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Species: Xenopus laevis
Genetic Insert: Xwnt-8 RNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: EcoR1 insert blunted into Bgl II blunt of SP64T.
Transcription: linearize with Bam H1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian on date of 12/90.
Citation: Christian et al., Development 111, 1045 (1991)
Proper citation: RRID:Addgene_16869 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-11
Vector Backbone Description: Backbone Size:0; Vector Backbone:pT7TS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: full length coding seq only, by PCR of lambda clones isolated by Purcell.
Insert size: 1 Kb.
Transciption: linearize with Sal1, Xba1, or BamH1 and transcribe with T7.
Plasmid History:
Constructed by Susan Purcell on date of 5/94.
Citation: Du et al., Molec. Cell Bio., 15, 2625 (1995)
Ku and Melton, Development, 119, 1161 (1993)
Proper citation: RRID:Addgene_16861 Copy
Species: Xenopus laevis
Genetic Insert: Xbhh-C
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expression ConstructXenopus Banded Hedgehog.
(a.k.a. Xbhh; hh1)
Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.
References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)
Subclone Xbhh-N in pT7Ts.
Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7.
Subclone Xbhh-dominant negative-C in pT7TS:
Encodes a leader sequence, a few amino acids amino terminal to the autoproteolytic site, and the carboxy-terminal domain. Linearize with BamH1 and transcribe with T7.
Proper citation: RRID:Addgene_16995 Copy
Species: Xenopus laevis
Genetic Insert: Xbhh
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expression Construct Xenopus Banded Hedgehog (a.k.a. Xbhh; hh1)
Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.
References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)
Proper citation: RRID:Addgene_16993 Copy
Species: Xenopus laevis
Genetic Insert: NES beta-catenin GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: NES of rabbit pkI at NH2 terminus of Beta-catenin GFP.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16837 Copy
Species: Xenopus laevis
Genetic Insert: TM beta-catenin 1-myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16834 Copy
Species: Xenopus laevis
Genetic Insert: beta-catenin GFP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: bGFP of Hiem et al., in Cla I site, with stop codon at 3' end. No myc epitope.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16839 Copy
Species: Xenopus laevis
Genetic Insert: XDpr-pt mutant
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Full length Xenopus Dapper (clone XE151), with last four amino acids mutated to MNTV instead of MMTV in the PDZ binding domain, in pCS2+. Xba1 site is NOT methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16768 Copy
Species: Xenopus laevis
Genetic Insert: delta LZ-XDpr-delta PDZB
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: 5'- GFP tag on XDpr that is doubly truncated to delete the first 129 amino acids and the last four amino acids; in pCS2+. Xba1 site is not methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16763 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: HA tagged Xenopus Dishevelled with a triple point mutation outside of the PDZ binding domain, that does not block binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461
Proper citation: RRID:Addgene_16779 Copy
Species: Xenopus laevis
Genetic Insert: GFP-XDpr
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: 5' GFP tag on full length Xenopus Dapper (clone XE151) in pCS2+. Xba1 site is DAM methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16770 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-PDZ
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996)
was engineered to now contain Dsh amino acids 1-267 and 381-736 (deleting 268-380).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16786 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-COOH
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-588 (deleting 599-736).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16784 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: HA tagged Xenopus Dishevelled with a point mutation in the binding pocket of the PDZ binding domain, that blocks binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16780 Copy
Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pGAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Dpr yeast clone in original prey vector. 1.6kb EcoR1 fragment corresponds to C-terminal 1.2 kb of Xenopus Dpr and 3' UTR in pGAD vector.
Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_17191 Copy
Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Coding sequence of original XDpr prey clone (M35) cloned into pGEX vector as a 1.2 kb EcoR1-Xba1 fragment (Xba site is DAM methylated).
Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_17192 Copy
Species: Xenopus laevis
Genetic Insert: XPKC-Alpha
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGem2; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: SmaI linearize, SP6 for sense RNA.
Notes for Use:
Ref: used in Otte and Moon, Cell 68:1021-1029, 1992.
Proper citation: RRID:Addgene_16746 Copy
Species: Xenopus laevis
Genetic Insert: XPKC-alpha-myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: Xenopus PKC alpha with a c-myc epitope at carboxy terminus. For transcription cut with Not 1 and use SP6.
Notes for Use:
Ref: Sheldahl et al., Current Biology 9:695-698, 1999.
Proper citation: RRID:Addgene_16745 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: cDNA from Melton lambda gt10 oocyte library.
Transcription: linearize with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Randall Moon on date of 9/15/90.
Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.
Citation: Moon et al., Development 119, 97 (1993)
Proper citation: RRID:Addgene_16985 Copy
Species: Xenopus laevis
Genetic Insert: siamois promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid History:
Constructed by David Kimelman, 6/6/96 XVII p. 98.
Notes for Use:
The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating.
The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate.
Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.
Proper citation: RRID:Addgene_17157 Copy
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