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Plasmids are provided by Addgene and DGRC.

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  • Organism:gallus gallus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBS chick islet1 [TJ#44]
 
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RRID:Addgene_16273 islet1 Gallus gallus Ampicillin PMID: To make a sense probe, cut with XhoI and transcribe with T3. To make an antisense probe, cut with NotI transcribe with T7. Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:30:41 0
pBS chick LH-2A [TJ#93]
 
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RRID:Addgene_16280 LH-2A Gallus gallus Ampicillin PMID: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:30:42 0
pCAGGS-Hoxa7
 
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RRID:Addgene_45554 Hoxa7 Gallus gallus Ampicillin PMID:23359544 Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:Other; Bacterial Resistance:Ampicillin 2022-04-22 03:41:25 0
pEXP5-NT-AlleyCat
 
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RRID:Addgene_41715 mutated C-terminal portion of calmodulin gene (M76-K148) Gallus gallus Ampicillin PMID:21482808 Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin F92E 2022-04-22 03:40:29 0
pEXP5-NT-7th R
 
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RRID:Addgene_42573 mutated calmodulin gene Gallus gallus Ampicillin PMID:23630096 Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed Phenylalanine 92 to Glutamine acid, Isoleucine 85 to Leucine, Alanine 88 to Glutamine, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Methionine 124 to Leucine, Alanine 128 to Threonine, Methionine 144 to Arginine 2022-04-22 03:40:46 0
pAc-Neo-OVA
 
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RRID:Addgene_22533 OVA Gallus gallus Ampicillin PMID:3261634 The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector. Backbone Marker:Kedes Lab; Backbone Size:10200; Vector Backbone:pHBetaAPr-1-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:03 0
cFGF8
 
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RRID:Addgene_22089 FGF8 Gallus gallus Ampicillin PMID:8548816 Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region. For antisense probe linearize with EcoRI and use T7 Polymerase. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK (-); Vector Types:Other, Bacterial; Bacterial Resistance:Ampicillin 2022-04-22 03:35:56 0
4CE1A cSpry1
 
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RRID:Addgene_22095 Sprouty 1 Gallus gallus Ampicillin PMID: The partial clone contains ~230bp coding region and 3' UTR sequences. For antisense probe, linearize the plasmid with XbaI and use T3 Polymerase. For negative control sense probe linearize with XhoI and transcribe with T7 Polymerase. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Other, Bacterial; Bacterial Resistance:Ampicillin 2022-04-22 03:35:57 0
3CB1/cSprouty2
 
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RRID:Addgene_22096 Sprouty 2 Gallus gallus Ampicillin PMID:10498682 Partial chicken Sprouty2 cDNA clone was isolated from a chick embryonic brain cDNA library (Lambda Zap). The entire coding region and 3' UTR sequences are included. For antisense probe, linearize the plasmid with EcoRI and use T7 Polymerase. The transcript should be approximately 1.4kb. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Other, Bacterial; Bacterial Resistance:Ampicillin 2022-04-22 03:35:57 0
pcTUBB3
 
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RRID:Addgene_31232 TUBB3 Gallus gallus Ampicillin PMID:12486167 For riboprobes, cut with EagI. Runoffs with T7. Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Other, cDNA for in situ hybridization; Bacterial Resistance:Ampicillin C-terminal + 3'UTR sequence 2022-04-22 03:38:14 0
pXeX-SHH
 
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RRID:Addgene_31233 SHH Gallus gallus Ampicillin PMID:11251119 Backbone Marker:A. D. Johnson, P. A. Krieg, Dev. Genet. 17, 280 (1995); Vector Backbone:pXeX; Vector Types:Other, Xenopus expression vector; Bacterial Resistance:Ampicillin starts at aa4 2022-04-22 03:38:14 0
pcDISP1
 
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RRID:Addgene_31235 DISP1 Gallus gallus Ampicillin and Kanamycin PMID:16246323 For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF. Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:Other, cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin 2022-04-22 03:38:14 0
pOVA
 
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RRID:Addgene_31598 OVA Gallus gallus Ampicillin PMID:9364701 *The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details. Backbone Marker:Reff et al., 1994; Backbone Size:9209; Vector Backbone:TCAE (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:16 0
AP-SEMA3C (chick)
 
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RRID:Addgene_29449 AP-SEMA3C Gallus gallus Ampicillin PMID:9331347 Backbone Marker:modified from PCDNA3 see methods in associated publication.; Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:37:49 0
AP-SEMA3A (chick)
 
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1+ mentions
RRID:Addgene_29448 AP-SEMA3A (chick) Gallus gallus Ampicillin PMID:9331347 Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:37:49 1
SEMA3A (chick) pBLUE
 
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RRID:Addgene_29564 SEMA3A (chick) Gallus gallus Ampicillin PMID: To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase. Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A. Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:Other, pBlue for ISH; Bacterial Resistance:Ampicillin 2.1kb fragment for in situ hybridization (ISH) 2022-04-22 03:37:51 0
1XDR4
 
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RRID:Addgene_32614 1XDR4 Gallus gallus Ampicillin PMID:21060789 Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function. Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:27 0
aTRE
 
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RRID:Addgene_32612 Thyroid Response Element Gallus gallus Ampicillin PMID:21060789 Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:27 0
cLS-F2
 
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RRID:Addgene_32617 rGH-PAL Gallus gallus Ampicillin PMID:21060789 Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:38:27 0
pMvsrcD
 
Resource Report
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RRID:Addgene_17670 v-src Gallus gallus Ampicillin PMID:2161957 Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:33:59 0

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