Searching the RRID Resource Information Network
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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229957 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229961 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Other
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229960 Copy
Species: Other
Genetic Insert: tnaA-
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Other, This is a strain, not a plasmid; Bacterial Resistance:Other
References:
Comments: Derived from E. coli K-12 MG1655 strain. This strain does not produce indole as measured with Kovac's Reagent Indole Test. A T7 phage strain with a gp1 KO is available from the Whitehead Lab.
Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.
Proper citation: RRID:Addgene_228513 Copy
Species: Other
Genetic Insert: ΔdapA:RK2-ΔoriT:
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: The DH10B-MOB strain is a conjugation donor used to deliver plasmids containing an IncP (e.g., RK2/RP4) conjugation origin of transfer (oriT) to recipient cells. Conjugation has been used to easily deliver plasmids to a diverse range of bacteria, as well as to yeast, other fungi, and diatoms.
For more information on strain construction, usage, and conjugation protocols, please see the supplementary information in “XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera Xanthobacter and Roseixanthobacter.” (doi: 10.1021/acssynbio.4c00806)
The strain was made using a derivative of pTA-Mob (PMCID: PMC6829330; PMC3940858). A DH10B background was made that contains this RK2/RP4 conjugation machinery—but not the oriT sequence—integrated at the dapA locus. The dapA gene is disabled, therefore, to grow the strain one must supplement growth media with diaminopimelic acid (60 ug/mL). Likewise, to select against the conjugation donor after conjugation, do not add diaminopimelic acid to the media (i.e., final recipient strain selection plates).
Any plasmid containing the RK2/RP4 IncP oriT can be mobilized by this strain (AddGene #165983, AddGene #229357, AddGene #229388) and an oriT must be included on your plasmid of choice to deliver by conjugation.
Proper citation: RRID:Addgene_229395 Copy
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