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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1 Resource Report Resource Website |
RRID:Addgene_187925 | hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1 | Synthetic | Ampicillin | PMID:35451955 | IS1 transposable element is between the two Bbs1 RE sites and will be removed when cloning in a gRNA sequence, and the ccdB selection still works. Please visit https://doi.org/10.1101/2021.07.21.453193 for bioRxiv preprint. | Vector Backbone:MIGRI; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:09 | 0 | |
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pTRIP-SFFV-GFP-RELA K5Q Resource Report Resource Website |
RRID:Addgene_196632 | GFP-RELA K5Q | Homo sapiens | Ampicillin | PMID:36820829 | Vector Backbone:pTRIP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | K122Q, K123Q, K310Q, K314Q, K315Q | 2024-08-01 01:05:37 | 0 | |
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pD649-HAsp-CD33-Fc(DAPA)-AviTag-6xHis Resource Report Resource Website |
RRID:Addgene_156694 | CD33 | Homo sapiens | Ampicillin | PMID:32822567 | Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter. | Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Fc region of human IgG contains D265A and P329A mutations (DAPA) | 2024-08-01 01:03:24 | 0 |
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pTRIP-SFFV-GFP-RELA K5R Resource Report Resource Website |
RRID:Addgene_196631 | GFP-RELA K5R | Homo sapiens | Ampicillin | PMID:36820829 | Vector Backbone:pTRIP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | K122R, K123R, K310R, K314R, K315R | 2024-08-01 01:05:37 | 0 | |
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pBS-TRE-tdKatushka2-WPRE Resource Report Resource Website |
RRID:Addgene_193337 | tdKatushka2 | Other | Ampicillin | PMID:38918382 | Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint. | Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:26 | 0 | |
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p663-UBC-miniTurbo-V5-NEK10_IDG-K Resource Report Resource Website |
RRID:Addgene_189874 | NEK10 | Homo sapiens | Ampicillin | PMID: | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:14 | 0 | |
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pBS-TRE-mNeonGreen-WPRE Resource Report Resource Website |
RRID:Addgene_193334 | mNeonGreen | Other | Ampicillin | PMID:38918382 | Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint. | Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:26 | 0 | |
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pBS-TRE-YPet-WPRE Resource Report Resource Website |
RRID:Addgene_193335 | YPet | Other | Ampicillin | PMID:38918382 | Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint. | Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:26 | 0 | |
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pBS-TRE-mRuby3-WPRE Resource Report Resource Website |
RRID:Addgene_193336 | mRuby3 | Other | Ampicillin | PMID:38918382 | Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint. | Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:26 | 0 | |
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pAGH10 - RlucFluc minus 1 HIV-1 Resource Report Resource Website |
RRID:Addgene_198224 | Renilla luciferase | Other | Ampicillin | PMID:36893757 | Backbone Marker:Addgene 12177; Backbone Size:3746; Vector Backbone:pIS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:42 | 0 | ||
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VR122 pETv2 10His-pG-Tn5 (E54K, L372P) Resource Report Resource Website |
RRID:Addgene_198467 | 10His-pG-Tn5 (E54K, L372P) | Synthetic | Kanamycin | PMID: | Please visit https://www.biorxiv.org/content/10.1101/2024.07.11.602973v1 for bioRxiv preprint. | Backbone Size:5234; Vector Backbone:pETv2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | E54K, L372P | 2024-08-01 01:05:43 | 0 |
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pBS-TRE-mTagBFP2-WPRE Resource Report Resource Website |
RRID:Addgene_193332 | mTagBFP2 | Other | Ampicillin | PMID:38918382 | Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint. | Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | A XhoI site in the coding sequence is silenced | 2024-08-01 01:05:26 | 0 |
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pAAV-CAG-hfYFP Resource Report Resource Website |
RRID:Addgene_191201 | Hyperfolder YFP | Synthetic | Ampicillin | PMID:36344833 | Constitutive expression | Backbone Marker:Scott Sternson; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105Y/K149N/T167I/H203Y/K206V/D234N | 2024-08-01 01:05:18 | 0 |
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TFORF0777 Resource Report Resource Website |
RRID:Addgene_142234 | SHOX | Homo sapiens | Ampicillin | PMID:36608654 | NM_000451,NM_000451,ENST00000381578,ENST00000554971,ENST00000381578,ENST00000554971 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence. | Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2024-08-01 01:02:39 | 0 | |
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pTETDuet_WTDHFR_Q33STS Resource Report Resource Website |
RRID:Addgene_191288 | folA, thyA | Other | Chloramphenicol | PMID:38637543 | Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint. | Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | WT DHFR, Q33S TYMS | 2024-08-01 01:05:19 | 0 |
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pTETDuet_F31YG121VDHFR_WTTS Resource Report Resource Website |
RRID:Addgene_191287 | folA, thyA | Other | Chloramphenicol | PMID:38637543 | Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint. | Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | F31Y/G121V DHFR, WT TYMS | 2024-08-01 01:05:18 | 0 |
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pFA6a-FKBP-GFP-HIS3MX6 Resource Report Resource Website |
RRID:Addgene_183023 | FKBP | Synthetic | Ampicillin | PMID:35496798 | Backbone Size:4698; Vector Backbone:pFA6a-GFP(S65T)-HIS3MX6; Vector Types:Other, Yeast Genomic Targeting; Bacterial Resistance:Ampicillin | 2024-08-01 01:04:52 | 0 | ||
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BcrMT1B Blue Crab Metallothionein 1B Resource Report Resource Website |
RRID:Addgene_200290 | Gene of metallothionein with Chiting Binding Domanin-Intein | Other | Ampicillin | PMID:37804185 | Vector Backbone:pTYB21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:49 | 0 | ||
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pTETDuet_WTDHFR_WTTS Resource Report Resource Website |
RRID:Addgene_191282 | folA, thyA | Other | Chloramphenicol | PMID:38637543 | Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint. | Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | WT DHFR, WT TYMS | 2024-08-01 01:05:18 | 0 |
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pPEPT1 Resource Report Resource Website |
RRID:Addgene_195001 | Ampicillin | PMID:38075664 | Plasmid was generated using LIC cloning with 5' site BsaI (destroyed in cloning) and 3' site BsaI (destroyed in cloning). | Vector Backbone:pExp-Chis; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:32 | 0 |
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