Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Xenopus laevis
Genetic Insert: beta-catenin Delta N - 9 GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Beta-catenin repeats 10 - COOH fused to GFP.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16831 Copy
Species: Xenopus laevis
Genetic Insert: NLS beta-catenin GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: NLS of SV40.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16838 Copy
Species: Xenopus laevis
Genetic Insert: Flg-XDpr
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: 5' Flag tag on full length Xenopus Dapper (clone XE151) in pCS2+. Xba1 site is DAM methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16769 Copy
Species: Xenopus laevis
Genetic Insert: XDpr-delta PDZB
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcs2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full length Xenopus Dapper (clone XE151), with last four amino acids DELETED. Xba1 site is NOT methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16767 Copy
Species: Xenopus laevis
Genetic Insert: delta LZ-XDpr
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full length Xenopus Dapper (clone XE151), with deletion of first 129 amino acids, including the leucine zipper in pCS2+. Xba1 site is DAM methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16766 Copy
Species: Xenopus laevis
Genetic Insert: XDpr-delta PDZB
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Clone XE155 with a 5' GFP tag. The XDpr is truncated to delete the last four amino acids. Xba1 site is not methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16765 Copy
Species: Xenopus laevis
Genetic Insert: delta LZ-XDpr
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcs2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Clone XE156 with a 5- GFP tag. The XDpr is truncated to delete the first 129 amino acids; in pCS2+. Xba1 site is DAM methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16764 Copy
Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcs2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full length Xenopus Dapper, an inhibitor of Dsh signaling, as a 2.4kb EcoR1/Xba1 fragment in pCS2+. Xba1 site is DAM methylated.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16771 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta DIX
Vector Backbone Description: Backbone Size:4095; Vector Backbone:PCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: XDsh-GFP is from J. Yang-Snyder et al. (Curr. Biol., 1996).
A deletion of amino acids 1-159 was conducted and a new start met was introduced.
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16787 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-DEP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-433 and 503-736 (deleting 434-502).
Linearize with Not1 and transcribe with SP6.
Notes for Use:
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Please note that this gene matches NM_001090627.1, NOT BC090218.1.
Proper citation: RRID:Addgene_16785 Copy
Species: Xenopus laevis
Genetic Insert: XFz1-CS2P+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: PCR amplified coding region of Xenopus Frizzled-1 in Eco-XbaI.
site of CS2+.
Linearize with Asp718 to make sense RNA.
Ref: J. Brown et al., Development, Growth and Differentiation (2000).
Proper citation: RRID:Addgene_16793 Copy
Species: Xenopus laevis
Genetic Insert: XFz7-CS2P+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: PCR amplified coding region (BamHI-XbaI) of Xenopus Frizzled-7.
Linearize with Asp718 to transcribe sense RNA.
Notes for Use:
Ref: J. Brown et al., Development, Growth and Differentiation (2000).
Proper citation: RRID:Addgene_16792 Copy
Species: Xenopus laevis
Genetic Insert: Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Other, Zebrafish transgenesis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_198240 Copy
Species: Xenopus laevis
Genetic Insert: Xl-H3[ChEx]
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4640; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_221200 Copy
Species: Xenopus laevis
Genetic Insert: WBP2 N-terminal like
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_174791 Copy
Species: Xenopus laevis
Genetic Insert: caveolin1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONRp2R-p3; Vector Types:Other, Multisite gateway entry vector; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_194290 Copy
Species: Xenopus laevis
Genetic Insert: caveolin
Vector Backbone Description: Vector Backbone:pT3TS-Dest_R1-R3 (Addgene 140878); Vector Types:Other, In vitro transcription of mRNA; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_194293 Copy
Species: Xenopus laevis
Genetic Insert: Gene of metallothionein with Chiting Binding Domanin-Intein
Vector Backbone Description: Vector Backbone:pTYB21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_200293 Copy
Species: Xenopus laevis
Genetic Insert: BMP7
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Other, Xenopus expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_117256 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
