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Species: Xenopus laevis
Genetic Insert: twisted gastrulation
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Best for in situs
For sense injection:
Cut with BamHI
Transcribe with SP6
Proper citation: RRID:Addgene_24680 Copy
Species: Xenopus laevis
Genetic Insert: secreted frizzled-related sequence protein 2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense linearize with NotI, transcribe with SP6.
Proper citation: RRID:Addgene_24696 Copy
Species: Xenopus laevis
Genetic Insert: Crescent
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense linearize with NotI, transcribe with SP6.
Proper citation: RRID:Addgene_24697 Copy
Species: Xenopus laevis
Genetic Insert: Frzb-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For antisense linearize with HindIII, transcribe with T7
For sense linearize with NotI, transcribe with SP6
Proper citation: RRID:Addgene_24694 Copy
Species: Xenopus laevis
Genetic Insert: DAN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For antisense linearize with EcoRI, transcribe with T7
For sense linearize with NotI, transcribe with SP6
Proper citation: RRID:Addgene_24693 Copy
Species: Xenopus laevis
Genetic Insert: insulin-like growth factor 1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: mRNA injection sense: cut with NotI, transcribe with Sp6
Anti-sense in situ probe: cut with EcoRI, transcribe with T7
PCR fragment of IGF1 (EcoRI-XhoI) from stage 27 cDNA inserted into pCS2 (EcoRI-XhoI).
Sense: 5'AAA GAA TTC ATG GAA ACA AAC AAC AAT
Antisense: 5'-TTT CTC GAG TTA CAT TCG GAA TCC TCG
Proper citation: RRID:Addgene_24967 Copy
Species: Xenopus laevis
Genetic Insert: Btg-X
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense mRNA cut with NotI, transcribe with SP6
For antisense cut with ClaI, transcribe with T7
Proper citation: RRID:Addgene_24964 Copy
Species: Xenopus laevis
Genetic Insert: Antivin
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For antisense cut with HindIII, transcribe with T7
For sense cut with NotI, transcribe with SP6
Proper citation: RRID:Addgene_24961 Copy
Species: Xenopus laevis
Genetic Insert: Frzb-1 HA
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense, cut with NotI, transcribe with SP6.
HA tag is cloned between Ser158 and Met159 of Frezzled by PCR. HA tag includes a AatII diagnostic site.
Proper citation: RRID:Addgene_24979 Copy
Species: Xenopus laevis
Genetic Insert: Chordin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS-SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Partial 2.8 kB clone of the original Xenopus chordin cDNA lacking 3' portion.
For antisense cut with EcoRI, transcribe with T7
For Northern blot probe, cut with EcoRI and XhoI
Proper citation: RRID:Addgene_24975 Copy
Species: Xenopus laevis
Genetic Insert: lhx1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: HNF1 binding site in lhx1 promoter: TTAATGGTTTAC
Proper citation: RRID:Addgene_30492 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus FGFR4-ICD-FRB
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31262 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus FGFR4-ICD-FKBP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31260 Copy
Species: Xenopus laevis
Genetic Insert: cardiac actin / Cre
Vector Backbone Description: Backbone Size:3000; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pCARGFP (Kroll et al 1996) used to replace the GFP with Cre from pCSCre2
Proper citation: RRID:Addgene_31309 Copy
Species: Xenopus laevis
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32793 Copy
Species: Xenopus laevis
Genetic Insert: Ecd8
Vector Backbone Description: Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:Other, Xenopus RNA expression ; Bacterial Resistance:Ampicillin
References:
Comments: Digest with Pst1, transcribe with T7 pol for sense RNA.
Proper citation: RRID:Addgene_32992 Copy
Species: Xenopus laevis
Genetic Insert: Xfz8
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32990 Copy
Species: Xenopus laevis
Genetic Insert: HIPK2
Vector Backbone Description: Backbone Marker:Sergei Sokol; Vector Backbone:pCTX-myc; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32996 Copy
Species: Xenopus laevis
Genetic Insert: XTCF3
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See author's map for complete restriction site details.
Proper citation: RRID:Addgene_32998 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt2b
Vector Backbone Description: Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:Other, RNA Synthesis and Injections; Bacterial Resistance:Ampicillin
References:
Comments: Addgene quality control sequencing discovered a P159A point mutation. According to the depositing lab, this mutation is not a concern, as the product is functionally active.
Proper citation: RRID:Addgene_32988 Copy
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