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Species: Xenopus laevis
Genetic Insert: casein kinase 1, epsilon
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: From John Graff, in Peters et al 1999.
Notes for Use: Select clones with amp.
Reference: used in Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_16725 Copy
Species: Xenopus laevis
Genetic Insert: beta-catenin-myc
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid History:
Constructed by David on date of 2/9/95 in book XVI p.106.
Notes for Use:
Full-length Xenopus beta-catenin reconstructed by taking the Cla-SphI vector fragment from XBC20 and the SphI-Cla sequences from XBC24 (encoding the C-terminus of beta-catenin). This corrects possible problems with the C-terminus os XBC20. To make RNA, cut with NotI and transcribe with SP6.
Ref: Yost et al. Genes and Development 10:1443-1454, 1996.
Proper citation: RRID:Addgene_16842 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Transcription: linearize with Not I and transcribe with SP6.
Plasmid History:
Constructed by Randall T. Moon.
Notes for Use:
Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.
Proper citation: RRID:Addgene_16788 Copy
Species: Xenopus laevis
Genetic Insert: DONSON
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_208799 Copy
Species: Xenopus laevis
Genetic Insert: Cdc45
Vector Backbone Description: Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Simon AC, Sannino V, Costanzo V, Pellegrini L. Structure of human Cdc45 and implications for CMG helicase function. Nat Commun. 2016 May 18;7:11638. doi: 10.1038/ncomms11638. PMID: 27189187; PMCID: PMC4873980.
Proper citation: RRID:Addgene_208796 Copy
Species: Xenopus laevis
Genetic Insert: DONSON
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_208803 Copy
Species: Xenopus laevis
Genetic Insert: DONSON
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_208801 Copy
Species: Xenopus laevis
Genetic Insert: Xl-H3∆Nterm[R42]
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4640; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_221201 Copy
Species: Xenopus laevis
Genetic Insert: hnRNPAB X2-RBD
Vector Backbone Description: Backbone Size:6457; Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.10.566489v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216516 Copy
Species: Xenopus laevis
Genetic Insert: hnRNPAB X2-IDR
Vector Backbone Description: Backbone Size:6457; Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.10.566489v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216517 Copy
Species: Xenopus laevis
Genetic Insert: hnRNPAB X2 FL
Vector Backbone Description: Backbone Size:6457; Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.11.10.566489v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216515 Copy
Species: Xenopus laevis
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RISAP; Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: N-terminal deleted Xenopus beta-catenin that acts as a stable constitutively active variant.
Proper citation: RRID:Addgene_14041 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' and 3' UTR
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Derivative of pSP64T that includes BglII, EcoRV, and SpeI insertion sites and stop codons for all three reading frames downstream of the cloning sites. See author's map for more information.
Proper citation: RRID:Addgene_15034 Copy
Species: Xenopus laevis
Genetic Insert: islet1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15015 Copy
Species: Xenopus laevis
Genetic Insert: Mixer
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS300 (modified pCS2); Vector Types:Other, xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: pCS300 differs from pCS2 in that the polylinker II Not I site was moved to the polylinker I area and an Asc I and Pme I site were added to the polylinker II area.
You can use the CMV promoter in tissue culture cells or Xenopus embryos.
See the author's map for more cloning information. See the pdf attachment for information about making antisense probes.
Proper citation: RRID:Addgene_15038 Copy
Species: Xenopus laevis
Genetic Insert: Activin Beta b
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: See author's map for more information.
Proper citation: RRID:Addgene_15045 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-11
Vector Backbone Description: Backbone Size:3260; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert includes 360 bp of 5' UTR and 750 bp of 3' UTR. See author's map for more information.
Proper citation: RRID:Addgene_15043 Copy
Species: Xenopus laevis
Genetic Insert: BMP2-Vg1 dom neg
Vector Backbone Description: Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Other, Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
References:
Comments: A dominant negative mutant version of pSP64T3 BMP2 pro Vg1.
See article for more cloning details.
The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.
Proper citation: RRID:Addgene_15074 Copy
Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pMV2, derivative of pMT3, is a mammalian expression vector. This construct conatins an EcoRI-SalI insert of the p64TNE BMPR clone with no untranslated region. The vector has an SV40 origin and an Ad MLP.
Proper citation: RRID:Addgene_15069 Copy
Species: Xenopus laevis
Genetic Insert: Vg1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2400; Vector Backbone:pSP70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: BamHI to EcoRI fragment of T-Vg1-A. See author's map for additional information.
Proper citation: RRID:Addgene_15070 Copy
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