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Species: Xenopus laevis
Genetic Insert: frizzled-3
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: Removed 5'UTR from xfz3.
For sense transcripts, cut with NotI and transcribe with SP6.
Proper citation: RRID:Addgene_16327 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-7
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6
Proper citation: RRID:Addgene_16324 Copy
Species: Xenopus laevis
Genetic Insert: glycogen synthase kinase-3beta
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: His tagged Xenopus GSK-3.
For sense transcripts, cut with NotI and transcribe with SP6
Proper citation: RRID:Addgene_16335 Copy
Species: Xenopus laevis
Genetic Insert: Otx1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Isolated from Harland Stage 30 head library. Expressed in dorsal mesoderm and forebrain like Otx2, but also expressed in germ plasm.
Proper citation: RRID:Addgene_16332 Copy
Species: Xenopus laevis
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense mRNA, linearize with NotI and transcribe with SP6.
For antisense probe, cut with Pst1, remove 3' overhang, and transcribe with T7.
NcoI digest cuts out a 2333 bp fragment.
Proper citation: RRID:Addgene_16298 Copy
Species: Xenopus laevis
Genetic Insert: pt beta-catenin-myc
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pcS2+MT; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: This Xenopus Beta-catenin has four point mutations introduced into the GSK-3 phosphorylation site as shown.
The construct is derived from construct XE-28 and is linearized, transcribed, and used as described there, and in the original citation.
Ref: Yost et al., Genes & Development, 10:1443-1454, 1996.
four mutations are amino acids S33A, S37A, T41A, S45A
Proper citation: RRID:Addgene_16840 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-8
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:CS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: EcoR1 insert from pGEM Xwnt-8 subcloned into EcoR1 of CS2+;
Sense RNA cut with ASP718 and transcribe with SP6.
Constructed by Jeff Brown, unpublished.
Proper citation: RRID:Addgene_16865 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-4 myc
Vector Backbone Description: Backbone Size:2999; Vector Backbone:SP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: See clone XE2 for details. A single c-myc epitope recognized by the monoclonal antibody 9E10 has been addes to the C-terminus.
Transcription: linearize with Sal1 and transcribe with SP6.
Citation: L. Lynn McGrew, unpublished.
Proper citation: RRID:Addgene_16877 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-4myc
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: See clone XE2 for details and XE62 for myc tag, and V4 for vector details.
Notes for Use:
Citation: L. Lynn McGrew, unpublished.
Proper citation: RRID:Addgene_16876 Copy
Species: Xenopus laevis
Genetic Insert: full coding of Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: full coding of Xwnt-5A.
Insert size: 1.2 Kb.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by RTM/JR on the date of 4/93.
Notes for Use:
This construct encodes full coding sequence of Xwnt-5A, without wnt UTR's.
See Xwnt 5A full-length for Genbank data.
Proper citation: RRID:Addgene_16874 Copy
Species: Xenopus laevis
Genetic Insert: Wnt-5A
Vector Backbone Description: Backbone Marker:Available from Addgene (#17120); Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: The myc tagged Xwnt-5A insert of clone XE40, in the SV40 construct pCD-PS modified by Julia Yang-Snyder to include Xenopus beta globin flanking untranslated regions.
Reference: The clone was made by Julia Yang-Snyder.
Addgene QC found a G125A point mutation that is not known to affect function.
Proper citation: RRID:Addgene_16873 Copy
Species: Xenopus laevis
Genetic Insert: dnWnt-11
Vector Backbone Description: Backbone Marker:Available at Addgene (#15030); Backbone Size:3300; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler
et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB.
For injection, linearize with BamH1 and transcribe with SP6.
Proper citation: RRID:Addgene_16857 Copy
Species: Xenopus laevis
Genetic Insert: Ectodermin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCSP1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For Xenopus embryo mRNA microinjections, cut AscI and transcribe SP6.
Proper citation: RRID:Addgene_20900 Copy
Species: Xenopus laevis
Genetic Insert: 7XTCF binding sites, Siamois minimal promoter, NLS-lacz
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments: Wnt/beta-catenin responsive promoter in mouse tissues (used to derive the BAT-gal mouse strain), Xenopus embryos and human cell lines
Proper citation: RRID:Addgene_20889 Copy
Species: Xenopus laevis
Genetic Insert: 7xTCF binding sites, Siamois minimal promoter
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Wnt/beta-catenin responsive promoter in Xenopus embryos and human cell lines
Proper citation: RRID:Addgene_20890 Copy
Species: Xenopus laevis
Genetic Insert: -290 Mix.2 M-p53BE promoter luciferase
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGL3basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20894 Copy
Species: Xenopus laevis
Genetic Insert: Insulin 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Antisense: cut with EcoRI, transcribe with T7
Sense: cut with NotI, transcribe with SP6
Proper citation: RRID:Addgene_25786 Copy
Species: Xenopus laevis
Genetic Insert: Sox 17b
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For antisense RNA cut with ApaI, transcribe with SP6
Proper citation: RRID:Addgene_25782 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Best in situ probe.
For anitsense: linearize with NotI
Transcribe with SP6
For mRNA injections use 200 pg to dorsalize.
E248D does not affect function.
Proper citation: RRID:Addgene_24684 Copy
Species: Xenopus laevis
Genetic Insert: cerberus
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For injection cut with NotI.
Transcribe with SP6.
The construct fuses aa 1-25 (KHSEG25) to aa 117-270 (117RRKA)
Proper citation: RRID:Addgene_24681 Copy
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