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Species: Other
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: Note that the TRIM5 insert in this plasmid contains a V434A polymorphism compared to GenBank ID AAW72440.1. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_79031 Copy
Species: Homo sapiens
Genetic Insert: OSF-TRIM5alpha(human)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_79034 Copy
Species: Other
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_79030 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Other, Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
References:
Comments: Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
Proper citation: RRID:Addgene_78501 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Other, Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
References:
Comments: Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
Proper citation: RRID:Addgene_78500 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Other, Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
References:
Comments: The new synthetic polylinker (SmaI site flanked by NotI) allows access to restriction sites within a cloned fragment to allow insertion of cassettes etc. Blue/White selection works.
Proper citation: RRID:Addgene_78502 Copy
Species: Homo sapiens
Genetic Insert: TRF2
Vector Backbone Description: Backbone Size:4560; Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_185443 Copy
Species: Other
Genetic Insert: OSF-TRIM5Cyp (owl monkey)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_79035 Copy
Species: Other
Genetic Insert: rhesus TRIM5alha-PFOS
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: The depositor-provided reference sequence shows M221T and P307T compared to DQ842021.1
Proper citation: RRID:Addgene_79038 Copy
Species: Homo sapiens
Genetic Insert: OSF-TRIM5alpha(human R332P)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_79037 Copy
Species: Homo sapiens
Genetic Insert: GATA Binding Protein 6
Vector Backbone Description: Backbone Marker:Addgene#25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRC2; Vector Types:Bacterial Expression, Other, entry vector for gateway cloning; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_72922 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Other, entry vector for gateway cloning; Bacterial Resistance:Gentamicin
References:
Comments: Gateway entry vector for expression of 3xFLAG LacZ
Proper citation: RRID:Addgene_83093 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pJQ200; Vector Types:Other, Suicide plasmid; Bacterial Resistance:Gentamicin
References:
Comments: This is a suicide vector for gene replacement. It is identical to pJQ200SK except that the multiple cloning site is from pBluescriptKS instead of from pBluescriptSK
Proper citation: RRID:Addgene_78498 Copy
Species: Homo sapiens
Genetic Insert: ID2
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2; Vector Types:Other, entry vector for gateway cloning; Bacterial Resistance:Gentamicin
References:
Comments: Gateway entry vector for expression of 3xFLAG ID2 (human)
Proper citation: RRID:Addgene_98390 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2; Vector Types:Other, entry vector for gateway cloning; Bacterial Resistance:Gentamicin
References:
Comments: Gateway entry vector for expression of 3xFLAG Luciferase
Proper citation: RRID:Addgene_98391 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2; Vector Types:Other, entry vector for gateway cloning; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_83274 Copy
Species:
Genetic Insert: rfp
Vector Backbone Description: Vector Backbone:pRIM2; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_74073 Copy
Species:
Genetic Insert: NA
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Gentamicin
References:
Comments: If LacI is added to the cell (e.g., in a plasmid), the expression of RFP will be somewhat repressed (unless if IPTG is added), as there is a lacO between the promoter and the gene's ATG. Attempts to remove this lacO were unsuccessful - we suspect the higher RFP expression might lead to cell death (in the cloning strain, E. coli DH5alpha).
Please note this strain has a higher %GC than E. coli, so PCRing (and sequencing) may be difficult.
Proper citation: RRID:Addgene_79642 Copy
Species: Synthetic
Genetic Insert: aacC1
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-54, 1995; Vector Backbone:pBSL15; Vector Types:Other, Bacterial vector based on pMB1 replicon; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_74106 Copy
Species: Homo sapiens
Genetic Insert: KIF5A (∆exon27)
Vector Backbone Description: Backbone Marker:GENEVA Biotech; Backbone Size:5944; Vector Backbone:pACEBac1, pIDS; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_184832 Copy
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