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Species: Synthetic
Genetic Insert: 3 miR-122 target sites
Vector Backbone Description: Vector Backbone:pAAVf-EnhCB-lacZnls; Vector Types:AAV, Other; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_35644 Copy
Species: Other
Genetic Insert: 2QG5
Vector Backbone Description: Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/2QG5/
Proper citation: RRID:Addgene_51331 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38195 Copy
Species: Other
Genetic Insert: 3L43
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/3L43/
Proper citation: RRID:Addgene_51334 Copy
Species: Other
Genetic Insert: 3CBQ
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/3CBQ/
Proper citation: RRID:Addgene_51337 Copy
Species: Synthetic
Genetic Insert: TuD miR-122
Vector Backbone Description: Backbone Size:4903; Vector Backbone:pAAVscCBPIGpluc7xmiR-122BT; Vector Types:AAV, Other; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_35649 Copy
Species: Other
Genetic Insert: 2LV9
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/2LV9/
Proper citation: RRID:Addgene_51343 Copy
Species: Other
Genetic Insert: 2ONU
Vector Backbone Description: Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/2ONU/
Proper citation: RRID:Addgene_51346 Copy
Species: Homo sapiens
Genetic Insert: SND1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: PDB:
http://www.thesgc.org/structures/3OMC/
http://www.thesgc.org/structures/3OMG/
Proper citation: RRID:Addgene_28159 Copy
Species: Other
Genetic Insert: 2I81
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/2I81/
Proper citation: RRID:Addgene_51352 Copy
Species: Other
Genetic Insert: 2QG7
Vector Backbone Description: Vector Backbone:p15TV-L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/2QG7/
Proper citation: RRID:Addgene_51351 Copy
Species: Homo sapiens
Genetic Insert: 2Q3F
Vector Backbone Description: Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/2Q3F/
Proper citation: RRID:Addgene_51356 Copy
Species: Other
Genetic Insert: 3CKL
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/3CKL/
Proper citation: RRID:Addgene_51359 Copy
Species: Homo sapiens
Genetic Insert: SLC7A14
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45261 Copy
Species: Mus musculus
Genetic Insert: CIP4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned from IMAGE clone: 3500118 (MGC:6673).
Proper citation: RRID:Addgene_27685 Copy
Species: Homo sapiens
Genetic Insert: GPBP
Vector Backbone Description: Backbone Marker:c; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: PH binding domain of GPBP recognizes PI4P
Proper citation: RRID:Addgene_49572 Copy
Species: Homo sapiens
Genetic Insert: SSTR3
Vector Backbone Description: Backbone Size:6632; Vector Backbone:empty Tango; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Most of the PRESTO-Tango vectors do not contain the XhoI cut site downstream of the tTA sequence and instead carry an XbaI site. If you need to perform an XhoI digest we recommend sequencing with a forward primer at the C-terminus of tTA to determine which of the two sites is present. Suggested primer: 5-gagctccacttagacggcgagg-3. Original backbone was pcDNA3.1(+). These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_66504 Copy
Species: Homo sapiens
Genetic Insert: ARL2
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Other, Gateway Entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67318 Copy
Species: Other
Genetic Insert: L-IFP-F[2]
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLpC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52902 Copy
Species: Other
Genetic Insert: crtE, crtY, crtI
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Other, low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
References:
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53282 Copy
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