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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCAGGS-hPhyB-mCherry-HRasCT
 
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RRID:Addgene_100281 Phytochrome B Arabidopsis thaliana Ampicillin PMID:29078307 Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio. mCherry has a G229L mutation that does not affect function. Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Human codon optimized PhyB (1-908 aa) 2022-04-22 03:14:48 0
pCAGGS-PHFF
 
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RRID:Addgene_100280 MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes) Other Ampicillin PMID:29078307 Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin All genes are codon-optimized for expression in human cells. 2022-04-22 03:14:48 0
pCAGGS-PHFF-sh-mBVRA
 
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RRID:Addgene_100286 MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes) Other Ampicillin PMID:29078307 Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to mouse BVRA is expressed so that PCB is further accumulated. Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin All genes are codon-optimized for expression in human cells. 2022-04-22 03:14:49 0
pCAGGS-PHFF-sh-hBVRA
 
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RRID:Addgene_100285 MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes) Other Ampicillin PMID:29078307 Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to human BVRA is expressed so that PCB is further accumulated. Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin All genes are codon-optimized for expression in human cells. 2022-04-22 03:14:49 0
pLM494
 
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RRID:Addgene_100539 GGPP synthase Other Ampicillin PMID:29789561 Addgene NGS sequencing found the following mutations compared to the provided reference sequence: crtE with M176I, K346R, I347M, E353D mutations, crtI with L107F, H427Q mutations, and crtYB with A190T, E307K mutations. tHMG1 contains only residues 531-1054 compared to the NCBI reference seq [M22002.1]. These mutations/truncation do not affect plasmid function. Backbone Size:4846; Vector Backbone:pLM304; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin 2022-04-22 03:14:52 0
pLH_Scr15
 
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RRID:Addgene_100537 Phytochrome B Arabidopsis thaliana Ampicillin PMID:29789561 Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin N-terminal version of PhyB (AA 1-621)/ Mutation of nucleotide 576 C-->A 2022-04-22 03:14:52 0
pFloxed DHFR-TS*
 
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RRID:Addgene_100605 Floxed TgDHFR-TS* Other Ampicillin PMID: Backbone Size:2678; Vector Backbone:pUC19; Vector Types:Other, Toxoplasma expression; Bacterial Resistance:Ampicillin Pyrimethamine resistance 2022-04-22 03:14:53 0
pEF1a-FB-dCas9-puro
 
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1+ mentions
RRID:Addgene_100547 N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 Other Ampicillin PMID:28841410 Backbone Size:7064; Vector Backbone:pEF1a-FB-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2022-04-22 03:14:52 1
pcDNA3.1-TK1
 
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RRID:Addgene_100544 thymidine kinase 1 Homo sapiens Ampicillin PMID:29100421 Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:14:52 0
ciCas9(F22)_pcDNA5
 
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RRID:Addgene_100552 ciCas9(F22) Ampicillin PMID:28737741 Backbone Marker:Life Technology; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, CRISPR, Other, FRT, Flp-In; Bacterial Resistance:Ampicillin 2022-04-22 03:14:52 0
ciCas9(L22)_pcDNA5
 
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RRID:Addgene_100551 ciCas9(L22) Ampicillin PMID:28737741 Backbone Marker:Life Technology; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, CRISPR, Other, FRT, Flp-In; Bacterial Resistance:Ampicillin 2022-04-22 03:14:52 0
TERTp146_Gluc_CCR5
 
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RRID:Addgene_192272 Promoter Reporter Insert within donor template Homo sapiens Ampicillin PMID:34010660 Backbone Marker:Manuel A.F.V. Goncalves; Vector Backbone:AY10_pS.Donor.R5.TS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin -146 C to T 2022-12-22 03:25:31 0
pMSCV puro PRDM14 ΔSET+ΔZinc finger domains
 
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RRID:Addgene_193678 PRDM14 Homo sapiens Ampicillin PMID:34990589 Vector Backbone:pMSCV puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin Deletion of SET and Zinc finger domains 2022-12-22 03:26:00 0
1D3 HC
 
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RRID:Addgene_170670 HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:36378644 Predicted peptide sequences for 1D3 Heavy Chain: MDFGLRLIFLVLVFKGVLCQVQLQQPGAELVKPGASVKMSCKASGYTFTSNNMHWVKQTPGQGLEWIGGLYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSGDSAVYYCARGIRDFFAMDYWGQGTSVTVSSASLTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSLSPGK* Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-22 02:15:19 0
1D3 LC
 
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RRID:Addgene_170669 LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:36378644 Predicted peptide sequences for 1D3 Light Chain: MKFPSQLLLLLLFGIPGMRSDIQMTQSPASQSASLGESVTITCLASQTIGTWLAWYQQKPGKSPQLLIYAATSLADGVPSRFSGSGSGTKFSFKISSLQAEDFVSYYCQQLYSTPLTFGAGTKLELKRAAAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC* Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-22 02:15:19 0
pEXP1-DEST-Noa1-HIS
 
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RRID:Addgene_191972 Noa1-HIS Homo sapiens Ampicillin PMID: Vector Backbone:pEXP1-DEST; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2022-12-22 02:16:00 0
pSems-leader-mXFPm-IFNGR1(18-489)
 
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RRID:Addgene_192786 IFNGR1 Homo sapiens Ampicillin PMID:35474965 Backbone Marker:Covalys, NEB; Backbone Size:5787; Vector Backbone:pSEMS(26m); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin mXFPm: tryptophan 66 to phenylalanine, gluatmic acid 142 to asparagine and histidine 164 to asparagine 2022-12-22 02:16:01 0
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
 
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RRID:Addgene_192784 IFNAR1 Homo sapiens Ampicillin PMID:35474965 Backbone Marker:Covalys, NEB; Backbone Size:5787; Vector Backbone:pSEMS(26m); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin mXFPm: tryptophan 66 to phenylalanine, gluatmic acid 142 to asparagine and histidine 164 to asparagine 2022-12-22 02:16:01 0
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6
 
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RRID:Addgene_192788 antiGFP nanobody enhancer Other Ampicillin PMID:35474965 Reference to anti-GFP nanobody enhancer: Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U. Modulation of protein properties in living cells using nanobodies. Nat Struct Mol Biol. 2010 Jan;17(1):133-8. doi: 10.1038/nsmb.1727. Epub 2009 Dec 13. PMID: 20010839. Backbone Marker:EMD Biosciences; Backbone Size:5443; Vector Backbone:pet21a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-12-22 02:16:01 0
pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo
 
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RRID:Addgene_191397 sgRNA targeting alpha satellites on human chromosome 9 Homo sapiens Ampicillin PMID:28935858 Backbone Size:7100; Vector Backbone:pSIN-U6-tracer_sgRNA_-EF1a-Thy1.1-P2A-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2022-12-22 02:15:59 0

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