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Species: Mus musculus
Genetic Insert: Sox2 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124197 Copy
Species: Homo sapiens
Genetic Insert: NGN2, ISL1, LHX3
Vector Backbone Description: Vector Backbone:CLYBL targeting; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124230 Copy
Species: Mus musculus
Genetic Insert: Klf2 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124199 Copy
Species: Other
Genetic Insert: CheRiff-tdTomato
Vector Backbone Description: Backbone Marker:from Harold Burgess lab (Addgene Plasmid #62215); Backbone Size:4548; Vector Backbone:pT1UciMP; Vector Types:Other, Zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato. In addition to the opsin gene, it contains tol1 arms, 14xUAS-E1b promoter, ubiquitin C intron, and ocean pout antifreeze protein terminator. Co-inject with Tol1 (not Tol2) transposase mRNA.
Proper citation: RRID:Addgene_124234 Copy
Species: Mus musculus
Genetic Insert: Nanog enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124190 Copy
Species: Mus musculus
Genetic Insert: Pou5f1 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124191 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pGL4.10; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: MCS: KpnI-XhoI, BamHI-SalI
Proper citation: RRID:Addgene_124185 Copy
Species: Other
Genetic Insert: MerCreMer-IRES-Zeo
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124184 Copy
Species:
Genetic Insert: OmpA-mNG-sfTq2ox
Vector Backbone Description: Vector Backbone:pTHV037; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124220 Copy
Species: Mus musculus
Genetic Insert: Klf4 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124186 Copy
Species: Mus musculus
Genetic Insert: Nanog enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124189 Copy
Species: Other
Genetic Insert: self-targeting gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Please note this plasmid contains Illumina 2nd step primer capture sites within the insert sequence that can affect next generation sequencing results. We suggest using sanger sequencing when working with this plasmid.
Proper citation: RRID:Addgene_124221 Copy
Species: Other
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_124226 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124332 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124331 Copy
Species: Homo sapiens
Genetic Insert: NFATc4 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124290 Copy
Species: Homo sapiens
Genetic Insert: Collagen type VII alpha 1 chain
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124286 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP2
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124321 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124320 Copy
Species: Homo sapiens
Genetic Insert: NFATc4 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124289 Copy
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