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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pTD-NTwinStrep_Sm Resource Report Resource Website |
RRID:Addgene_45937 | Streptomycin | PMID:23687945 | This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long | Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:41:30 | 0 | |||
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pTD-CTwinStrep Resource Report Resource Website |
RRID:Addgene_45939 | Streptomycin | PMID:23687945 | This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long | Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:41:30 | 0 | |||
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SAE1 Resource Report Resource Website |
RRID:Addgene_175241 | SAE1 | Homo sapiens | Streptomycin | PMID:34035521 | Backbone Marker:Novagen - lab stock; Backbone Size:3600; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:33:34 | 0 | ||
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pQCascade(Ptr)-entry(BsaI) Resource Report Resource Website |
RRID:Addgene_175579 | PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI) | Other | Streptomycin | PMID:34478496 | Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | NO | 2022-04-22 03:33:40 | 0 | |
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MW005 (bacterial strain) Resource Report Resource Website |
RRID:Addgene_24545 | Streptomycin | PMID:20350301 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA] | Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin | 2022-04-22 03:36:42 | 0 | |||
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pTCS-mcs Resource Report Resource Website |
RRID:Addgene_31288 | Streptomycin | PMID:21238944 | Backbone Size:4553; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:38:14 | 0 | ||||
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pECO200 Resource Report Resource Website |
RRID:Addgene_169765 | Streptomycin | PMID:32190101 | Vector Backbone:pHAtC; Vector Types:Plant Expression, Other, Plant Binary vector; Bacterial Resistance:Streptomycin | 2022-04-22 03:32:17 | 0 | ||||
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pGV3341 Resource Report Resource Website |
RRID:Addgene_170279 | HIV-2 RT p66 (mutant) | Other | Streptomycin | PMID:34082799 | Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint | Backbone Marker:Novagen; Backbone Size:3498; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:32:26 | 0 | |
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pGV3319 Resource Report Resource Website |
RRID:Addgene_170277 | Bst-LF D720A | Other | Streptomycin | PMID:34082799 | Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint | Backbone Marker:Novagen; Backbone Size:3543; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | D720A | 2022-04-22 03:32:26 | 0 |
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pCLxR6 Resource Report Resource Website |
RRID:Addgene_172557 | LuxR-mRFP | Synthetic | Streptomycin | PMID: | Backbone Marker:Novagen; Vector Backbone:original Duet vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin | 2022-04-22 03:33:03 | 0 | ||
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pCDF-1b-RFC140ΔN555 Resource Report Resource Website |
RRID:Addgene_175043 | Replication factor C subunit 1 | Homo sapiens | Streptomycin | PMID:32907938 | Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:33:31 | 0 | ||
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pCDM4 Resource Report Resource Website |
RRID:Addgene_49796 | Streptomycin | PMID:23651248 | Backbone Marker:Novagen; Backbone Size:5000; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:42:32 | 0 | ||||
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JM110 Resource Report Resource Website |
RRID:Addgene_49763 | genotype: rpsL, thr, leu, thi, lacY, galK, galT, ara, tomA, tsx, dam, dcm, supE44, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15] | Streptomycin | PMID:2985470 | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Streptomycin | 2022-04-22 03:42:32 | 0 | |||
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pRL277 Resource Report Resource Website |
RRID:Addgene_70255 | Streptomycin | PMID:2160938 | sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The streptomycin/spectinomycin determinant in pRL277 was derived from the omega cassette (Prentki, et al. Gene 103: 17-23 1991). Additional Reference for this plasmid: Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153 | Backbone Size:6798; Vector Backbone:see comments; Vector Types:Other, Conditionally lethal; Bacterial Resistance:Streptomycin | 2022-04-22 03:47:44 | 0 | |||
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pJK543 Resource Report Resource Website |
RRID:Addgene_72516 | formylglycinamidine ribonucleotide synthetase, small subunit | Other | Streptomycin | PMID: | pCloDF13 replicon | Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | contains additional Ala2 | 2022-04-22 03:48:22 | 0 |
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pJK544 Resource Report Resource Website |
RRID:Addgene_72517 | formylglycinamidine ribonucleotide synthetase, small subunit | Other | Streptomycin | PMID: | pCloDF13 replicon | Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | contains additional Ala2 | 2022-04-22 03:48:22 | 0 |
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pJK549 Resource Report Resource Website |
RRID:Addgene_72520 | formylglycinamidine ribonucleotide synthetase, large subunit | Other | Streptomycin | PMID: | pCloDF13 replicon | Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:48:22 | 0 | |
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pJK550 Resource Report Resource Website |
RRID:Addgene_72450 | N5-carboxyaminoimidazole ribonucleotide mutase | Other | Streptomycin | PMID: | CDF ori | Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | inserted Val2 | 2022-04-22 03:48:21 | 0 |
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pJK547 Resource Report Resource Website |
RRID:Addgene_72449 | N5-carboxyaminoimidazole ribonucleotide synthetase | Other | Streptomycin | PMID: | CDF ori | Backbone Size:3781; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | Val319 to Phe | 2022-04-22 03:48:21 | 0 |
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pCas1+2 Resource Report Resource Website |
RRID:Addgene_72676 | Cas1, Cas2 | Other | Streptomycin | PMID:22402487 | Backbone Marker:Novagen; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | 2022-04-22 03:48:25 | 0 |
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