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http://www.addgene.org/15067

Species: Xenopus laevis
Genetic Insert: BMP receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain.

Proper citation: RRID:Addgene_15067 Copy   

•   Source: Addgene

http://www.addgene.org/15071

Species: Xenopus laevis
Genetic Insert: BMP2-Vg1
Vector Backbone Description: Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Other, Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
References:
Comments: PCR amplified Pro domain of Xenopus BMP2 including tetrabasic cleavage site and 4 a.a. downstream, fused to PCR fragment of Vg1 ligand domain (without first 3 a.a.). See article for more cloning details. The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.

Proper citation: RRID:Addgene_15071 Copy   

•   Source: Addgene

http://www.addgene.org/129771

Species: Xenopus laevis
Genetic Insert: KSP
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_129771 Copy   

•   Source: Addgene

http://www.addgene.org/129772

Species: Xenopus laevis
Genetic Insert: KSP
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: A D6E mutation in KSP is present but is not expected to impact the function of the protein. Requesting scientists should independently validate expected functionality.

Proper citation: RRID:Addgene_129772 Copy   

•   Source: Addgene

http://www.addgene.org/15030

Species: Xenopus laevis
Genetic Insert: beta-globin 5' and 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.

Proper citation: RRID:Addgene_15030 Copy   

•   Source: Addgene

http://www.addgene.org/15010

Species: Xenopus laevis
Genetic Insert: activin-Vg1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TS; Vector Types:Other, Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
References:
Comments: Chimeric activin-Vg1 mRNA, encoding the pro-region of activin βB fused to the mature region of Vg1, resulting in the processing and secretion of mature Vg1. The activin βB-Vg1 hybrid was constructed by PCR amplification of a fragment of a Xenopus activin βB gene encoding the N-terminal pro-region, the tetrabasic cleavage site and the first four amino acids of the C-terminal mature region. This fragment was ligated, in frame, to a PCR amplified fragment of the Vg1 gene encoding the C-terminal mature region lacking the first four amino acids. The ligation product was reamplified, digested at terminal restriction sites and cloned into pSP64TS. The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site. See Author's Map for more information.

Proper citation: RRID:Addgene_15010 Copy   

•   Source: Addgene

http://www.addgene.org/17120

Species: Xenopus laevis
Genetic Insert: beta-globin 5' UTR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-PS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: This is an old but serviceable SV40 expression construct (pCD-PS) modified to give the Xenopus beta-globin untranslated regions and multiple cloning sites from pT7TS vector (Addgene plasmid 17091). Reference: The vector was made by Julia Yang-Snyder.

Proper citation: RRID:Addgene_17120 Copy   

•   Source: Addgene

http://www.addgene.org/17146

Species: Xenopus laevis
Genetic Insert: Beta-tubulin promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4300; Vector Backbone:pBLCAT3; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: Constructed Rebecca Beach and Paul A. Kreig. 3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment.

Proper citation: RRID:Addgene_17146 Copy   

•   Source: Addgene

http://www.addgene.org/17147

Species: Xenopus laevis
Genetic Insert: NCAM promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(+); Vector Types:Other, Xenopus expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid History: Constructed Paul A. Kreig, July. Note for Use: This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence.

Proper citation: RRID:Addgene_17147 Copy   

•   Source: Addgene

http://www.addgene.org/171548

Species: Xenopus laevis
Genetic Insert: Xlrbpa
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_171548 Copy   

•   Source: Addgene

http://www.addgene.org/171547

Species: Xenopus laevis
Genetic Insert: Xlrbpa
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_171547 Copy   

•   Source: Addgene

http://www.addgene.org/17158

Species: Xenopus laevis
Genetic Insert: goosecoid promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Generated by PCR using the 3' primer (nucleotides - 5 to + 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into pOLuc.

Proper citation: RRID:Addgene_17158 Copy   

•   Source: Addgene

http://www.addgene.org/17159

Species: Xenopus laevis
Genetic Insert: goosecoid promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987).

Proper citation: RRID:Addgene_17159 Copy   

•   Source: Addgene

http://www.addgene.org/16307

Species: Xenopus laevis
Genetic Insert: Axin GID 1-2
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_16307 Copy   

•   Source: Addgene

http://www.addgene.org/16305

Species: Xenopus laevis
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense mRNA cut with NotI and transcribe with SP6

Proper citation: RRID:Addgene_16305 Copy   

•   Source: Addgene

http://www.addgene.org/16302

Species: Xenopus laevis
Genetic Insert: Axin GID 5-6 LP
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: GID=Axin GSK3 interaction domain. L->P is an inactive control. It does not bind GSK-3 and does not inhibit GSK-3. For sense transcripts, cut with NotI.

Proper citation: RRID:Addgene_16302 Copy   

•   Source: Addgene

http://www.addgene.org/16301

Species: Xenopus laevis
Genetic Insert: Axin GID 5-6
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: GID=Axin GSK3 interaction domain. GID5-6 is a smaller version than GID1-2. These constructs bind and inhibit GSK-3 and potently activate Wnt signaling. For sense transcripts, cut with NotI and transcribe with SP6

Proper citation: RRID:Addgene_16301 Copy   

•   Source: Addgene

http://www.addgene.org/16321

Species: Xenopus laevis
Genetic Insert: frizzled-8
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI.

Proper citation: RRID:Addgene_16321 Copy   

•   Source: Addgene

http://www.addgene.org/16314

Species: Xenopus laevis
Genetic Insert: Kermit 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6

Proper citation: RRID:Addgene_16314 Copy   

•   Source: Addgene

http://www.addgene.org/16329

Species: Xenopus laevis
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: Secreted Wnt inhibitor. For sense transcripts, cut with NotI and transcribe with SP6. Please note that the cloning strategy converted phenylalanine-229 to a tyrosine (F229Y), just before the termination codon. According to the depositing scientist, this change is unlikely to affect function.

Proper citation: RRID:Addgene_16329 Copy   

•   Source: Addgene