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  • Organism:xenopus laevis (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pcDNA3 xenopus twisted gastrulation
 
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RRID:Addgene_24680 twisted gastrulation Xenopus laevis Ampicillin PMID:10866189 Best for in situs For sense injection: Cut with BamHI Transcribe with SP6 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:45 0
pCS2 xenopus sFRP2
 
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RRID:Addgene_24696 secreted frizzled-related sequence protein 2 Xenopus laevis Ampicillin PMID:10960783 For sense linearize with NotI, transcribe with SP6. Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:46 0
pCS2 xCrescent-HA
 
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RRID:Addgene_24697 Crescent Xenopus laevis Ampicillin PMID:10960783 For sense linearize with NotI, transcribe with SP6. Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:46 0
pCS2 xenopus Frzb-1
 
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RRID:Addgene_24694 Frzb-1 Xenopus laevis Ampicillin PMID:10960783 For antisense linearize with HindIII, transcribe with T7 For sense linearize with NotI, transcribe with SP6 Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:45 0
pCS2 xenopus DAN
 
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RRID:Addgene_24693 DAN Xenopus laevis Ampicillin PMID:16172975 For antisense linearize with EcoRI, transcribe with T7 For sense linearize with NotI, transcribe with SP6 Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:45 0
pCS2 xIGF1
 
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RRID:Addgene_24967 insulin-like growth factor 1 Xenopus laevis Ampicillin PMID:11709186 mRNA injection sense: cut with NotI, transcribe with Sp6 Anti-sense in situ probe: cut with EcoRI, transcribe with T7 PCR fragment of IGF1 (EcoRI-XhoI) from stage 27 cDNA inserted into pCS2 (EcoRI-XhoI). Sense: 5'AAA GAA TTC ATG GAA ACA AAC AAC AAT Antisense: 5'-TTT CTC GAG TTA CAT TCG GAA TCC TCG Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:50 0
pCS2 xBtg-X
 
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RRID:Addgene_24964 Btg-X Xenopus laevis Ampicillin PMID:15879552 For sense mRNA cut with NotI, transcribe with SP6 For antisense cut with ClaI, transcribe with T7 Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:50 0
pCS2 Antivin
 
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RRID:Addgene_24961 Antivin Xenopus laevis Ampicillin PMID:16172975 For antisense cut with HindIII, transcribe with T7 For sense cut with NotI, transcribe with SP6 Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:50 0
pCS2 xFrzb-HA
 
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RRID:Addgene_24979 Frzb-1 HA Xenopus laevis Ampicillin PMID:9118218 For sense, cut with NotI, transcribe with SP6. HA tag is cloned between Ser158 and Met159 of Frezzled by PCR. HA tag includes a AatII diagnostic site. Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:51 0
pBS xChordin
 
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RRID:Addgene_24975 Chordin Xenopus laevis Ampicillin PMID:8001117 Partial 2.8 kB clone of the original Xenopus chordin cDNA lacking 3' portion. For antisense cut with EcoRI, transcribe with T7 For Northern blot probe, cut with EcoRI and XhoI Backbone Size:3000; Vector Backbone:pBS-SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:51 0
Ex1(-120/+3)
 
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RRID:Addgene_30492 lhx1 promoter Xenopus laevis Ampicillin PMID:21281489 HNF1 binding site in lhx1 promoter: TTAATGGTTTAC Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2022-04-22 03:38:05 0
iXFGFR4r
 
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RRID:Addgene_31262 Xenopus FGFR4-ICD-FRB Xenopus laevis Ampicillin PMID:20013652 Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:14 0
iXFGFR4k
 
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RRID:Addgene_31260 Xenopus FGFR4-ICD-FKBP Xenopus laevis Ampicillin PMID:20013652 Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:14 0
pCARCre
 
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RRID:Addgene_31309 cardiac actin / Cre Xenopus laevis Ampicillin PMID:12682379 pCARGFP (Kroll et al 1996) used to replace the GFP with Cre from pCSCre2 Backbone Size:3000; Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:14 0
pcDNA3.1-xASIC1
 
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RRID:Addgene_32793 ASIC1 Xenopus laevis Ampicillin PMID: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:29 0
ECD8-pXT7
 
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RRID:Addgene_32992 Ecd8 Xenopus laevis Ampicillin PMID:10485853 Digest with Pst1, transcribe with T7 pol for sense RNA. Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:Other, Xenopus RNA expression ; Bacterial Resistance:Ampicillin Contains the first 230 aa of Xfz8 2022-04-22 03:38:31 0
Xfz8-pCS2
 
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RRID:Addgene_32990 Xfz8 Xenopus laevis Ampicillin PMID:9651509 Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:31 0
pCTX-myc-HIPK2 WT
 
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1+ mentions
RRID:Addgene_32996 HIPK2 Xenopus laevis Ampicillin PMID:20951344 Backbone Marker:Sergei Sokol; Vector Backbone:pCTX-myc; Vector Types:; Bacterial Resistance:Ampicillin 2022-04-22 03:38:31 1
pCS2+Flag-XTCF3-HA
 
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RRID:Addgene_32998 XTCF3 Xenopus laevis Ampicillin PMID:20951344 See author's map for complete restriction site details. Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:31 0
Xwnt2b-pXT7
 
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RRID:Addgene_32988 Xwnt2b Xenopus laevis Ampicillin PMID:9203142 Addgene quality control sequencing discovered a P159A point mutation. According to the depositing lab, this mutation is not a concern, as the product is functionally active. Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:Other, RNA Synthesis and Injections; Bacterial Resistance:Ampicillin 2022-04-22 03:38:31 0

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