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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCS2+ Sizzled Resource Report Resource Website |
RRID:Addgene_16688 | Sizzled | Xenopus laevis | Ampicillin | PMID:9428410 | This encodes a secreted antagonist of Xwnt-8. The entire open reading frame of sizzled was amplified by low-cycle number PCR using Pfu polymerase and cloned as a BamHI-XhoI fragment into pCS2+ . | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:31:47 | 0 | |
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Xenopus APC-myc Resource Report Resource Website |
RRID:Addgene_16686 | APC | Xenopus laevis | Ampicillin | PMID:9015311 | Insert: Full length Xenopus APC with an amino terminal c-myc epitope in the vector pCS2+MT. Insert size: ~8.5kb. NcoI site introduced at 5' end. Linearize with Not1 and transcribe with SP6. | Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:31:46 | 0 | |
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XE90 pCS2P+ XCtBP Resource Report Resource Website |
RRID:Addgene_16704 | XCtBP | Xenopus laevis | Ampicillin | PMID: | Plasmid History: Constructed by Jeff Brown. Notes for Use: The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT. | Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:31:48 | 0 | |
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pBluescript Engrailed-2 Resource Report Resource Website |
RRID:Addgene_16950 | Engrailed-2 | Xenopus laevis | Ampicillin | PMID:1679005 | In situ probe cut with Xba1, transcribe with T3. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Other, Xenopus Probe; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:16 | 0 | |
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XP6 Banded hh Resource Report Resource Website |
RRID:Addgene_16984 | banded hedgehog | Xenopus laevis | Ampicillin | PMID: | Xenopus Hedgehog. Antisense are generated as listed: Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert. Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert. Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:18 | 0 | |
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XP11 Goosecoid Resource Report Resource Website |
RRID:Addgene_16979 | Goosecoid | Xenopus laevis | Ampicillin | PMID: | Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation. Transcription: linearize with EcoR1 and transcribe with SP6. Plasmid History: Constructed by Jan Christian by PCR. Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA. | Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:18 | 0 | |
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XP12 Xbra Resource Report Resource Website |
RRID:Addgene_16978 | Xbra | Xenopus laevis | Ampicillin | PMID: | Insert: full length Xbra from Jim Smith. Antisense: linearize with EcoRV, transcribe with T7. Plasmid History: Constructed by Jan Christian. | Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:17 | 0 | |
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XE18 Noggin-CSKA Resource Report Resource Website |
RRID:Addgene_16998 | noggin PCR clone | Xenopus laevis | Ampicillin | PMID: | Insert: noggin PCR clone (coding region). In reverse orientation compared to CSKA promoter. Plasmid History: Constructed by Jan Christian. Isolated and characterize by W. Smith and R. Harland. | Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:21 | 0 | |
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XE17 Noggin Resource Report Resource Website |
RRID:Addgene_16997 | noggin | Xenopus laevis | Ampicillin | PMID: | Insert: noggin PCR clone (coding region) in SP64T. Transcription: linearize with EcoR1 and transcribe with SP6. Plasmid History: Constructed by Jan Christian. Noggin isolated and chacterized by W. Smith and R. Harland. | Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:21 | 0 | |
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XE57 Delta N-cadherin Resource Report Resource Website |
RRID:Addgene_17025 | dominant N-cadherin | Xenopus laevis | Ampicillin | PMID: | Full-length N-cadherin was digested with BamHI to remove nt 90-2004, deleting the extracellular domain while keeping the signal sequence (identical to preparation by C. Kintner, 1992, Cell 69:225-236, who provided the cadherin). Linearize and transcribe with T7. | Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | nt 90-2004 deleted | 2022-04-22 03:32:26 | 0 |
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M61 pPET28c-Xenopus beta catenin domains R10C Resource Report Resource Website |
RRID:Addgene_17202 | beta catenin | Xenopus laevis | Kanamycin | PMID: | Description: This is an amino terminal HIS tag, then beta catenin domains R10C, subcloned into BamH1 site. Reference: Made by Ken-Ichi Takemaru. Contains ARM domains 8-10 and C-terminal end of protein (starts with aa519) | Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | domains R10C | 2022-04-22 03:32:59 | 0 |
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pCS2+-wbp2nlT45A Resource Report Resource Website |
RRID:Addgene_172464 | wbp2nlT45A | Xenopus laevis | Ampicillin | PMID:28663133 | we cloned this plasmid | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | changed Threonine at aa45 to Alanine | 2022-04-22 03:33:02 | 0 |
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pCS2-xFAM3b.L-HA Resource Report Resource Website |
RRID:Addgene_172852 | xFAM3B.L | Xenopus laevis | Ampicillin | PMID:30209221 | Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:33:06 | 0 | ||
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C233 Resource Report Resource Website |
RRID:Addgene_174349 | PABPC1 (M161A) | Xenopus laevis | Ampicillin | PMID:34213414 | The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length. | Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin | M161A | 2022-04-22 03:33:19 | 0 |
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C205 Resource Report Resource Website |
RRID:Addgene_174359 | ePAB | Xenopus laevis | Ampicillin | PMID:34213414 | Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Multiple silent mutations introduced to be resistant to siRNAs | 2022-04-22 03:33:20 | 0 | |
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pcDNA3-Xl-VSP1 R152Q Resource Report Resource Website |
RRID:Addgene_51881 | VSP1 R152Q | Xenopus laevis | Ampicillin | PMID:21618529 | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Other, Xenopus oocyte expression; Bacterial Resistance:Ampicillin | R152Q: near the extracellular side of the fourth transmembrane domain, shifts Xl-VSP1 phosphatase activation to more negative membrane potentials | 2022-04-22 03:43:02 | 0 | |
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pcDNA3-Xl-VSP1 C301S Resource Report Resource Website |
RRID:Addgene_51882 | VSP1 C301S | Xenopus laevis | Ampicillin | PMID:21618529 | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Other, Xenopus oocyte expression; Bacterial Resistance:Ampicillin | C301S, catalytically-inactive mutant of Xl-VSP1 | 2022-04-22 03:43:02 | 0 | |
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pET29a-YS14 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66890 | His6-Thrombin-Xenopus laevis histones H2A | Xenopus laevis | Kanamycin | PMID:22617796 | Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:46:53 | 1 | ||
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pCS2 xRor2-mCherry Resource Report Resource Website |
RRID:Addgene_67162 | Tyrosine-protein kinase transmembrane receptor ROR2 | Xenopus laevis | Ampicillin | PMID:20732301 | Backbone Size:4085; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Other, in vitro transcription; Bacterial Resistance:Ampicillin | 2022-04-22 03:46:58 | 0 | ||
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pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2] Resource Report Resource Website |
RRID:Addgene_74102 | Xenopus laevis beta-tubulin gene promoter region and 5'UTR | Xenopus laevis | Ampicillin | PMID:25448690 | Backbone Marker:Invitrogen; Backbone Size:4110; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:48:54 | 0 |
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