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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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JM101 Resource Report Resource Website |
RRID:Addgene_50349 | Relevant genotype: supE, thi, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15] | Other | None | PMID: | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:19 | 0 | ||
|
JM83 Resource Report Resource Website |
RRID:Addgene_50348 | Relevant genotype: ara, Δ(lac-proAB), rspL(+strA), ϕ80, lacZΔM15 | Other | None | PMID: | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:19 | 0 | ||
|
HL 865 Resource Report Resource Website |
RRID:Addgene_60371 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ dsrA | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:59:29 | 0 | ||
|
HL 862 Resource Report Resource Website |
RRID:Addgene_60370 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ micC | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:59:29 | 0 | ||
|
HL 1128 Resource Report Resource Website |
RRID:Addgene_60372 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ hfq + Δ micC | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:59:29 | 0 | ||
|
BL21ΔBC Resource Report Resource Website |
RRID:Addgene_102263 | None | PMID:29164072 | Genotype = ΔlamB ΔompC Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
BL21ΔCF Resource Report Resource Website |
RRID:Addgene_102265 | None | PMID:29164072 | Genotype = ΔompC ΔompF Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
BL21ΔABC Resource Report Resource Website |
RRID:Addgene_102266 | None | PMID:29164072 | Genotype = ΔompA ΔlamB ΔompC Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
BL21ΔAF Resource Report Resource Website |
RRID:Addgene_102262 | None | PMID:29164072 | Genotype = ΔompA ΔompF Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
BL21ΔABF Resource Report Resource Website |
RRID:Addgene_102267 | None | PMID:29164072 | Genotype = ΔompA ΔlamB ΔompF Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
pSELECT-HA-mFOXO1-D256 Resource Report Resource Website |
RRID:Addgene_83379 | Forkhead box O1 | Mus musculus | None | PMID: | Backbone Marker:Invivogen; Backbone Size:3390; Vector Backbone:pSELECT-puro; Vector Types:Mammalian Expression; Bacterial Resistance:None | N256TAG (premature stop codon) | 2023-04-15 01:09:22 | 0 | |
|
BL21ΔA Resource Report Resource Website |
RRID:Addgene_102256 | None | PMID:29164072 | Genotype = ΔompA Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
BL21ΔB Resource Report Resource Website |
RRID:Addgene_102257 | None | PMID:29164072 | Genotype = ΔlamB Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-15 01:00:16 | 0 | |||
|
HL 3338 Resource Report Resource Website |
RRID:Addgene_60376 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ hfq + Δ ryhB | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:34 | 0 | ||
|
RF4 Resource Report Resource Website |
RRID:Addgene_62072 | none | None | PMID:26577727 | Genotype = aspC tyrB Precursor strain = RF3 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
RF13 Resource Report Resource Website |
RRID:Addgene_62078 | none | None | PMID:26577727 | Genotype = aspC tyrB trpA trpB Precursor strain = RF4 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, Trp, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
HL 2752 Resource Report Resource Website |
RRID:Addgene_60374 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ ryhB | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-14 01:07:34 | 0 | ||
|
RF6 Resource Report Resource Website |
RRID:Addgene_62074 | none | None | PMID:26577727 | Genotype = proC Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Pro Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
RF8 Resource Report Resource Website |
RRID:Addgene_62075 | none | None | PMID:26577727 | Genotype = asnA asnB Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asn Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:39 | 0 | ||
|
YM138 Resource Report Resource Website |
RRID:Addgene_61953 | none | None | PMID:26577727 | Genotype = cysE Precursor strain = C43(DE3) Selective amino acid labeling (and/or requirement) = Cys Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2023-04-14 01:07:38 | 0 |
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