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Species: Mus musculus
Genetic Insert: C1V1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124650 Copy
Species: Homo sapiens
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124652 Copy
Species: Homo sapiens
Genetic Insert: P53 NLuc reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124526; Backbone Size:2328; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124533 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:5000; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology, Other; Bacterial Resistance:Spectinomycin
References:
Comments: Destination vector. BsmBI cloning site. Pink/White selection
Proper citation: RRID:Addgene_124528 Copy
Species: Homo sapiens
Genetic Insert: RGECO-TnT
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7500; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124643 Copy
Species: Other
Genetic Insert: Flavodoxin II
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5403; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124520 Copy
Species: Other
Genetic Insert: NL-DHFR-SpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:4000; Vector Backbone:pX330; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124522 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:2334; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: This is a new GB2.0-type vector, that expands the Alpha level. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124525 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:2330; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: This is a new GB2.0-type vector, that expands the Alpha level. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124527 Copy
Species: Homo sapiens
Genetic Insert: INPPL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3 HIS C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124645 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:2336; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: This is a new GB2.0-type vector, that expands the Alpha level. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124526 Copy
Species:
Genetic Insert: TurboID
Vector Backbone Description: Vector Backbone:pCD-betaG-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: A TurboID cDNA was amplified by PCR from V5-TurboID-NES_pcDNA3 (Plamid #107169).
Proper citation: RRID:Addgene_124646 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3599; Vector Backbone:pM9-4Compln; Vector Types:Other, Vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin
References:
Comments: This is a modify vector compared to pPTbsr
Proper citation: RRID:Addgene_124519 Copy
Species:
Genetic Insert: CMVe-betaAc-STOP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMVe-betaAc-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: CMV-enhancer-betaAc driven luciferase reporter gene containing a floxed stop cassette.
Proper citation: RRID:Addgene_12463 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Marker:https://www.addgene.org/19407/; Backbone Size:4200; Vector Backbone:pTretight-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124506 Copy
Species: Synthetic
Genetic Insert: cggR_R250A
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124585 Copy
Species: Synthetic
Genetic Insert: cggR
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124584 Copy
Species: Homo sapiens
Genetic Insert: ADAR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
References:
Comments: Also expresses 2 copies of GluR2_adRNA(1,20,6) from human and mouse U6 promoters
Proper citation: RRID:Addgene_124620 Copy
Species: Homo sapiens
Genetic Insert: MCP_ADAR1 DD (E1008Q)_NLS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
References:
Comments: Also expresses MS2_adRNA targeting the RAB7A transcript
Proper citation: RRID:Addgene_124626 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector).
To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted.
The mTurquoise2 tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124504 Copy
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