Plasmids   Select Another Resource Report Type

http://www.addgene.org/91567

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:10690; Vector Backbone:pRK415; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: Please note- 2 mutations in tse2 (H30R and K33Q) were identified during Addgene's quality control process. The depositing lab has noted that these mutations have no affect on plasmid function, and are likely a strain variant. The Pseudomonas aeruginosa PAO1 Tse2 toxin on the backbone acts as a counter selection marker for allelic exchange. Expression of Tse2 is highly toxic to a huge variety of cells, and can result in less background than traditional markers like SacB. See: Khetrapal, Varnica et al. “A Set of Powerful Negative Selection Systems for Unmodified Enterobacteriaceae.” Nucleic Acids Research 43.13 (2015): e83. PMC. Web. 11 Apr. 2017. Toxin expression is tightly repressed by TetR, until induction by anhydrotetracycline. Anhydrotetracycline is non-toxic to most bacteria, and freely crosses membrane barriers without need of specific transporters, potentially enabling this vector to work in a wide variety of bacteria. The ampicillin resistance gene is flanked by two BsaI sites. It can be cleanly removed and replaced with another marker via Golden Gate cloning. If to be used for mating, this plasmid or derivatives can be transformed into commonly used strains such as S17-1λpir, SM10, DH5a(pRK2013), because these strains provide the RK2/RP4 TrfA protein in trans. Important: This suicide plasmid IS NOT based on the R6K gamma ori like most suicide plasmids, so it won't replicate in strains like DH5aλpir.

Proper citation: RRID:Addgene_91567 Copy   

•   Source: Addgene

http://www.addgene.org/187385

Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pKD3; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

Proper citation: RRID:Addgene_187385 Copy   

•   Source: Addgene

http://www.addgene.org/106476

Species: Other
Genetic Insert: ARG1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5189; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_106476 Copy   

•   Source: Addgene

http://www.addgene.org/120275

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Other; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_120275 Copy   

•   Source: Addgene

http://www.addgene.org/124700

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Other
References:
Comments: Genotype: Δasd thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA; integrated RP4-2 Tcr::Mu ΔaphA (λpir+) Strain RHO3 was derived from SM10(λpir+) by deletion of the chromosomal asd gene and the aphA kanamycin resistance determinant located on the chromosomally integrated RP4-2 element (López et al. 2009. Appl. Env. Microbiol. 75:6496-6503).

Proper citation: RRID:Addgene_124700 Copy   

•   Source: Addgene

http://www.addgene.org/125686

Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_125686 Copy   

•   Source: Addgene

http://www.addgene.org/105064

Species:
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Other
References:
Comments: S2060 Genotype: F’ proA+B+ Δ(lacIZY) zzf::Tn10 lacIQ1 PN25-tetR luxCDE Ppsp(AR2) lacZ luxR Plux groESL / endA1 recA1 galE15 galK16 nupG rpsL ΔlacIZYA araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) proBA::pir116 araE201 ΔrpoZ Δflu ΔcsgABCDEFG ΔpgaC λ–

Proper citation: RRID:Addgene_105064 Copy   

•   Source: Addgene

http://www.addgene.org/13333

Species: This is a strain, not a plasmid
Genetic Insert:
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Other
References:
Comments: The strain was derived as follows: a culture of E. coli N99 galK strA was mutagenized with 1-methyl-3'-nitrosoguanidine and Cou-resistant cells were selected on LB agar containing 25ug/mL Cou and 10mM sodium pyrophosphate. These colonies were then screened for failure to grow on LB agar at 42oC. One such strain, N4175, was used as a donor for P1 transduction into N99. The transductant strain, N4177, is Cou-resistant at 32oC and is unable to grow at 42oC. GyrB protein partly purified from N4177 cells grown at 32oC, when added to wild-type GyrA protein, yields a DNA gyrase complex whose supercoiling activity is Cou-resistant at 25oC and temperature sensitive (less than 1% of the activity of the normal enzyme at 42oC).

Proper citation: RRID:Addgene_13333 Copy   

•   Source: Addgene

http://www.addgene.org/172214

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Allelic exchange in Prevotella copri; Bacterial Resistance:Other
References:
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_172214 Copy   

•   Source: Addgene

http://www.addgene.org/172211

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Genetic insertion in Prevotella copri; Bacterial Resistance:Other
References:
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_172211 Copy   

•   Source: Addgene

http://www.addgene.org/110095

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_110095 Copy   

•   Source: Addgene

http://www.addgene.org/70687

Species:
Genetic Insert: Transposon Tn5-1063
Vector Backbone Description: Backbone Marker:Wolk Lab; Vector Backbone:pRL1058; Vector Types:; Bacterial Resistance:Other
References:
Comments: Tn5 derivative Tn5-1063a (in plasmid pRL1063a) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 and pRL1058 is this series of plasmids), providing large numbers of transposon mutants. Plasmid pJE205 bearing the luxA and luxB genes of Vibrio fischeri was the kind gift of M. Silverman, Agouron Institute, La Jolla, CA. The sequence of luxA gene in this construct has been updated and differs slightly from the original X06758.1 sequence. Additional references: Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for the synthesis of heterocyst envelope polysaccharide in Anabaena sp. strain PCC 7120. J Bacteriol 187: 1114-1123 Fan Q, Huang G, Lechno-Yossef S, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for synthesis and deposition of envelope glycolipids in Anabaena sp. strain PCC 7120. Mol Microbiol 58: 227-243

Proper citation: RRID:Addgene_70687 Copy   

•   Source: Addgene

http://www.addgene.org/90098

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10370; Vector Backbone:pPtPBR1; Vector Types:Other, Destination vector for Gateway expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_90098 Copy   

•   Source: Addgene

http://www.addgene.org/187393

Species: Synthetic
Genetic Insert: GFP, mCherry
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).

Proper citation: RRID:Addgene_187393 Copy   

•   Source: Addgene

http://www.addgene.org/110082

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMEXC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_110082 Copy   

•   Source: Addgene

http://www.addgene.org/110078

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMINTC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_110078 Copy   

•   Source: Addgene

http://www.addgene.org/206252

Species: Other
Genetic Insert: H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Other, Recombinant baculovirus production (Bac-to-Bac); Bacterial Resistance:Other
References:
Comments:

Proper citation: RRID:Addgene_206252 Copy   

•   Source: Addgene

http://www.addgene.org/184870

Species: Other
Genetic Insert: lacZalpha,ccdB
Vector Backbone Description: Vector Backbone:Entry vector; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: Please visit https://arxiv.org/pdf/2111.11880.pdf for preprint.

Proper citation: RRID:Addgene_184870 Copy   

•   Source: Addgene

http://www.addgene.org/202275

Species: Synthetic
Genetic Insert: bc140
Vector Backbone Description: Vector Backbone:pBCC091; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202275 Copy   

•   Source: Addgene

http://www.addgene.org/202280

Species: Synthetic
Genetic Insert: bc146
Vector Backbone Description: Vector Backbone:pBCC096; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.

Proper citation: RRID:Addgene_202280 Copy   

•   Source: Addgene