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Species: Other
Genetic Insert: CD28-PafA
Vector Backbone Description: Vector Backbone:pEF6a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113400 Copy
Species: Other
Genetic Insert: type II-C anti-CRISPR
Vector Backbone Description: Backbone Size:4094; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113436 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid contains full-length IRS-1 protein. Cloned from human muscle cDNA library. (Kahn lab plasmid name = phIRS1-1)
Proper citation: RRID:Addgene_11359 Copy
Species: Homo sapiens
Genetic Insert: NM23 H1
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11364 Copy
Species: Homo sapiens
Genetic Insert: LARP7
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4972; Vector Backbone:pGEX-5X-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113545 Copy
Species: Homo sapiens
Genetic Insert: Rictor
Vector Backbone Description: Backbone Marker:BD Pharmingen; Backbone Size:4754; Vector Backbone:pRK-5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The amino acid sequence of this plasmid matches the NCBI published sequence. No functional differences between the plasmid with the point mutations (Addgene #1860) and this plasmid have been observed thus far. In this version, silent point mutations in the N-terminus were created to destroy the NotI site and facilitate cloning. These will not change the amino acid sequence of the protein. The ORF sequence of the new insert has been uploaded and is linked from this datasheet.
Proper citation: RRID:Addgene_11367 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:5938; Vector Backbone:pET His6 MBP N10 TEV LIC cloning vector (2C-T); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Cloning of pNS20-SpCas9-SNAP: pMJ922-SpyCas9-GFP bacterial expression vector was a kind gift from Prof. Martin Jinek. GFP was digested using BamHI and KpnI, and SNAPtag-NLS gBlocks (Integrated DNA Technologies) were integrated using In-Fusion HD Cloning Plus CE (Takara).
Proper citation: RRID:Addgene_113717 Copy
Species: Synthetic
Genetic Insert: jREX-GECO1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:4064; Vector Backbone:pTorPE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/04/435891 for bioRxiv preprint.
Proper citation: RRID:Addgene_113941 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4089; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The codon-optimized TIR1 is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117717 Copy
Species: Homo sapiens
Genetic Insert: Caspase 7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11815 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8 catalytic mutant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11818 Copy
Species: Homo sapiens
Genetic Insert: Caspase 8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11817 Copy
Species: Homo sapiens
Genetic Insert: Caspase 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11813 Copy
Species: Homo sapiens
Genetic Insert: Caspase 3
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11821 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results found S2A within the SUMO1 translation compared to the closest NCBI reference (NP_003343.1). The depositing laboratory affirms that to their knowledge, this mutation does not change protein function
Proper citation: RRID:Addgene_118361 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Other, Baculovirus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111745 Copy
Species: Homo sapiens
Genetic Insert: RNASEH1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:ppyCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Due to high GC content of the CAG promoter, there are some discrepancies in the promoter sequence. These are not known to affect the plasmid function.
Proper citation: RRID:Addgene_111906 Copy
Species: Homo sapiens
Genetic Insert: RNASEH1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:ppyCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Due to high GC content of the CAG promoter, there are some discrepancies in the promoter sequence. These are not known to affect the plasmid function.
Proper citation: RRID:Addgene_111905 Copy
Species: Synthetic
Genetic Insert: axon-GCaMP6s-P2A-mRuby3
Vector Backbone Description: Backbone Size:5109; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_112008 Copy
Species: Synthetic
Genetic Insert: NES-ZapCV2
Vector Backbone Description: Backbone Marker:Invitrogen (ThermoFisher / Life Technologies); Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_112060 Copy
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