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Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21240 Copy
Species: Mus musculus
Genetic Insert: TNF receptor-associated factor 6
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21624 Copy
Species: Mus musculus
Genetic Insert: Mef2c, Tbx5 and Gata4
Vector Backbone Description: Backbone Size:5871; Vector Backbone:pMx; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111810 Copy
Species: Mus musculus
Genetic Insert: integrin beta 1
Vector Backbone Description: Backbone Size:1380; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30153 Copy
Species: Homo sapiens
Genetic Insert: SIRT6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
Proper citation: RRID:Addgene_13817 Copy
Species: Mus musculus
Genetic Insert: Neurotrophin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_39981 Copy
Species: Homo sapiens
Genetic Insert: PDHA1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 487; Emission = 509. Localization data: Mitochondria
Proper citation: RRID:Addgene_54224 Copy
Species: Mus musculus
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCS2+ NLSMT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
Proper citation: RRID:Addgene_17440 Copy
Species: Mus musculus
Genetic Insert: VE-cadherin Tension Sensor
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:6265; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45848 Copy
Species: Mus musculus
Genetic Insert: VE-cadherin
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:6265; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45849 Copy
Species: Homo sapiens
Genetic Insert: Human H4
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42633 Copy
Species: Mus musculus
Genetic Insert: ATG5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA,
Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
Proper citation: RRID:Addgene_13095 Copy
Species: Mus musculus
Genetic Insert: Stat3 Y705F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Diagnostic Restriction Digest with BamHI (3.2kb, 2.8kb, and 2.0kb). Due to a stop codon error made when tagging the Stat3 clone with FLAG (DYKDDDDK) additional residues follow the tag (GPYSIVSPKC STOP). The ApaI cloning site is GGG CCC, which encodes the G and P residues coming after the FLAG tag. Therefore, if the insert is removed with a NotI and ApaI digestion, it will not have a stop codon included.
* E16K mutation found during QC
Proper citation: RRID:Addgene_8709 Copy
Species: Homo sapiens
Genetic Insert: NEAT1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4000; Vector Backbone:PCRII; Vector Types:Other, general cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: *To create this plasmid, hNEAT1 was amplified from HeLa cDNA. The insert contains two mismatches (C->T at bp 78, and A->G at bp 91, an A->G at bp 1666, and a deletion of TA at bp 2031 and 2032 using the numbering of NR_028272.1) compared to the canonical hNEAT1 sequence. These variants are likely to be encoded in the HeLa genome, as they appeared in multiple clones, but this is yet to be verified by sequencing of HeLa genomic DNA.
Proper citation: RRID:Addgene_61518 Copy
Species: Mus musculus
Genetic Insert: Mdm2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32365 Copy
Species: Mus musculus
Genetic Insert: Olig2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7791; Vector Backbone:pLVX-Tight-Puro; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_64842 Copy
Species: Mus musculus
Genetic Insert: epidermal growth factor receptor
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6280; Vector Backbone:pFLAG-CMV3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The EGFR internal signal sequence was removed during the cloning of this construct. When compared to GenBank reference sequence NP_997538.1, the signal sequence corresponds to amino acids 1-24
Proper citation: RRID:Addgene_18788 Copy
Species: Mus musculus
Genetic Insert: TRPM7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Other, Tetracycline Inducible; Bacterial Resistance:Ampicillin
References:
Comments: For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.
Proper citation: RRID:Addgene_45482 Copy
Species: Mus musculus
Genetic Insert: Mouse Protein Kinase C zeta
Vector Backbone Description: Backbone Size:11500; Vector Backbone:pMTH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_8414 Copy
Species: Homo sapiens
Genetic Insert: CD44 standard
Vector Backbone Description: Backbone Marker:Millenium Pharmaceuticals; Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Human CD44 cDNA,standard form no variable exons.
CD44 protein is stably expressed in MCF7Ras or HMEC-T cells but only transiently in BPEC-T cells. Use pBabe-CD44S for BPEC-T cells.
Proper citation: RRID:Addgene_19126 Copy
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