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Species: Synthetic
Genetic Insert: SynTF_VP64
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are generally reserved for c-terminal coding sequences in the MTK. Coding sequence from DOI:https://doi.org/10.1016/j.cell.2018.11.002 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123955 Copy
Species: Synthetic
Genetic Insert: hThal5.10-2_hg18 landing pad destination vector
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Type 0 parts are generally destination vectors in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123946 Copy
Species: Synthetic
Genetic Insert: TUL4R5 spacer
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Type 0 parts are generally destination vectors in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123937 Copy
Species: Synthetic
Genetic Insert: TUL6R7 spacer
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Type 0 parts are generally destination vectors in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123939 Copy
Species: Synthetic
Genetic Insert: TUL5R6 spacer
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Type 0 parts are generally destination vectors in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123938 Copy
Species: Synthetic
Genetic Insert: PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
Vector Backbone Description: Vector Backbone:MTK0_043; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Plasmid used to generate Fig. 5 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_124050 Copy
Species: Synthetic
Genetic Insert: PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
Vector Backbone Description: Vector Backbone:MTK0_043; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Plasmid used to generate Fig .4c,d Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_124042 Copy
Species: Synthetic
Genetic Insert: PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
Vector Backbone Description: Vector Backbone:MTK0_043; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Plasmid used to generate Fig. 5 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_124048 Copy
Species: Synthetic
Genetic Insert: Orange CaMBI 300
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor sequence for this section of the plasmid.
Proper citation: RRID:Addgene_124095 Copy
Species: Synthetic
Genetic Insert: Orange CaMBI 110
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The C-terminal copy of the CyOFP coding sequence is codon diversified to facilitate molecular biology procedures on Orange CaMBI and reduce the possibility of recombination between the two copies of the CyOFP coding sequence.
Proper citation: RRID:Addgene_124094 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:2541; Vector Backbone:Original; Vector Types:Mouse Targeting, Other, Human Targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124062 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24; Backbone Size:5162; Vector Backbone:pAAV-UbC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124102 Copy
Species: Synthetic
Genetic Insert: Membrane scaffold protein
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124275 Copy
Species: Synthetic
Genetic Insert: empty backbone (control)
Vector Backbone Description: Vector Backbone:pLG166; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124368 Copy
Species: Synthetic
Genetic Insert: SpyDock
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124618 Copy
Species: Synthetic
Genetic Insert: cggR_R250A
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124585 Copy
Species: Synthetic
Genetic Insert: cggR
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124584 Copy
Species: Synthetic
Genetic Insert: SpyCatcher002-oDi
Vector Backbone Description: Backbone Size:3648; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124661 Copy
Species: Synthetic
Genetic Insert: SpyCatcher002-oTri
Vector Backbone Description: Backbone Size:3648; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124662 Copy
Species: Synthetic
Genetic Insert: jRGECO1a
Vector Backbone Description: Backbone Size:9800; Vector Backbone:fCAG-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124775 Copy
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