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Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: This encodes a secreted
antagonist of Xwnt-8.
The entire open reading frame of sizzled was amplified by low-cycle number PCR using Pfu polymerase and cloned as a
BamHI-XhoI fragment into pCS2+ .
Proper citation: RRID:Addgene_16688 Copy
Species: Xenopus laevis
Genetic Insert: APC
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: Full length Xenopus APC with an amino terminal c-myc epitope in the vector pCS2+MT.
Insert size: ~8.5kb. NcoI site introduced at 5' end. Linearize with Not1 and transcribe with SP6.
Proper citation: RRID:Addgene_16686 Copy
Species: Xenopus laevis
Genetic Insert: XCtBP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid History:
Constructed by Jeff Brown.
Notes for Use:
The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT.
Proper citation: RRID:Addgene_16704 Copy
Species: Xenopus laevis
Genetic Insert: Engrailed-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Other, Xenopus Probe; Bacterial Resistance:Ampicillin
References:
Comments: In situ probe cut with Xba1, transcribe with T3.
Proper citation: RRID:Addgene_16950 Copy
Species: Xenopus laevis
Genetic Insert: banded hedgehog
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Xenopus Hedgehog. Antisense are generated as listed:
Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.
Proper citation: RRID:Addgene_16984 Copy
Species: Xenopus laevis
Genetic Insert: Goosecoid
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian by PCR.
Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.
Proper citation: RRID:Addgene_16979 Copy
Species: Xenopus laevis
Genetic Insert: Xbra
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: full length Xbra from Jim Smith.
Antisense: linearize with EcoRV, transcribe with T7.
Plasmid History:
Constructed by Jan Christian.
Proper citation: RRID:Addgene_16978 Copy
Species: Xenopus laevis
Genetic Insert: noggin PCR clone
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: noggin PCR clone (coding region). In reverse orientation compared to CSKA promoter.
Plasmid History:
Constructed by Jan Christian.
Isolated and characterize by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16998 Copy
Species: Xenopus laevis
Genetic Insert: noggin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: noggin PCR clone (coding region) in SP64T.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian.
Noggin isolated and chacterized by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16997 Copy
Species: Xenopus laevis
Genetic Insert: dominant N-cadherin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length N-cadherin was digested with BamHI to remove nt 90-2004, deleting the extracellular domain while
keeping the signal sequence (identical to preparation by C. Kintner, 1992, Cell 69:225-236, who provided the cadherin).
Linearize and transcribe with T7.
Proper citation: RRID:Addgene_17025 Copy
Species: Xenopus laevis
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Description: This is an amino terminal HIS tag, then beta catenin domains R10C, subcloned into BamH1 site.
Reference: Made by Ken-Ichi Takemaru.
Contains ARM domains 8-10 and C-terminal end of protein (starts with aa519)
Proper citation: RRID:Addgene_17202 Copy
Species: Xenopus laevis
Genetic Insert: wbp2nlT45A
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: we cloned this plasmid
Proper citation: RRID:Addgene_172464 Copy
Species: Xenopus laevis
Genetic Insert: xFAM3B.L
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_172852 Copy
Species: Xenopus laevis
Genetic Insert: PABPC1 (M161A)
Vector Backbone Description: Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin
References:
Comments: The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length.
Proper citation: RRID:Addgene_174349 Copy
Species: Xenopus laevis
Genetic Insert: ePAB
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_174359 Copy
Species: Xenopus laevis
Genetic Insert: VSP1 R152Q
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Other, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51881 Copy
Species: Xenopus laevis
Genetic Insert: VSP1 C301S
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Other, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51882 Copy
Species: Xenopus laevis
Genetic Insert: His6-Thrombin-Xenopus laevis histones H2A
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_66890 Copy
Species: Xenopus laevis
Genetic Insert: Tyrosine-protein kinase transmembrane receptor ROR2
Vector Backbone Description: Backbone Size:4085; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Other, in vitro transcription; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67162 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus laevis beta-tubulin gene promoter region and 5'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4110; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_74102 Copy
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