and complex biotechnology products.

Proper citation: Northwestern University and Northwestern Memorial Health Mathews Center for Cellular Therapy Core Facility (RRID:SCR_017881) Copy   

•   Source: SciCrunch Registry

https://med.nyu.edu/research/scientific-cores-shared-resources/proteomics-laboratory

Core offers specialized expertise for analysis of proteins and peptides using mass spectrometry. Develops new methods and customized approaches for proteomic analysis and suggests experimental strategies and sample preparation prior to mass spectrometry analysis. Services include:comprehensive protein identification ,analysis of affinity purified complexes,characterizing protein post-translational modifications,de novo sequencing,label and label-free quantitation ,multiplexed quantitation global phosphorylation and ubiquitin analysis,analysis of laser-capture microdissected formalin-fixed paraffin-embedded tissue,secretome analysis,crosslinking analysis,disulfide mapping.

Proper citation: New York University School of Medicine Langone Health Proteomics Laboratory Core Facility (RRID:SCR_017926) Copy   

•   Source: SciCrunch Registry

https://isairr.bsd.uchicago.edu/

Core offers imaging modalities, techniques, and services for in vivo imaging of small animals and ex vivo imaging of tissue/organ specimens. iSAIRR sub-cores feature magnetic resonance imaging and spectroscopy (MRIS); optical imaging (bioluminescence and fluorescence); positron emission tomography, single photon emission computed tomography, and computed tomography (PET/SPECT/CT).Services include Assistance with experimental design,Assisted and/or independent image acquisition,Veterinary support for all imaging modalities,Assistance with data processing and interpretation.

Proper citation: Chicago University Integrated Small Animal Imaging Research Resource Core Facility (RRID:SCR_017923) Copy   

•   Source: SciCrunch Registry

https://med.nyu.edu/research/scientific-cores-shared-resources/genome-technology-center

Core provides range of services related to genome, epigenome, and transcriptome analysis. Offers technologies including Illumina deep sequencing and sample preparation for variety of applications, including DNA and RNA sequencing (DNA- and RNA-seq), exome sequencing, targeted capture, chromatin immunoprecipitation sequencing (ChIP-seq), methylation sequencing (Methyl-seq), metagenomics, and many others;Automation of Illumina library and targeted capture preps, including 16S ribosomal RNA (rRNA) sequencing;Oxford Nanopore sequencing (long reads);Bio-Rad Droplet Digita polymerase chain reaction (PCR);Nanostring nCounter;Single-cell RNA- and DNA-seq using the C1 Auto Prep System from Fluidigm, and 10x Genomics Chromium System.Provides expertise on strategies to achieve research goals in any field related to genomics, and can tailor bioinformatics analysis to individual project. If you supply us with nucleic acids, we can perform every step required to help you achieve your desired results.

Proper citation: New York University School of Medicine Langone Health Genome Technology Center Core Facility (RRID:SCR_017929) Copy   

•   Source: SciCrunch Registry

https://ki.mit.edu/sbc/nanowell

Nanowell Cytometry platform is shared resource housed in Flow Cytometry Core that provides Koch Institute and MIT researchers access to Microengraving and SeqWell technologies, developed in Love and Shalek Laboratories at Koch Institute. Microengraving platform enables measurement of multiple traits from individual cells derived from wide array of tissues or cell types, such as immune cells either in their native condition or upon stimulation. Platform allows simultaneous assessment from small or large numbers of cells. Additionally, it is possible to obtain genetic information from individual cells of interest. Platform has been employed in projects related to antibody discovery, tumor profiling from various cancer tissues, and immune cell functions in various allergic, infectious or auto-immune diseases.Core offers access to CellCelector, tool which enables users to isolate single cells using ultra-precise robotic micromanipulator. Offers access to nanowell microplates which enable automated isolation of monoclonal and viable clones. Seq-Well is portable, low-cost platform for massively-parallel single-cell RNA-Seq. Barcoded mRNA capture beads and single cells are sealed in array of subnanoliter wells using semi-permeable membrane, enabling efficient cell lysis and transcript capture.

Proper citation: Massachusetts Institute of Technology Koch Institute Flow Cytometry Core Facility (RRID:SCR_017895) Copy   

•   Source: SciCrunch Registry

https://med.nyu.edu/research/scientific-cores-shared-resources/genotyping-core-laboratory

Core provides fast, accurate, convenient, and affordable genotyping technologies. We use conventional polymerase chain reaction (PCR) and gel electrophoresis methods. By pooling samples from group of users, we can better ensure accuracy, cost-effectiveness, and quality control.

Proper citation: New York University School of Medicine Langone Health Genotyping Core Laboratory Core Facility (RRID:SCR_017932) Copy   

•   Source: SciCrunch Registry

http://massspec.chem.wisc.edu

Core provides mass spectrometers including Thermo Q Exactive Plus,Bruker impact II ,Bruker microflex LRF,Bruker ULTRAFLEX III,Shimadzu GCMS-QP2010S,Waters Acquity LCMS.

Proper citation: University of Wisconsin-Madison Chemistry Instrumentation Center - Mass Spectrometry Core Facility (RRID:SCR_017931) Copy   

•   Source: SciCrunch Registry

https://med.nyu.edu/research/scientific-cores-shared-resources/center-biospecimen-research-development

Core mission is to drive scientific discovery through use of human specimens.Helps facilitate translational research, match human specimen resources to scientific needs, foster multi-investigator collaborative projects, and catalyze scientific innovation,improving researcher access to existing biospecimen resources, and enhancing institution-wide tissue-banking efforts and capacity. Another priority is to implement campus-wide policy for human biospecimen collection and storage to ensure regulatory compliance.

Proper citation: New York University School of Medicine Langone Health Center for Biospecimen Research and Development Core Facility (RRID:SCR_017930) Copy   

•   Source: SciCrunch Registry