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Species: Other
Genetic Insert: RBD
Vector Backbone Description: Vector Backbone:pOP2A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site XhoI (not destroyed). Please visit https://doi.org/10.1101/2023.01.10.523494 for bioRxiv preprint.
Proper citation: RRID:Addgene_195000 Copy
Species: Other
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191284 Copy
Species: Other
Genetic Insert: SARS-CoV-2 BQ.1.1 Spike
Vector Backbone Description: Backbone Size:4716; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193710 Copy
Species: Other
Genetic Insert: Gene of metallothionein with Chiting Binding Domanin-Intein
Vector Backbone Description: Vector Backbone:pTYB21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_200292 Copy
Species: Other
Genetic Insert: Gene of metallothionein with Chiting Binding Domanin-Intein
Vector Backbone Description: Vector Backbone:pTYB21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_200295 Copy
Species: Other
Genetic Insert: Gene of metallothionein with Chiting Binding Domanin-Intein
Vector Backbone Description: Vector Backbone:pTYB21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_200298 Copy
Species: Homo sapiens
Genetic Insert: sgSMARCA4 guide 1
Vector Backbone Description: Vector Backbone:pXPR_003; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193604 Copy
Species: Mus musculus
Genetic Insert: ROSA-TRE-C16V5-G-pA
Vector Backbone Description: Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2022.11.21.517425 for bioRxiv preprint.
Proper citation: RRID:Addgene_194012 Copy
Species: Mus musculus
Genetic Insert: C16 Ex3 XTR f1
Vector Backbone Description: Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2022.11.21.517425 for bioRxiv preprint.
Proper citation: RRID:Addgene_194013 Copy
Species: Homo sapiens
Genetic Insert: ZNF7
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: XM_017013817,ENST00000325217 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_141601 Copy
Species: Homo sapiens
Genetic Insert: PURA
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: NM_005859,ENST00000331327 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_143866 Copy
Species: Homo sapiens
Genetic Insert: SMARCA4
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: XM_017027167,NM_001128847,ENST00000590574 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_143874 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pExp-CHis; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site HindIII (not destroyed).
Proper citation: RRID:Addgene_194998 Copy
Species: Homo sapiens
Genetic Insert: SPOP R221C
Vector Backbone Description: Vector Backbone:pEXP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.08.511432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_194116 Copy
Species: Homo sapiens
Genetic Insert: GLMP
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: NM_001256609,ENST00000614643 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_141746 Copy
Species: Homo sapiens
Genetic Insert: Telomere Maintenance 2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC-N MYC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22802 Copy
Species: Mus musculus
Genetic Insert: mmu-mir-137
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26327 Copy
Species: Homo sapiens
Genetic Insert: HK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23730 Copy
Species: Homo sapiens
Genetic Insert: TRPML1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_18824 Copy
Species: Homo sapiens
Genetic Insert: TRPML1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_18825 Copy
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