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Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates.
The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long
Proper citation: RRID:Addgene_45937 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates.
The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long
Proper citation: RRID:Addgene_45939 Copy
Species: Homo sapiens
Genetic Insert: SAE1
Vector Backbone Description: Backbone Marker:Novagen - lab stock; Backbone Size:3600; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_175241 Copy
Species: Other
Genetic Insert: PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_175579 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
References:
Comments: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA]
Proper citation: RRID:Addgene_24545 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4553; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_31288 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pHAtC; Vector Types:Plant Expression, Other, Plant Binary vector; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_169765 Copy
Species: Other
Genetic Insert: HIV-2 RT p66 (mutant)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3498; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint
Proper citation: RRID:Addgene_170279 Copy
Species: Other
Genetic Insert: Bst-LF D720A
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3543; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint
Proper citation: RRID:Addgene_170277 Copy
Species: Synthetic
Genetic Insert: LuxR-mRFP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:original Duet vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_172557 Copy
Species: Homo sapiens
Genetic Insert: Replication factor C subunit 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_175043 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_49796 Copy
Species:
Genetic Insert: genotype: rpsL, thr, leu, thi, lacY, galK, galT, ara, tomA, tsx, dam, dcm, supE44, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_49763 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6798; Vector Backbone:see comments; Vector Types:Other, Conditionally lethal; Bacterial Resistance:Streptomycin
References:
Comments: sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921).
The streptomycin/spectinomycin determinant in pRL277 was derived from the omega cassette (Prentki, et al. Gene 103: 17-23 1991).
Additional Reference for this plasmid:
Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153
Proper citation: RRID:Addgene_70255 Copy
Species: Other
Genetic Insert: formylglycinamidine ribonucleotide synthetase, small subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: pCloDF13 replicon
Proper citation: RRID:Addgene_72516 Copy
Species: Other
Genetic Insert: formylglycinamidine ribonucleotide synthetase, small subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: pCloDF13 replicon
Proper citation: RRID:Addgene_72517 Copy
Species: Other
Genetic Insert: formylglycinamidine ribonucleotide synthetase, large subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: pCloDF13 replicon
Proper citation: RRID:Addgene_72520 Copy
Species: Other
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: CDF ori
Proper citation: RRID:Addgene_72450 Copy
Species: Other
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide synthetase
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments: CDF ori
Proper citation: RRID:Addgene_72449 Copy
Species: Other
Genetic Insert: Cas1, Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_72676 Copy
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