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Species: Synthetic
Genetic Insert: hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
Vector Backbone Description: Vector Backbone:MIGRI; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: IS1 transposable element is between the two Bbs1 RE sites and will be removed when cloning in a gRNA sequence, and the ccdB selection still works.
Please visit https://doi.org/10.1101/2021.07.21.453193 for bioRxiv preprint.
Proper citation: RRID:Addgene_187925 Copy
Species: Homo sapiens
Genetic Insert: GFP-RELA K5Q
Vector Backbone Description: Vector Backbone:pTRIP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_196632 Copy
Species: Homo sapiens
Genetic Insert: CD33
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_156694 Copy
Species: Homo sapiens
Genetic Insert: GFP-RELA K5R
Vector Backbone Description: Vector Backbone:pTRIP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_196631 Copy
Species: Other
Genetic Insert: tdKatushka2
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_193337 Copy
Species: Homo sapiens
Genetic Insert: NEK10
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189874 Copy
Species: Other
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_193334 Copy
Species: Other
Genetic Insert: YPet
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_193335 Copy
Species: Other
Genetic Insert: mRuby3
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_193336 Copy
Species: Other
Genetic Insert: Renilla luciferase
Vector Backbone Description: Backbone Marker:Addgene 12177; Backbone Size:3746; Vector Backbone:pIS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_198224 Copy
Species: Synthetic
Genetic Insert: 10His-pG-Tn5 (E54K, L372P)
Vector Backbone Description: Backbone Size:5234; Vector Backbone:pETv2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.07.11.602973v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_198467 Copy
Species: Other
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_193332 Copy
Species: Synthetic
Genetic Insert: Hyperfolder YFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Constitutive expression
Proper citation: RRID:Addgene_191201 Copy
Species: Homo sapiens
Genetic Insert: SHOX
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: NM_000451,NM_000451,ENST00000381578,ENST00000554971,ENST00000381578,ENST00000554971 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_142234 Copy
Species: Other
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191288 Copy
Species: Other
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191287 Copy
Species: Synthetic
Genetic Insert: FKBP
Vector Backbone Description: Backbone Size:4698; Vector Backbone:pFA6a-GFP(S65T)-HIS3MX6; Vector Types:Other, Yeast Genomic Targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183023 Copy
Species: Other
Genetic Insert: Gene of metallothionein with Chiting Binding Domanin-Intein
Vector Backbone Description: Vector Backbone:pTYB21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_200290 Copy
Species: Other
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191282 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pExp-Chis; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid was generated using LIC cloning with 5' site BsaI (destroyed in cloning) and 3' site BsaI (destroyed in cloning).
Proper citation: RRID:Addgene_195001 Copy
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