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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCDF1-mAtf4-HT-puro Resource Report Resource Website |
RRID:Addgene_101795 | Activating transcription factor 4 | Mus musculus | Ampicillin | PMID: | Backbone Marker:Systems Biosciences Inc; Backbone Size:6592; Vector Backbone:pCDF1-MCS2-EF1-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:10 | 0 | ||
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pGL3-mFgf21pro1-luc Resource Report Resource Website |
RRID:Addgene_101797 | Fibroblast growth factor 21 promoter | Mus musculus | Ampicillin | PMID:26487695 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:10 | 0 | ||
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pCSN061 Resource Report Resource Website |
RRID:Addgene_101725 | S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. | Ampicillin | PMID:28886218 | Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 0 | |||
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pAAV hSyn1 CaRhAC T2A tDimer Resource Report Resource Website |
RRID:Addgene_101722 | CatRhAC | Other | Ampicillin | PMID:29799525 | Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | E497K,C569D | 2022-04-22 03:15:08 | 0 | |
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pAAV hSyn1 CaRhGC wt T2A tDimer Resource Report Resource Website |
RRID:Addgene_101720 | CatRhGC | Other | Ampicillin | PMID:29799525 | Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:08 | 0 | ||
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AICSDP-22:CETN2-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_101781 | CETN2 Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:15:09 | 0 |
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AICSDP-28:TUBA1B-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_101785 | TUBA1B Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:15:10 | 0 |
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pRN1120 Resource Report Resource Website |
RRID:Addgene_101750 | Ampicillin | PMID:28886218 | Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 0 | ||||
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pGL3-Sox2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101761 | SRY-box 2 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 1 | ||
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pGL3-TGFb1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101762 | transforming growth factor beta 1 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 2 | ||
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CS2 Flag-Smad2 (3A) Resource Report Resource Website |
RRID:Addgene_101763 | SMAD2 | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Three Ser to Ala mutations in the phosphorylation sites (S464A, S465A, S467A) | 2022-04-22 03:15:09 | 0 | |
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pCB1532B-RFP Resource Report Resource Website |
RRID:Addgene_101854 | Ampicillin | PMID: | Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html | Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4378; Vector Backbone:pCB1532; Vector Types:Other, Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:11 | 0 | |||
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U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE Resource Report Resource Website |
RRID:Addgene_101852 | Ascl1, Brn2 | Mus musculus | Ampicillin | PMID:28646119 | Backbone Size:5845; Vector Backbone:pUC19; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:11 | 0 | ||
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pKAM-E-Flag-BFP Resource Report Resource Website |
RRID:Addgene_101867 | Evi-1 | Mus musculus | Ampicillin | PMID: | Vector Backbone:pLV-EF1a-PGK-BFPT2aPuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:11 | 0 | ||
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pcDNA3.1-ME-Flag Resource Report Resource Website |
RRID:Addgene_101860 | Mds1-Evi-1 | Mus musculus | Ampicillin | PMID: | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:11 | 0 | ||
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pFastBac1_FLAG-BPTF Resource Report Resource Website |
RRID:Addgene_102242 | Flag-BPTF | Homo sapiens | Ampicillin | PMID: | BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6]. | Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | |
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5TEY (METTL14) Resource Report Resource Website |
RRID:Addgene_101891 | METTL14 | Homo sapiens | Ampicillin | PMID: | N terminal tag: none. SGC Clone Sample ID: METTL14:JMC097-G06:C232534. SGC or PDG link: http://www.thesgc.org/structures/5TEY/ | Vector Backbone:pFBOH-LIC notag; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | |
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5TEY (METTL3) Resource Report Resource Website 1+ mentions |
RRID:Addgene_101892 | METTL3 | Homo sapiens | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL3:JMC094-C09:C231635. SGC or PDG link: http://www.thesgc.org/structures/5TEY/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 1 | |
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Bassik lab Human CRISPR-Cas9 Deletion Library - Proteostasis Resource Report Resource Website |
RRID:Addgene_101930 | Ampicillin | PMID:28474669 | Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | ||||
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Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility Resource Report Resource Website |
RRID:Addgene_101931 | Ampicillin | PMID:28474669 | Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 |
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