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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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MG1655 ΔendA ΔrecA Resource Report Resource Website |
RRID:Addgene_37853 | None | PMID:20643967 | Vector Backbone:N/A; Vector Types:Other, E. coli bacterial strain; Bacterial Resistance:None | 2023-04-05 07:51:53 | 0 | ||||
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MG1655 ΔendA ΔrecA (DE3) Resource Report Resource Website |
RRID:Addgene_37854 | None | PMID:21110891 | Vector Backbone:N/A; Vector Types:Other, E. coli bacterial strain; Bacterial Resistance:None | 2023-04-05 07:51:53 | 0 | ||||
|
KI (37)-Oplac2 Resource Report Resource Website |
RRID:Addgene_52704 | KI (37)-Oplac2 strain | None | PMID:22605776 | To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of Oplac2 with those of KIlac, producing and KI(37)-Oplac2. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
|
degtag Resource Report Resource Website |
RRID:Addgene_52705 | degtag strain | None | PMID:22605776 | To generate the degtag strain, we introduced the ssrA tag to the C-terminus of LacZ, thus targeting the protein for degradation by the ClpXP and ClpAP proteases. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
|
KI (37)-Oplac1 Resource Report Resource Website |
RRID:Addgene_52703 | KI (37)-Oplac1 strain | None | PMID:22605776 | To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of OpLac1 with those of KIlac. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
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OpLac2-delta-6 Resource Report Resource Website |
RRID:Addgene_52700 | OpLac2-Δ6 strain | None | PMID:22605776 | Oplac2Δ6 was erroneously synthesized missing the first 6 nucleotides of Oplac2. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3. | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
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Oplac1 (37)-KI Resource Report Resource Website |
RRID:Addgene_52701 | Oplac1 (37)-KI strain | None | PMID:22605776 | To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of KILac with those of OpLac1. | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
|
S56L Resource Report Resource Website |
RRID:Addgene_52709 | S56L strain | None | PMID:22605776 | Impaired LacY function | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:44 | 0 | ||
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delta-Z Resource Report Resource Website |
RRID:Addgene_52706 | ΔZ strain | None | PMID:22605776 | lacZ deletion | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
|
G794D Resource Report Resource Website |
RRID:Addgene_52710 | G794D strain | None | PMID:22605776 | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:44 | 0 | |||
|
KIlac Resource Report Resource Website |
RRID:Addgene_52696 | KIlac strain | None | PMID:22605776 | KIlac refers to the knock-in of the wild-type operon into the attTn7 locus; this strain was used as the reference strain for all analyses. We constructed all knock-in strains using the approach of McKenzie and Craig. The knock-in contained the entire lac operon starting 75bp upstream of lacI and ending 100bp downstream of lacA. The ΔZYA strain was transformed with pGRG37. The plasmid carried both the lac operon and the transposon genes tnsABCD, which allow for a site-specific insertion at attTn7. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
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OpLac1 Resource Report Resource Website |
RRID:Addgene_52697 | OpLac1 strain | None | PMID:22605776 | The sequence Oplac1 was synthesized using preferred codons at every position (GENEART), thus optimizing the codon adaptation index. | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
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OpLac2 Resource Report Resource Website |
RRID:Addgene_52699 | OpLac2 strain | None | PMID:22605776 | Oplac2 was designed by a proprietary optimization strategy (developed by DNA 2.0) that incorporated codons for a subset of amino acids read by tRNAs that are the most highly charged during amino acid starvation. | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:43 | 0 | ||
|
HL 4233 Resource Report Resource Website |
RRID:Addgene_52944 | none | Other | None | PMID:25087841 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:56:46 | 0 | ||
|
HL 932 Resource Report Resource Website |
RRID:Addgene_52940 | none | Other | None | PMID:25087841 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:56:45 | 0 | ||
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HL3796 Resource Report Resource Website |
RRID:Addgene_52951 | none | Other | None | PMID: | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:56:46 | 0 | ||
|
HL4018 Resource Report Resource Website |
RRID:Addgene_52952 | none | Other | None | PMID: | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2023-04-05 07:56:46 | 0 | ||
|
JM109 Resource Report Resource Website 1+ mentions |
RRID:Addgene_49761 | Relevant genotype: recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15] | None | PMID:2985470 | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:17 | 1 | |||
|
JM107 Resource Report Resource Website |
RRID:Addgene_49759 | Relevant genotype: endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15] | Other | None | PMID:2985470 | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:17 | 0 | ||
|
JM106 Resource Report Resource Website |
RRID:Addgene_49757 | Relevant genotype: endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB) | Other | None | PMID:2985470 | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2023-04-05 07:56:17 | 0 |
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