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Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101167 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/E60C/D273C
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101168 Copy
Species: Other
Genetic Insert: SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101203 Copy
Species: Homo sapiens
Genetic Insert: Cox8A
Vector Backbone Description: Backbone Size:6077; Vector Backbone:pCLIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Originally cloned by Covalys. The CLIP-tag is an C-insert tag. Additional antibiotic resistance to Kanmycin
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101130 Copy
Species: Other
Genetic Insert: GPI
Vector Backbone Description: Backbone Size:5151; Vector Backbone:pMCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + MCP+GPI is 408 bp. The MCP-tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin.
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101132 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5802; Vector Backbone:pSNAP-tag (m) Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Codon usage of the gene is optimized for expression in mammalian cells. pSNAP-tag(m) contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101135 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3725; Vector Backbone:pSNAP-tag (T7) Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: pSNAP-tag(T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the gene is optimized for expression in E. coli. The fusion partner should be cloned as a fusion to the C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101137 Copy
Species: Other
Genetic Insert: LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5104; Vector Backbone:pCBG68 Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101198 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101192 Copy
Species:
Genetic Insert: ffDronpa-F
Vector Backbone Description: Backbone Size:2860; Vector Backbone:pRSETb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101193 Copy
Species: Other
Genetic Insert: SpCas9 variant G582A/V583A/E584A/D585A/N588A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101233 Copy
Species: Other
Genetic Insert: SpCas9 variant F491A/M495A/T496A/N497A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101235 Copy
Species: Synthetic
Genetic Insert: LOV2 and STIM1 cytosolic fragment
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101245 Copy
Species: Homo sapiens
Genetic Insert: METTL13
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL13:PBC007-B09:C235684. SGC or PDB link: http://www.thesgc.org/structures/5WCJ/
Proper citation: RRID:Addgene_101270 Copy
Species: Other
Genetic Insert: 4PY6
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6
Proper citation: RRID:Addgene_101272 Copy
Species: Homo sapiens
Genetic Insert: Utrophin
Vector Backbone Description: Vector Backbone:pCS2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101279 Copy
Species: Other
Genetic Insert: LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101372 Copy
Species: Other
Genetic Insert: RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101373 Copy
Species: Other
Genetic Insert: Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101377 Copy
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