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Species: Synthetic
Genetic Insert: sgRNA_6E
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: MoClo compatible
Proper citation: RRID:Addgene_176870 Copy
Species: Other
Genetic Insert: CmYLCV
Vector Backbone Description: Vector Backbone:plCH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: MoClo compatible
Proper citation: RRID:Addgene_176872 Copy
Species: Synthetic
Genetic Insert: sgRNA_4
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: MoClo compatible
Proper citation: RRID:Addgene_176867 Copy
Species: Synthetic
Genetic Insert: sgRNA_4E
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: MoClo compatible
Proper citation: RRID:Addgene_176868 Copy
Species: Other
Genetic Insert: 35S terminator
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: MoClo compatible
Proper citation: RRID:Addgene_176881 Copy
Species: Arabidopsis thaliana
Genetic Insert: HSP terminator
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: MoClo compatible
Proper citation: RRID:Addgene_176882 Copy
Species: Homo sapiens
Genetic Insert: RALA
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_70500 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_173926 Copy
Species:
Genetic Insert: NLS-Cas12j2-NLS
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_173924 Copy
Species:
Genetic Insert: UNG-rAPOBEC1(R33A)-zCas9(D10A)
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_173997 Copy
Species:
Genetic Insert: rAPOBEC1-zCas9(D10A)-UNG
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_173998 Copy
Species:
Genetic Insert: rAPOBEC1-zCas9(D10A)-rXRCC1
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_173999 Copy
Species:
Genetic Insert: UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_174000 Copy
Species: Other
Genetic Insert: PhoQ; lacI
Vector Backbone Description: Backbone Size:1885; Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Please note: Plasmid contains a S286L mutation in lacI. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_181952 Copy
Species: Other
Genetic Insert: NarX; lacI
Vector Backbone Description: Backbone Size:1885; Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_181951 Copy
Species: Synthetic
Genetic Insert: P35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160645 Copy
Species: Homo sapiens
Genetic Insert: SPRED1
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments: g290a, t1044c silent mutations not noted in Refseq
Proper citation: RRID:Addgene_70605 Copy
Species: Homo sapiens
Genetic Insert: SCYL2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23458 Copy
Species: Homo sapiens
Genetic Insert: RAC2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments: c81g silent mutation not noted in Refseq
Proper citation: RRID:Addgene_70493 Copy
Species: Homo sapiens
Genetic Insert: PALI1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_114442 Copy
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