Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
GB1-His-TET1-878-911-L900A(pL91) Resource Report Resource Website |
RRID:Addgene_124395 | GB1-His-TET1-878-911-L900A | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 91; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | L900A | 2022-04-22 03:21:57 | 0 | |
|
FLAG-FullLength-TET1-LLAA(20) Resource Report Resource Website |
RRID:Addgene_124397 | FLAG-FullLength-TET1 with L897A/L900A mutations | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 38; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | L897A/L900A mutations (full length protein is 2136 aa) | 2022-04-22 03:21:58 | 0 | |
|
GB1-His-TET1-878-911(pL91) Resource Report Resource Website |
RRID:Addgene_124390 | GB1-His-TET1-878-911 wildtype | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 91; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:57 | 0 | ||
|
GB1-His-SAP25-126-186(pL91) Resource Report Resource Website |
RRID:Addgene_124387 | GB1-His-SAP25-126-186 | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 91; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:57 | 0 | ||
|
pCDNA3-HA PSPC1 Y523F Resource Report Resource Website |
RRID:Addgene_124373 | PSPC1 | Homo sapiens | Ampicillin | PMID:31844057 | For wildtype PSPC1 see https://www.addgene.org/101764/ | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Y523F | 2022-04-22 03:21:57 | 0 |
|
pAV-U6+27-Tornado-BroccoliSamSensor#1 Resource Report Resource Website |
RRID:Addgene_124363 | Tornado-BroccoliSamSensor#1 | Ampicillin | PMID:30962542 | Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:57 | 0 | |||
|
Gal4-TET1-878-911-WT(pL84) Resource Report Resource Website |
RRID:Addgene_124400 | Gal4-TET1-878-911 wildtype | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:58 | 0 | ||
|
pLG173 Resource Report Resource Website |
RRID:Addgene_124368 | empty backbone (control) | Synthetic | Ampicillin | PMID:31792444 | Vector Backbone:pLG166; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:21:57 | 0 | ||
|
Gal4-TET1-878-911-I894A(pL84) Resource Report Resource Website |
RRID:Addgene_124404 | Gal4-TET1-878-911(I894A mutation) | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | I894A | 2022-04-22 03:21:58 | 0 | |
|
Gal4-TET1-878-911-T898A(pL84) Resource Report Resource Website |
RRID:Addgene_124403 | Gal4-TET1-878-911(T898A mutation) | Homo sapiens | Ampicillin | PMID:30279502 | Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | T898A | 2022-04-22 03:21:58 | 0 | |
|
SpyDock Resource Report Resource Website |
RRID:Addgene_124618 | SpyDock | Synthetic | Ampicillin | PMID:30988307 | Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | E77A mutation prevents isopeptide bond formation, S49C mutation enables attachment to solid support. Mutations A89P, Q97D, K108E helps better SpyTag binding. | 2022-04-22 03:22:02 | 0 | |
|
pcDNA-PU.1-ETS N236H Resource Report Resource Website |
RRID:Addgene_124616 | PU.1 ETS domain (residues 167-272) | Homo sapiens | Ampicillin | PMID:30295801 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Residues 236 altered from asparagine to a histidine | 2022-04-22 03:22:02 | 0 | |
|
M50 Super 8x TOPFlash Resource Report Resource Website 10+ mentions |
RRID:Addgene_12456 | TCF/LEF binding sites | Ampicillin | PMID:12699626 | This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site). Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites. | Backbone Marker:Clontech; Backbone Size:4871; Vector Backbone:pTA-Luc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:01 | 28 | ||
|
AICSDP-69: NPM1-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_124608 | NPM1 Homology Arms with linker-mTagRFP-T | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the C-terminus of human NPM1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: KPNSAVDGTAGPGSIAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:02 | 0 | |
|
pGEX-mUbp43-His Resource Report Resource Website |
RRID:Addgene_12458 | UBP43 | Mus musculus | Ampicillin | PMID:11788588 | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:01 | 0 | ||
|
pFlagCMV2-14-3-3sigma Resource Report Resource Website |
RRID:Addgene_12453 | 14-3-3 sigma | Homo sapiens | Ampicillin | PMID:16352599 | Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:01 | 0 | ||
|
pcDNA3.0-HA-EFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_12452 | EFP | Homo sapiens | Ampicillin | PMID:16352599 | Digestion by KpnI and XhoI should give a 5.4 kb fragment and a 2.4 kb fragment. Compared to GenBank ID NP_005073.2, the EFP insert in this plasmid appears to contain a P385L mutation, whereas when compared to GenBank ID AB384815.1 the EFP insert appears to contain G89V and A185T mutations. These apparent mutations may represent valid polymorphisms and reflect the sequence of the original human cDNA clone. The plasmid is exactly as described in the associated publication. | Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:00 | 2 | |
|
pcDNA3.1-HA-mUbp43 Resource Report Resource Website |
RRID:Addgene_12454 | UBP43 | Mus musculus | Ampicillin | PMID:16710296 | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:01 | 0 | ||
|
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 Resource Report Resource Website 1+ mentions |
RRID:Addgene_124603 | Soma-localized ChrimsonR | Mus musculus | Ampicillin | PMID:30842660 | Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:02 | 1 | ||
|
3XFlag-pA-Tn5-Fl Resource Report Resource Website 1+ mentions |
RRID:Addgene_124601 | Protein A and hyperactive Tn5 transposase (Tnp) fusion protein | Other | Ampicillin | PMID:31036827 | Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences. This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:22:02 | 1 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
