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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pSP64T tBMPR (DM#135) Resource Report Resource Website |
RRID:Addgene_15067 | BMP receptor | Xenopus laevis | Ampicillin | PMID:7522972 | To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain. | Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Truncated dominant negative form | 2022-04-22 03:27:56 | 0 |
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pSP64T3 BMP2 pro Vg1 (DM#119) Resource Report Resource Website |
RRID:Addgene_15071 | BMP2-Vg1 | Xenopus laevis | Ampicillin | PMID:8348610 | PCR amplified Pro domain of Xenopus BMP2 including tetrabasic cleavage site and 4 a.a. downstream, fused to PCR fragment of Vg1 ligand domain (without first 3 a.a.). See article for more cloning details. The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA. | Backbone Marker:Modified form of pSP64T that contains KpnI & XhoI near BglII; Backbone Size:3000; Vector Backbone:pSP64T3; Vector Types:Other, Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin | 2022-04-22 03:27:56 | 0 | |
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KSP_K406 Resource Report Resource Website |
RRID:Addgene_129771 | KSP | Xenopus laevis | Ampicillin | PMID: | Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Truncation at 367, fused to kinesin-1 starting Ala345 | 2022-04-22 03:23:41 | 0 | |
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KSP_HNL Resource Report Resource Website |
RRID:Addgene_129772 | KSP | Xenopus laevis | Ampicillin | PMID: | A D6E mutation in KSP is present but is not expected to impact the function of the protein. Requesting scientists should independently validate expected functionality. | Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Truncation at 367 | 2022-04-22 03:23:41 | 0 |
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pSP64T (DM#111) Resource Report Resource Website |
RRID:Addgene_15030 | beta-globin 5' and 3' UTR | Xenopus laevis | Ampicillin | PMID:6207484 | Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information. | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:27:56 | 0 | |
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pSP64TS Activin pro Vg1 Flag (DM#171) Resource Report Resource Website |
RRID:Addgene_15010 | activin-Vg1 | Xenopus laevis | Ampicillin | PMID:8797821 | Chimeric activin-Vg1 mRNA, encoding the pro-region of activin βB fused to the mature region of Vg1, resulting in the processing and secretion of mature Vg1. The activin βB-Vg1 hybrid was constructed by PCR amplification of a fragment of a Xenopus activin βB gene encoding the N-terminal pro-region, the tetrabasic cleavage site and the first four amino acids of the C-terminal mature region. This fragment was ligated, in frame, to a PCR amplified fragment of the Vg1 gene encoding the C-terminal mature region lacking the first four amino acids. The ligation product was reamplified, digested at terminal restriction sites and cloned into pSP64TS. The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site. See Author's Map for more information. | Backbone Size:3000; Vector Backbone:pSP64TS; Vector Types:Other, Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin | 2022-04-22 03:27:56 | 0 | |
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V26 pCD-betaG-PS Resource Report Resource Website |
RRID:Addgene_17120 | beta-globin 5' UTR | Xenopus laevis | Ampicillin | PMID: | Description: This is an old but serviceable SV40 expression construct (pCD-PS) modified to give the Xenopus beta-globin untranslated regions and multiple cloning sites from pT7TS vector (Addgene plasmid 17091). Reference: The vector was made by Julia Yang-Snyder. | Backbone Size:0; Vector Backbone:pCD-PS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:42 | 0 | |
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3.8NBetaT-CAT Resource Report Resource Website 1+ mentions |
RRID:Addgene_17146 | Beta-tubulin promoter | Xenopus laevis | Ampicillin | PMID: | Constructed Rebecca Beach and Paul A. Kreig. 3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment. | Backbone Marker:ATCC; Backbone Size:4300; Vector Backbone:pBLCAT3; Vector Types:; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:47 | 1 | |
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M10 NCAM Promoter Resource Report Resource Website |
RRID:Addgene_17147 | NCAM promoter | Xenopus laevis | Ampicillin | PMID: | Plasmid History: Constructed Paul A. Kreig, July. Note for Use: This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(+); Vector Types:Other, Xenopus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:48 | 0 | |
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pGEX-4T-1-Xlrbpa-dsRBD(+6) Resource Report Resource Website |
RRID:Addgene_171548 | Xlrbpa | Xenopus laevis | Ampicillin | PMID:33751023 | Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | a six amino acid_TRLTEG_insertion in the L1 region | 2022-04-22 03:32:49 | 0 | |
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pGEX-4T-1-Xlrbpa Resource Report Resource Website |
RRID:Addgene_171547 | Xlrbpa | Xenopus laevis | Ampicillin | PMID:33751023 | Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:49 | 0 | ||
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-226bp goosecoid luciferase Resource Report Resource Website |
RRID:Addgene_17158 | goosecoid promoter | Xenopus laevis | Ampicillin | PMID:8543150 | Generated by PCR using the 3' primer (nucleotides - 5 to + 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into pOLuc. | Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:50 | 0 | |
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-1.5 kb goosecoid luciferase Resource Report Resource Website |
RRID:Addgene_17159 | goosecoid promoter | Xenopus laevis | Ampicillin | PMID:8543150 | The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987). | Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:50 | 0 | |
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Axin GID 1-2/pET29b Resource Report Resource Website |
RRID:Addgene_16307 | Axin GID 1-2 | Xenopus laevis | Kanamycin | PMID:10490650 | Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Amino acids 320-429 | 2022-04-22 03:30:47 | 0 | |
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Axin/pCS2HA Resource Report Resource Website |
RRID:Addgene_16305 | Axin | Xenopus laevis | Ampicillin | PMID:10072781 | For sense mRNA cut with NotI and transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:46 | 0 | |
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Axin GID 5-6 LP/pCS2MT Resource Report Resource Website |
RRID:Addgene_16302 | Axin GID 5-6 LP | Xenopus laevis | Ampicillin | PMID:10490650 | GID=Axin GSK3 interaction domain. L->P is an inactive control. It does not bind GSK-3 and does not inhibit GSK-3. For sense transcripts, cut with NotI. | Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | Amino acids 380-404, L to P mutation | 2022-04-22 03:30:46 | 0 |
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Axin GID 5-6/pCS2MT Resource Report Resource Website |
RRID:Addgene_16301 | Axin GID 5-6 | Xenopus laevis | Ampicillin | PMID:10490650 | GID=Axin GSK3 interaction domain. GID5-6 is a smaller version than GID1-2. These constructs bind and inhibit GSK-3 and potently activate Wnt signaling. For sense transcripts, cut with NotI and transcribe with SP6 | Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | GSK-3 interaction domain 380-404 | 2022-04-22 03:30:45 | 0 |
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xfz8/pCS2+ Resource Report Resource Website |
RRID:Addgene_16321 | frizzled-8 | Xenopus laevis | Ampicillin | PMID:9636083 | For sense transcripts, cut with NotI. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:49 | 0 | |
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kermit-2/pCS2+ Resource Report Resource Website |
RRID:Addgene_16314 | Kermit 2 | Xenopus laevis | Ampicillin | PMID:16914488 | For sense transcripts, cut with NotI and transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:48 | 0 | |
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xfz2N/pCS2+ Resource Report Resource Website |
RRID:Addgene_16329 | frizzled-2 | Xenopus laevis | Ampicillin | PMID:10495293 | Secreted Wnt inhibitor. For sense transcripts, cut with NotI and transcribe with SP6. Please note that the cloning strategy converted phenylalanine-229 to a tyrosine (F229Y), just before the termination codon. According to the depositing scientist, this change is unlikely to affect function. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | Extracellular ligand binding domain of Xenopus frizzled-2. | 2022-04-22 03:30:51 | 0 |
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