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Species: Other
Genetic Insert: WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Other, Fragment; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216074 Copy
Species: Other
Genetic Insert: gRNA expression with modified trRNA to recruit activators via PP7 tags
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Assembled Vector; Bacterial Resistance:Ampicillin
References:
Comments: pXPR_502 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216106 Copy
Species: Other
Genetic Insert: P2A_v1.1; NeoR_v1.1; Stop
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Other, Fragment; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216066 Copy
Species: Other
Genetic Insert: P2A_v1.1; TagRFP657_v1.1; Stop
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Other, Fragment; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216065 Copy
Species: Other
Genetic Insert: Cas9 [Sp]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Assembled Vector; Bacterial Resistance:Ampicillin
References:
Comments: pLentiCas9 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216104 Copy
Species: Other
Genetic Insert: ITRs from AAV2
Vector Backbone Description: Vector Backbone:pRDA_889; Vector Types:Mammalian Expression, AAV, Other, Destination; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216080 Copy
Species: Other
Genetic Insert: for adding plate and well barcodes via 6-9 and 9-10 modules
Vector Backbone Description: Vector Backbone:pRDA_792; Vector Types:Mammalian Expression, Lentiviral, Other, Destination; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216084 Copy
Species: Other
Genetic Insert: gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
Vector Backbone Description: Vector Backbone:pRDB_053; Vector Types:Insect Expression, Other, Destination; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216083 Copy
Species: Other
Genetic Insert: CD47 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Positive Controls; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216086 Copy
Species: Other
Genetic Insert: CD47 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Positive Controls; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216085 Copy
Species: Homo sapiens
Genetic Insert: FANCD2
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: NM_001018115,NM_001319984,ENST00000383807,ENST00000419585 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144382 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Addgene #155184; Backbone Size:5575; Vector Backbone:XLone-Puro Cas13d-eGFP U6 BbsI; Vector Types:Mammalian Expression, Other, Piggybac transposon; Bacterial Resistance:Ampicillin
References:
Comments: A plasmid that expresses Cas13d is needed. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns are the gRNA sequence and Ms are reversed sequences:
FWD:5'- AAACNNNNNNNNNNNNN
RVS: 5'-AAAAMMMMMMMMMMM
Proper citation: RRID:Addgene_159756 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Destination; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216077 Copy
Species: Other
Genetic Insert: CD81 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Positive Controls; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216091 Copy
Species: Synthetic
Genetic Insert: GFP and luciferase
Vector Backbone Description: Vector Backbone:PLOV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.17.431647v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_161030 Copy
Species: Other
Genetic Insert: CD97, CD4, CD26, CD274 guides
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Positive Controls; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216095 Copy
Species: Other
Genetic Insert: Cas9 [Sp]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Assembled Vector; Bacterial Resistance:Ampicillin
References:
Comments: lentiCRISPRv2 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216097 Copy
Species: Other
Genetic Insert: Cas12a [EnAs]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Assembled Vector; Bacterial Resistance:Ampicillin
References:
Comments: pRDA_550 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216096 Copy
Species: Other
Genetic Insert: CD59 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Positive Controls; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216087 Copy
Species: Other
Genetic Insert: CD47 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Other, Positive Controls; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216089 Copy
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