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Species: Homo sapiens
Genetic Insert: Sequestosome1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mVenus-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 515; Emission = 528
Proper citation: RRID:Addgene_56335 Copy
Species: Homo sapiens
Genetic Insert: SARA
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67347 Copy
Species: Homo sapiens
Genetic Insert: SAR1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67344 Copy
Species: Homo sapiens
Genetic Insert: SAR1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67345 Copy
Species: Homo sapiens
Genetic Insert: CMKLR1
Vector Backbone Description: Backbone Size:6632; Vector Backbone:empty Tango; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Most of the PRESTO-Tango vectors do not contain the XhoI cut site downstream of the tTA sequence and instead carry an XbaI site. If you need to perform an XhoI digest we recommend sequencing with a forward primer at the C-terminus of tTA to determine which of the two sites is present. Suggested primer: 5-gagctccacttagacggcgagg-3. Original backbone was pcDNA3.1(+). These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_66253 Copy
Species: Homo sapiens
Genetic Insert: ARFRP1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67342 Copy
Species: Homo sapiens
Genetic Insert: Sequestosome1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55132 Copy
Species: Homo sapiens
Genetic Insert: ARF4
Vector Backbone Description: Vector Backbone:pDS_Myc-X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that this plasmid contains the V53A, L123P and M134I mutations in ARF4 from the parent vector pDONR221-ARF4-no stop (Addgene plasmid # 67311). These mutations were unintentionally introduced during PCR.
Proper citation: RRID:Addgene_67350 Copy
Species: Homo sapiens
Genetic Insert: ARL4
Vector Backbone Description: Vector Backbone:pDS_Myc-X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67356 Copy
Species: Homo sapiens
Genetic Insert: ARL8B
Vector Backbone Description: Vector Backbone:pDS_Myc-X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67362 Copy
Species: Homo sapiens
Genetic Insert: ARL8A
Vector Backbone Description: Vector Backbone:pDS_Myc-X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67361 Copy
Species: Homo sapiens
Genetic Insert: ARL11
Vector Backbone Description: Vector Backbone:pDS_Myc-X; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67365 Copy
Species: Mus musculus
Genetic Insert: MarsL274G
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Developed by Alborz Mahdavi.
Proper citation: RRID:Addgene_63177 Copy
Species: Homo sapiens
Genetic Insert: collagen, type IV, alpha 3 binding protein PH domain
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: PH domain of CERT= Methionine 1 - Glycine 122
Proper citation: RRID:Addgene_58833 Copy
Species: Homo sapiens
Genetic Insert: ARF3
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67309 Copy
Species: Homo sapiens
Genetic Insert: ARF1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67306 Copy
Species: Homo sapiens
Genetic Insert: ARF1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67307 Copy
Species: Mus musculus
Genetic Insert: Tiam1-pMag(3x)-iRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5354; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67305 Copy
Species: Homo sapiens
Genetic Insert: ARL1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA-DEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67395 Copy
Species: Homo sapiens
Genetic Insert: ARL2
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA-DEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67396 Copy
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