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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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xfz3/pCS2+ Resource Report Resource Website |
RRID:Addgene_16327 | frizzled-3 | Xenopus laevis | Ampicillin | PMID:11585792 | Removed 5'UTR from xfz3. For sense transcripts, cut with NotI and transcribe with SP6. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:50 | 0 | |
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xfz7/pCS2+ Resource Report Resource Website |
RRID:Addgene_16324 | frizzled-7 | Xenopus laevis | Ampicillin | PMID:9636083 | For sense transcripts, cut with NotI and transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:50 | 0 | |
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GSK-3beta-his (Xenopus)/pCS2+ Resource Report Resource Website |
RRID:Addgene_16335 | glycogen synthase kinase-3beta | Xenopus laevis | Ampicillin | PMID: | His tagged Xenopus GSK-3. For sense transcripts, cut with NotI and transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | Xenopus GSK-3 with 6X his tag | 2022-04-22 03:30:52 | 0 |
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Otx1/pBS Resource Report Resource Website |
RRID:Addgene_16332 | Otx1 | Xenopus laevis | Ampicillin | PMID: | Isolated from Harland Stage 30 head library. Expressed in dorsal mesoderm and forebrain like Otx2, but also expressed in germ plasm. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:51 | 0 | |
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Axin/pCS2MT Resource Report Resource Website |
RRID:Addgene_16298 | Axin | Xenopus laevis | Ampicillin | PMID:10072781 | For sense mRNA, linearize with NotI and transcribe with SP6. For antisense probe, cut with Pst1, remove 3' overhang, and transcribe with T7. NcoI digest cuts out a 2333 bp fragment. | Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:45 | 0 | |
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XE49 pt Beta-catenin-myc Resource Report Resource Website |
RRID:Addgene_16840 | pt beta-catenin-myc | Xenopus laevis | Ampicillin | PMID:8666229 | This Xenopus Beta-catenin has four point mutations introduced into the GSK-3 phosphorylation site as shown. The construct is derived from construct XE-28 and is linearized, transcribed, and used as described there, and in the original citation. Ref: Yost et al., Genes & Development, 10:1443-1454, 1996. four mutations are amino acids S33A, S37A, T41A, S45A | Backbone Size:4352; Vector Backbone:pcS2+MT; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | four point mutations introduced into the GSK-3 phosphorylation site | 2022-04-22 03:32:05 | 0 |
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XE10 Xwnt-8 CS2+ Resource Report Resource Website |
RRID:Addgene_16865 | Xwnt-8 | Xenopus laevis | Ampicillin | PMID: | EcoR1 insert from pGEM Xwnt-8 subcloned into EcoR1 of CS2+; Sense RNA cut with ASP718 and transcribe with SP6. Constructed by Jeff Brown, unpublished. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:CS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:07 | 0 | |
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XE62 Xwnt-4 myc-SP64T Resource Report Resource Website |
RRID:Addgene_16877 | Xwnt-4 myc | Xenopus laevis | Ampicillin | PMID: | See clone XE2 for details. A single c-myc epitope recognized by the monoclonal antibody 9E10 has been addes to the C-terminus. Transcription: linearize with Sal1 and transcribe with SP6. Citation: L. Lynn McGrew, unpublished. | Backbone Size:2999; Vector Backbone:SP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:07 | 0 | |
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XE63 Xwnt-4 myc-CSKA Resource Report Resource Website |
RRID:Addgene_16876 | Xwnt-4myc | Xenopus laevis | Ampicillin | PMID: | See clone XE2 for details and XE62 for myc tag, and V4 for vector details. Notes for Use: Citation: L. Lynn McGrew, unpublished. | Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:07 | 0 | |
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XE4 Xwnt-5A myc Resource Report Resource Website |
RRID:Addgene_16874 | full coding of Xwnt-5A | Xenopus laevis | Ampicillin | PMID: | Insert: full coding of Xwnt-5A. Insert size: 1.2 Kb. Transcription: linearize with EcoR1 and transcribe with SP6. Plasmid History: Constructed by RTM/JR on the date of 4/93. Notes for Use: This construct encodes full coding sequence of Xwnt-5A, without wnt UTR's. See Xwnt 5A full-length for Genbank data. | Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:07 | 0 | |
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XE174 Xenopus Wnt-5A-myc-pCDbeta-G Resource Report Resource Website |
RRID:Addgene_16873 | Wnt-5A | Xenopus laevis | Ampicillin | PMID: | The myc tagged Xwnt-5A insert of clone XE40, in the SV40 construct pCD-PS modified by Julia Yang-Snyder to include Xenopus beta globin flanking untranslated regions. Reference: The clone was made by Julia Yang-Snyder. Addgene QC found a G125A point mutation that is not known to affect function. | Backbone Marker:Available from Addgene (#17120); Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:07 | 0 | |
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pSP64T-dnWnt-11 Resource Report Resource Website |
RRID:Addgene_16857 | dnWnt-11 | Xenopus laevis | Ampicillin | PMID:10769246 | A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB. For injection, linearize with BamH1 and transcribe with SP6. | Backbone Marker:Available at Addgene (#15030); Backbone Size:3300; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | amino acids 1-282. Dominant Negative. | 2022-04-22 03:32:07 | 0 |
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pCSP1-HA-xEcto Resource Report Resource Website |
RRID:Addgene_20900 | Ectodermin | Xenopus laevis | Ampicillin | PMID:15820681 | For Xenopus embryo mRNA microinjections, cut AscI and transcribe SP6. | Backbone Size:4800; Vector Backbone:pCSP1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains full unsequenced 3' UTR | 2022-04-22 03:35:43 | 0 |
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BAT-gal Resource Report Resource Website |
RRID:Addgene_20889 | 7XTCF binding sites, Siamois minimal promoter, NLS-lacz | Xenopus laevis | Ampicillin | PMID:12626757 | Wnt/beta-catenin responsive promoter in mouse tissues (used to derive the BAT-gal mouse strain), Xenopus embryos and human cell lines | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin | 7 multimerized TCF-binding elements cloned upstream of the xSiamois minimal promoter, driving expression of NLS-lacZ (nuclear localized beta-galactosidase) | 2022-04-22 03:35:43 | 0 |
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BAT-lux Resource Report Resource Website |
RRID:Addgene_20890 | 7xTCF binding sites, Siamois minimal promoter | Xenopus laevis | Ampicillin | PMID:12626757 | Wnt/beta-catenin responsive promoter in Xenopus embryos and human cell lines | Backbone Size:4800; Vector Backbone:pGL3basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 7 multimerized TCF-binding elements fused upstream of the xSiamois minimal promoter | 2022-04-22 03:35:43 | 0 |
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Mix.2-lux M-p53BE Resource Report Resource Website |
RRID:Addgene_20894 | -290 Mix.2 M-p53BE promoter luciferase | Xenopus laevis | Ampicillin | PMID:12732139 | Backbone Size:4800; Vector Backbone:pGL3basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | point mutation of the p53 binding element (p53BE). Bears an additional EcoRI restriction site across the mutated sequence. | 2022-04-22 03:35:43 | 0 | |
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pCS2 xenopus Insulin 2 Resource Report Resource Website |
RRID:Addgene_25786 | Insulin 2 | Xenopus laevis | Ampicillin | PMID:12128218 | Antisense: cut with EcoRI, transcribe with T7 Sense: cut with NotI, transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:37:00 | 0 | |
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pGEM-T-easy Sox17b Resource Report Resource Website |
RRID:Addgene_25782 | Sox 17b | Xenopus laevis | Ampicillin | PMID:10769230 | For antisense RNA cut with ApaI, transcribe with SP6 | Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:37:00 | 0 | |
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pCS2 xenopus sizzled Resource Report Resource Website |
RRID:Addgene_24684 | Sizzled | Xenopus laevis | Ampicillin | PMID:16413488 | Best in situ probe. For anitsense: linearize with NotI Transcribe with SP6 For mRNA injections use 200 pg to dorsalize. E248D does not affect function. | Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:36:45 | 0 | |
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pCS2 xenopus cerberus short Resource Report Resource Website |
RRID:Addgene_24681 | cerberus | Xenopus laevis | Ampicillin | PMID:10067895 | For injection cut with NotI. Transcribe with SP6. The construct fuses aa 1-25 (KHSEG25) to aa 117-270 (117RRKA) | Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Cerberus short | 2022-04-22 03:36:45 | 0 |
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