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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
PPE18-HA
 
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RRID:Addgene_231246 PPE18-HA Other Hygromycin PMID: Backbone Size:6512; Vector Backbone:pBB115; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2025-03-05 01:09:05 0
pEB multi-Hyg Human collagen type IV alpha 4 chain (COL4A4)
 
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RRID:Addgene_229771 Human collagen type IV alpha 4 chain (COL4A4) Homo sapiens Hygromycin PMID:29526710 Backbone Marker:Fujifilm/Wako; Vector Backbone:pEBMulti-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2025-04-02 02:09:18 0
pKCyR8
 
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RRID:Addgene_149494 CymR(AM)-mRFP Synthetic Hygromycin PMID:34125913 Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin 2022-04-22 03:27:53 0
pBSV2H_Psyn-sgRNA500
 
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RRID:Addgene_149559 Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2022-04-22 03:27:54 0
pBSV2H_Psyn-sgRNAftsI
 
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RRID:Addgene_149563 sgRNAftsI Synthetic Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2022-04-22 03:27:54 0
pBbdCas9H(RBSmut)_arr2
 
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RRID:Addgene_149580 Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(RBSmut); Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:27:54 0
pBbdCas9H_arr2
 
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RRID:Addgene_149573 Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2022-04-22 03:27:54 0
pTE-28S15-0X
 
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RRID:Addgene_20319 Psmyc-tetR#28 synthetic gene derived from Tn10 tetR Hygromycin PMID:19174563 There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function. Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:35:39 0
pSE100
 
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RRID:Addgene_17972 pmyc1tetO Hygromycin PMID:17483222 pmyc1tetO is a tet operator containing promoter for mycobacteria. Also see Pubmed IDs 18059281 and 15687379. Derivative of pMS2 containing Pmyc1tetO. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators. Backbone Size:0; Vector Backbone:pMS2; Vector Types:Other, mycobacteria expression; Bacterial Resistance:Hygromycin 2022-04-22 03:34:33 0
pTE1062
 
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RRID:Addgene_172356 scbR Hygromycin PMID:29130169 Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425. pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase. Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Other, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin 2022-04-22 03:33:01 0
pSIMcpf1
 
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RRID:Addgene_153034 Ascpf1 Other Hygromycin PMID:33710766 Vector Backbone:pSIM18; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:28:16 0
pCR-Hyg
 
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RRID:Addgene_158708 crRNA Other Hygromycin PMID:28646112 Please note that several differences were identified in the plasmid backbone compared to the depositor's sequence. These differences do not affect plasmid function. Backbone Marker:from Houhui Song's Lab; Vector Backbone:pSL001; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Other, Mycobacteria Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:29:30 0
pYC2085
 
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RRID:Addgene_158721 Sth1 sgRNA scaffold Other Hygromycin PMID:31992616 insert sizes: 171 bp and 3366 bp Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2022-04-22 03:29:30 0
pSMT3-M
 
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RRID:Addgene_26589 n/a Hygromycin PMID:20352111 Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488. There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function. Backbone Size:5708; Vector Backbone:pSMT3; Vector Types:Other, Shuttle vector for mycobacteria; Bacterial Resistance:Hygromycin 2022-04-22 03:37:10 0
pGMEH-P38-che-ssrAec3
 
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RRID:Addgene_27059 P#38-mcherry rfp-ssrAec synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:37:16 0
pGMEH-P38-luxAB-ssrAec
 
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RRID:Addgene_27062 P#38-luxAB-ssrAec synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:37:16 0
pGMEH-P38-luxABct6
 
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RRID:Addgene_27063 P#38-luxAB-DAS+4 synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:37:16 0
pGMEH-10M1-clpX
 
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RRID:Addgene_27373 tetR(sB), clpXtb Hygromycin PMID:19174563 Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Other, Gateway Destination Vector; Bacterial Resistance:Hygromycin N/A 2022-04-22 03:37:21 0
pTEC15
 
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RRID:Addgene_30174 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Wasabi. The Wasabi ORF is present at bp# 39-749. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:00 0
pTEC17
 
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RRID:Addgene_30176 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Teal. The Teal ORF is present at bp# 103-813. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:00 0

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