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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAOJ15 Resource Report Resource Website |
RRID:Addgene_91567 | Other | PMID:28614382 | Please note- 2 mutations in tse2 (H30R and K33Q) were identified during Addgene's quality control process. The depositing lab has noted that these mutations have no affect on plasmid function, and are likely a strain variant. The Pseudomonas aeruginosa PAO1 Tse2 toxin on the backbone acts as a counter selection marker for allelic exchange. Expression of Tse2 is highly toxic to a huge variety of cells, and can result in less background than traditional markers like SacB. See: Khetrapal, Varnica et al. “A Set of Powerful Negative Selection Systems for Unmodified Enterobacteriaceae.” Nucleic Acids Research 43.13 (2015): e83. PMC. Web. 11 Apr. 2017. Toxin expression is tightly repressed by TetR, until induction by anhydrotetracycline. Anhydrotetracycline is non-toxic to most bacteria, and freely crosses membrane barriers without need of specific transporters, potentially enabling this vector to work in a wide variety of bacteria. The ampicillin resistance gene is flanked by two BsaI sites. It can be cleanly removed and replaced with another marker via Golden Gate cloning. If to be used for mating, this plasmid or derivatives can be transformed into commonly used strains such as S17-1λpir, SM10, DH5a(pRK2013), because these strains provide the RK2/RP4 TrfA protein in trans. Important: This suicide plasmid IS NOT based on the R6K gamma ori like most suicide plasmids, so it won't replicate in strains like DH5aλpir. | Backbone Marker:N/A; Backbone Size:10690; Vector Backbone:pRK415; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-04-22 03:53:27 | 0 | |||
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pKD3_mCherry Resource Report Resource Website |
RRID:Addgene_187385 | mCherry | Synthetic | Other | PMID: | This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration). | Vector Backbone:pKD3; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2022-09-10 01:02:34 | 0 | |
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pET28a_T5-ARG1 Resource Report Resource Website |
RRID:Addgene_106476 | ARG1 | Other | Other | PMID:29300010 | Backbone Marker:Novagen; Backbone Size:5189; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Other | 2022-04-22 03:16:18 | 0 | ||
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pCRZero Resource Report Resource Website |
RRID:Addgene_120275 | Other | PMID:31015513 | Vector Backbone:pUC18; Vector Types:Other; Bacterial Resistance:Other | 2022-04-22 03:20:37 | 0 | ||||
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RHO3 Resource Report Resource Website |
RRID:Addgene_124700 | Other | PMID:19700544 | Genotype: Δasd thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA; integrated RP4-2 Tcr::Mu ΔaphA (λpir+) Strain RHO3 was derived from SM10(λpir+) by deletion of the chromosomal asd gene and the aphA kanamycin resistance determinant located on the chromosomally integrated RP4-2 element (López et al. 2009. Appl. Env. Microbiol. 75:6496-6503). | Vector Backbone:none; Vector Types:; Bacterial Resistance:Other | 2022-04-22 03:22:02 | 0 | |||
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pCRISPR-Cas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_125686 | streptomyces codon optimized spCas9, sgRNA casette | Other | Other | PMID:25806970 | Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Other | 2022-04-22 03:22:12 | 1 | ||
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S2060 Resource Report Resource Website |
RRID:Addgene_105064 | none | Other | PMID:26258293 | S2060 Genotype: F’ proA+B+ Δ(lacIZY) zzf::Tn10 lacIQ1 PN25-tetR luxCDE Ppsp(AR2) lacZ luxR Plux groESL / endA1 recA1 galE15 galK16 nupG rpsL ΔlacIZYA araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) proBA::pir116 araE201 ΔrpoZ Δflu ΔcsgABCDEFG ΔpgaC λ– | Vector Backbone:none; Vector Types:; Bacterial Resistance:Other | 2022-04-22 03:15:53 | 0 | ||
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N4177 strain Resource Report Resource Website |
RRID:Addgene_13333 | This is a strain, not a plasmid | Other | PMID:6309403 | The strain was derived as follows: a culture of E. coli N99 galK strA was mutagenized with 1-methyl-3'-nitrosoguanidine and Cou-resistant cells were selected on LB agar containing 25ug/mL Cou and 10mM sodium pyrophosphate. These colonies were then screened for failure to grow on LB agar at 42oC. One such strain, N4175, was used as a donor for P1 transduction into N99. The transductant strain, N4177, is Cou-resistant at 32oC and is unable to grow at 42oC. GyrB protein partly purified from N4177 cells grown at 32oC, when added to wild-type GyrA protein, yields a DNA gyrase complex whose supercoiling activity is Cou-resistant at 25oC and temperature sensitive (less than 1% of the activity of the normal enzyme at 42oC). | Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Other | genotype: strA galK gyrB221 (CouR) gyrB203 (ts). CouR mutation is Arg136Cys. ts mutation is Pro171Ser. | 2022-04-22 03:24:49 | 0 | |
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pEx-deletion-tet Resource Report Resource Website |
RRID:Addgene_172214 | Other | PMID:34676563 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. | Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Allelic exchange in Prevotella copri; Bacterial Resistance:Other | 2023-05-05 01:04:34 | 0 | |||
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pEx-insertion-tet Resource Report Resource Website |
RRID:Addgene_172211 | Other | PMID:34676563 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. | Backbone Marker:Koropatkin, N.M. (PMID: 18611383); Backbone Size:4172; Vector Backbone:pExchange-tdk; Vector Types:Other, Genetic insertion in Prevotella copri; Bacterial Resistance:Other | 2023-05-05 01:04:34 | 0 | |||
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pFLAG_attP Resource Report Resource Website |
RRID:Addgene_110095 | Other | PMID:29934571 | Vector Backbone:pFLAG; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2023-05-10 01:00:33 | 0 | ||||
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pRL1063a Resource Report Resource Website |
RRID:Addgene_70687 | Transposon Tn5-1063 | Other | PMID:11607193 | Tn5 derivative Tn5-1063a (in plasmid pRL1063a) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 and pRL1058 is this series of plasmids), providing large numbers of transposon mutants. Plasmid pJE205 bearing the luxA and luxB genes of Vibrio fischeri was the kind gift of M. Silverman, Agouron Institute, La Jolla, CA. The sequence of luxA gene in this construct has been updated and differs slightly from the original X06758.1 sequence. Additional references: Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for the synthesis of heterocyst envelope polysaccharide in Anabaena sp. strain PCC 7120. J Bacteriol 187: 1114-1123 Fan Q, Huang G, Lechno-Yossef S, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for synthesis and deposition of envelope glycolipids in Anabaena sp. strain PCC 7120. Mol Microbiol 58: 227-243 | Backbone Marker:Wolk Lab; Vector Backbone:pRL1058; Vector Types:; Bacterial Resistance:Other | 2023-04-15 01:08:37 | 0 | ||
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pFcpB-DEST Resource Report Resource Website |
RRID:Addgene_90098 | Other | PMID: | Backbone Size:10370; Vector Backbone:pPtPBR1; Vector Types:Other, Destination vector for Gateway expression; Bacterial Resistance:Other | 2023-05-10 01:06:39 | 0 | ||||
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R6K_BAD_GFP-mCherry_ChInt++ Resource Report Resource Website |
RRID:Addgene_187393 | GFP, mCherry | Synthetic | Other | PMID: | This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration). | Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2023-09-15 01:08:42 | 0 | |
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pMEXC3GH (Hyg) Resource Report Resource Website |
RRID:Addgene_110082 | Other | PMID:29934571 | Vector Backbone:pMEXC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2023-09-15 01:01:24 | 0 | ||||
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pMINTC3GH (Apr) Resource Report Resource Website |
RRID:Addgene_110078 | Other | PMID:29934571 | Vector Backbone:pMINTC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2023-09-15 01:01:24 | 0 | ||||
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MultiMate-Rainbow Resource Report Resource Website |
RRID:Addgene_206252 | H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse) | Other | Other | PMID:35801912 | Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Other, Recombinant baculovirus production (Bac-to-Bac); Bacterial Resistance:Other | 2023-10-03 01:05:58 | 0 | ||
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pSWAP_Entry_H2_beta_CM Resource Report Resource Website |
RRID:Addgene_184870 | lacZalpha,ccdB | Other | Other | PMID:35701417 | Please visit https://arxiv.org/pdf/2111.11880.pdf for preprint. | Vector Backbone:Entry vector; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-02-21 12:04:57 | 0 | |
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pFG036 + pFG051 Resource Report Resource Website |
RRID:Addgene_137997 | Tn5 tnp | Synthetic | Other | PMID:32963323 | This plasmid was designed for high density transposon mutagenesis experiment. It can be delivered by conjugation very efficiently for it's transposon to then be inserted in the recipient DNA. The donor strain must have pFG036 as well to avoid unwanted transposition. Indeed, the promoter (pL) in front of the Tn5 transposase encoding gene is repressed by cI expressed from pFG036. The cloning strain must also have pFG036 to be able to maintain pFG051. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions. | Vector Backbone:pFG051; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Other | 2024-08-01 01:02:25 | 0 | |
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pBC113 Resource Report Resource Website |
RRID:Addgene_202275 | bc140 | Synthetic | Other | PMID:38503974 | This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint. | Vector Backbone:pBCC091; Vector Types:Bacterial Expression; Bacterial Resistance:Other | 2024-04-10 01:05:33 | 0 |
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